BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0133.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 924 0.0 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 139 5e-34 gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 124 2e-29 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 117 2e-27 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 103 3e-23 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 924 bits (2387), Expect = 0.0, Method: Compositional matrix adjust. Identities = 453/453 (100%), Positives = 453/453 (100%) Query: 1 MKVRTQSGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 60 MKVRTQSGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT Sbjct: 3 MKVRTQSGWFSNGLFWRTFFLLTFLITASMVAWVASFRMVERGPRAEQLAAQIVSVVTIT 62 Query: 61 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT 120 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT Sbjct: 63 RAALTHSAPEMRRELLFDLASNEGIRIYPLEKSDRIVPPEDSPIVPELEYYVRQSLDENT 122 Query: 121 LFASSVNDVEGFWISFNIDDDKYWLMLDRGRLDRTSSLQWLGWGSIALFLSLIGAVFIST 180 LFASSVNDVEGFWISFNIDDDKYWLMLDRGRLDRTSSLQWLGWGSIALFLSLIGAVFIST Sbjct: 123 LFASSVNDVEGFWISFNIDDDKYWLMLDRGRLDRTSSLQWLGWGSIALFLSLIGAVFIST 182 Query: 181 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG 240 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG Sbjct: 183 LINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAG 242 Query: 241 ISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDL 300 ISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDL Sbjct: 243 ISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDL 302 Query: 301 ADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVENARRYGKTEGTDCAE 360 ADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVENARRYGKTEGTDCAE Sbjct: 303 ADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGTELARVFSNLVENARRYGKTEGTDCAE 362 Query: 361 IDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL 420 IDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL Sbjct: 363 IDIRSRIEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTARGQANGSGLGLAIVNRIVL 422 Query: 421 KHNGKLLLKGHEETRSGLLVQITLPLQGHRRHA 453 KHNGKLLLKGHEETRSGLLVQITLPLQGHRRHA Sbjct: 423 KHNGKLLLKGHEETRSGLLVQITLPLQGHRRHA 455 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 139 bits (350), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 138/472 (29%), Positives = 213/472 (45%), Gaps = 54/472 (11%) Query: 10 FSNGLFWRTFFLLTFLITASMVA--WVAS--FRMVERGPRAEQLAAQIVSVVTITRAALT 65 F + R FFLL I ++ A W+A R R R + LA ++ +V Sbjct: 4 FLGSIGNRIFFLLLGGIVVAVFATNWLAGNERRNTLRELRFQHLADRVEQIVQAV----- 58 Query: 66 HSAPEMRRELLFDLASNEGIRIYPLEK-SDRIVP-----PEDSPIVPELEYYV------- 112 + P +R ++ ASN G +E +D I P P SP+ L+ + Sbjct: 59 DNIPPAQRPIVLQAASNFGFEARMVEDMNDAIGPDASPSPSTSPLTEMLQARLGEDRKIA 118 Query: 113 --RQS----LDENTLFASSVNDVEGFWISFNIDDDKYWLMLD--------RGRLDRTSSL 158 R+S L + A + +IS + D L L R R Sbjct: 119 VQRESECPPLRTDDARAPRRDTCRVIYISLH-DQALMRLRLHQPGEPPALRARAGPGGPA 177 Query: 159 QWLGWGSIALFLSLIG--AVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEA 216 G I LFL LI A ++ + +P+ +L AA + PL E+GPTEI +A Sbjct: 178 SLFGLQYILLFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQA 237 Query: 217 NRSFNQMVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEA-RDGMHSDLAQ 275 +FN M A + R R +LA I+HDL+TPL RM+L +E +SD + + DL Sbjct: 238 AHAFNAMQARIRRQIQHRTHMLAAITHDLQTPLTRMRLRLE--KVSDPGLQQKLIDDLGV 295 Query: 276 MDSIIGQFLDYAKPFDTS-SLETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVAGNGT 334 M ++ + LD A+ D+S ++ +D+ +L V +A+ V Q + V Sbjct: 296 MHGMVREGLDLARSMDSSEKIQALDIDSLLDSVCADAADAGQ-DVALQGSTRAFVMAQPG 354 Query: 335 ELARVFSNLVENARRYGKTEGTDCAEIDIRSRIEGHQVVVEVADHGPGVPVNECERLLRP 394 L R +NL++NA +YGK+ A +E ++ + + D GPG+P +E E + P Sbjct: 355 ALRRCLTNLIDNACKYGKSARVSIA-------LEQQKIAIRIRDQGPGIPESELEAVFEP 407 Query: 395 FTRLDTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 445 F RL+ +R + G+GLGL I I H+G+L L+ E GL V + LP Sbjct: 408 FYRLEASRSRDTGGTGLGLTIALNIAENHHGQLRLRNLAE--GGLEVLLELP 457 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 124 bits (311), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 90/254 (35%), Positives = 120/254 (47%), Gaps = 14/254 (5%) Query: 196 IAKGRQPEPLPESGPTEIEEANRSFNQMVADLNRIESDRALILAGISHDLRTPLARMQLE 255 KG LPE+G E + R+F+ M + R DR + ISHDLRTP+ R++L Sbjct: 233 FGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLR 292 Query: 256 VEMANLSDEARDGMHSDLAQMDSIIGQFLDYAKPFDTSSLETIDLADVLQDVAGNASRLS 315 E+ + D R H DL ++D ++ L K D T D L +RL+ Sbjct: 293 TELMD-DDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLA 351 Query: 316 DIRVHTQITPD-TQVAGNGTELARVFSNLVENARRYGKTEGTDCAEIDIRSR--IEGHQV 372 + TP V L R NL +NA YG+ EI IR EG V Sbjct: 352 GHEI--AFTPSGINVIAKPLALKRAIGNLFDNALYYGQK-----VEISIRPSPGAEGDLV 404 Query: 373 VVEVADHGPGVPVNECERLLRPFTRLDTARGQ-ANGSGLGLAIVNRIVLKHNGKLLLKGH 431 +E+ DHGPGVP + L +P+ RL+ R Q + G GLGL I IV H G+LLL+ H Sbjct: 405 EIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNH 464 Query: 432 EETRSGLLVQITLP 445 E GL+ I LP Sbjct: 465 PE--GGLMATIILP 476 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 117 bits (294), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 92/291 (31%), Positives = 139/291 (47%), Gaps = 30/291 (10%) Query: 165 SIALFLSLIGAVFISTLINQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMV 224 ++ +F ++ A L +PL + AA + + PL GP E+ A ++FN M Sbjct: 208 ALRIFAVVLIAWMAVRLAMKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQ 267 Query: 225 ADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDE-ARDGMHSDLAQMDSIIGQF 283 + ++R LA +SHDLR+P+ RM+L EM L DE ++ DL +MD++I Sbjct: 268 RRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEM--LPDERGKEKFRKDLDEMDALISAT 325 Query: 284 LDYAKPFDTSSL-ETIDLADVLQDVAGNASRLSDIRVHTQITPDTQVA---GNGTELARV 339 L+ + D + IDL +L+ + R V ++T A G L R Sbjct: 326 LNVVQDIDVQERPQLIDLDSLLESL-----RTDQAEVGGEVTLSGHAAPLQGYPRSLRRC 380 Query: 340 FSNLVENARRYGKTEGTDCAEIDIRSRIE----GHQVVVEVADHGPGVPVNECERLLRPF 395 NLVENA RYG+ R+R++ QV + V D GPG+P ER+ PF Sbjct: 381 LQNLVENAVRYGQ-----------RARVQLQDDADQVCIRVDDDGPGIPEALLERVQEPF 429 Query: 396 TRLDTARGQANGS-GLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 445 RL+ +R G GLGL+I +V H G ++K GL V+I +P Sbjct: 430 YRLERSRNAGTGGFGLGLSIAATVVQAHRG--VMKLVNRPGGGLRVEILIP 478 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 103 bits (257), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 80/297 (26%), Positives = 143/297 (48%), Gaps = 22/297 (7%) Query: 166 IALFLSLIGAVFISTLINQPLARLTAAARAIAKGR---QPEPLPESGPTEIEEANRSFNQ 222 +AL SL+ A+ ++ + P+ L A A G + EP+ + E+ + R F+ Sbjct: 170 VALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLGRDFDV 229 Query: 223 MVADLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEAR-----DGMHSDLAQMD 277 M A L + + +L +SH+LR+P+AR+Q+ + +A + D+ + + + + +MD Sbjct: 230 MTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIERESVRMD 289 Query: 278 SIIGQFLDYAK---PFDTSSLETIDLADVLQDVAGNASRLSDI---RVHTQITPDTQVAG 331 ++G+ L ++ S E I + +L D+ +AS ++ V Q+ QV G Sbjct: 290 KLVGELLTLSRLEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCRVQVKG 349 Query: 332 NGTELARVFSNLVENARRYGKTEGTDCAEIDIRSRIE--GHQVVVEVADHGPGVPVNECE 389 N L R N++ NA ++ T + I ++++ + + + D GPGVP E Sbjct: 350 NPELLYRAIENVLRNAIKHTAPGTT----VQIHTQLDEINKLLHLSILDRGPGVPEEEMN 405 Query: 390 RLLRPFTRLDTARGQANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLPL 446 + F R + ++G GLGL+I RIV H G ++ + GL V ITLP Sbjct: 406 SIFELFFRSSRTKSNSSGHGLGLSIARRIVEAHQG--FIQAANRSNGGLRVDITLPF 460 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,299,108 Number of extensions: 50939 Number of successful extensions: 312 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 269 Number of HSP's successfully gapped: 31 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)