BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0130.p (219 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311471|ref|YP_003775563.1| amino acid ABC transporter perm... 435 e-123 gi|300313611|ref|YP_003777703.1| amino acid ABC transporter perm... 133 1e-32 gi|300309410|ref|YP_003773502.1| glutamine ABC transporter perme... 131 4e-32 gi|300310828|ref|YP_003774920.1| amino acid ABC transporter perm... 130 8e-32 gi|300313167|ref|YP_003777259.1| amino acid ABC transporter perm... 127 5e-31 >gi|300311471|ref|YP_003775563.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 221 Score = 435 bits (1118), Expect = e-123, Method: Compositional matrix adjust. Identities = 219/219 (100%), Positives = 219/219 (100%) Query: 1 MEILELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRPIHWPAAIYVSM 60 MEILELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRPIHWPAAIYVSM Sbjct: 3 MEILELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRPIHWPAAIYVSM 62 Query: 61 MRGTPLLVQIFVVYYGLPGMGIEFSPLTAGVLTLSLNASAYLSESLRGAILGVTRGQWNA 120 MRGTPLLVQIFVVYYGLPGMGIEFSPLTAGVLTLSLNASAYLSESLRGAILGVTRGQWNA Sbjct: 63 MRGTPLLVQIFVVYYGLPGMGIEFSPLTAGVLTLSLNASAYLSESLRGAILGVTRGQWNA 122 Query: 121 AFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLIKDTSLVSVITVTELMLATKEVIAV 180 AFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLIKDTSLVSVITVTELMLATKEVIAV Sbjct: 123 AFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLIKDTSLVSVITVTELMLATKEVIAV 182 Query: 181 TFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKAHQI 219 TFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKAHQI Sbjct: 183 TFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKAHQI 221 >gi|300313611|ref|YP_003777703.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 247 Score = 133 bits (334), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 129/241 (53%), Gaps = 27/241 (11%) Query: 5 ELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIV-----PWR----PIHWPAA 55 ++Q AP AKGV TL +L S+L G V+G ++ + R+ PW+ + W A Sbjct: 6 SIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKSMRWLAG 65 Query: 56 IYVSMMRGTPLLVQIFVVYYGL--------PGMGI----------EFSPLTAGVLTLSLN 97 YV+ RGTPL VQI ++++ + G+ I ++ +G + LSLN Sbjct: 66 FYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVALSLN 125 Query: 98 ASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLIK 157 A AY+SE R I + RGQ AA S+G+ Y T+RY+++PQA R +P + N I+L+K Sbjct: 126 AGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAITLLK 185 Query: 158 DTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKAH 217 D+SLVS I + EL A + V R Y+ + +Y+ +++C + RLE K H Sbjct: 186 DSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKYSAPH 245 Query: 218 Q 218 + Sbjct: 246 R 246 >gi|300309410|ref|YP_003773502.1| glutamine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 250 Score = 131 bits (330), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 121/241 (50%), Gaps = 28/241 (11%) Query: 5 ELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRP----------IHWPA 54 E++ + P +G TL A+ ++ G G VL V R+ R + WP Sbjct: 8 EIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLCYCVQWPI 67 Query: 55 AIYVSMMRGTPLLVQIFVVYYGL------PGMGI------------EFSPLTAGVLTLSL 96 YVS +RGTPL VQI ++++ L P G+ + + VL ++L Sbjct: 68 RFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLAITL 127 Query: 97 NASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLI 156 N+ AY+SE R I + RGQ A+ S+G++Y TLR +++PQA R +P + N I+++ Sbjct: 128 NSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAIAIV 187 Query: 157 KDTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKA 216 KD+SL S I + EL A + V R Y+ + IYW+++L AL LE + GK Sbjct: 188 KDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLEARYGKG 247 Query: 217 H 217 Sbjct: 248 D 248 >gi|300310828|ref|YP_003774920.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 216 Score = 130 bits (327), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 72/205 (35%), Positives = 122/205 (59%), Gaps = 1/205 (0%) Query: 14 LAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRPIHWPAAIYVSMMRGTPLLVQIFVV 73 L + +TLL + + LGG + GF++A+ R P+ +A+Y+ +++GTP+L+ +F+ Sbjct: 13 LLEAARWTLLLSALAFLGGGIAGFIVALMRTSHLAPLRLLSAVYIQIIQGTPVLILLFLS 72 Query: 74 YYGLPGMGIEFSPLTAGVLTLSLNASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLR 133 +YGL G + P+ A + +++ +SAYL E RG I V QW A+ ++ +T +Q LR Sbjct: 73 FYGLAIFGYKLPPMVAATIAMTIYSSAYLGEIWRGCIEAVPVPQWEASTALAMTRWQQLR 132 Query: 134 YIIVPQAVRIAVPSMSNTLISLIKDTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAA 193 Y+I+PQAVRI++P L+ ++K+TS+ S+I + EL A K V TF+P ++ A Sbjct: 133 YVILPQAVRISLPPTVGFLVQIVKNTSIASIIGLVELAQAGKLVSNATFQPFLVFPIVAG 192 Query: 194 IYWMLSLCFEALQHRLEKKLGKAHQ 218 IY++ LE+K AH+ Sbjct: 193 IYFVFCYPLSRFAFALERKY-HAHR 216 >gi|300313167|ref|YP_003777259.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 216 Score = 127 bits (320), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 69/199 (34%), Positives = 116/199 (58%) Query: 20 YTLLFALASMLGGLVLGFVLAVARIVPWRPIHWPAAIYVSMMRGTPLLVQIFVVYYGLPG 79 +T++ +L + + G V GFV+A+ R+ P + A+ Y+ +++G PLLV +FV Y+GL Sbjct: 18 WTVVLSLVAFVFGGVAGFVIALWRVSPNAVLRGIASGYIQVVQGIPLLVILFVAYFGLAI 77 Query: 80 MGIEFSPLTAGVLTLSLNASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLRYIIVPQ 139 G + +PL A ++ ++ +A+L E RG I V + QW A+ +G F+ L +I+PQ Sbjct: 78 AGFKLTPLVAAGISFAIYCAAFLGEIWRGCIQAVPKTQWEASECLGFNRFEQLTKVILPQ 137 Query: 140 AVRIAVPSMSNTLISLIKDTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAAIYWMLS 199 AV+IA P ++ ++K+TSL SVI +L A + + TF+P ++ A IY+ L Sbjct: 138 AVKIATPPTVGFMVQIVKNTSLASVIGFVDLSRAGQIINNSTFQPFTVFGCVALIYFCLC 197 Query: 200 LCFEALQHRLEKKLGKAHQ 218 AL E+KL +H+ Sbjct: 198 FPLSALSKHFERKLNVSHR 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 596,285 Number of extensions: 22002 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 19 Length of query: 219 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 137 Effective length of database: 1,231,600 Effective search space: 168729200 Effective search space used: 168729200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 128 (53.9 bits)