BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0123.p (432 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotr... 835 0.0 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 119 5e-28 gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent a... 88 1e-18 gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Her... 83 4e-17 gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic ami... 65 1e-11 >gi|300311478|ref|YP_003775570.1| succinyldiaminopimelate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 420 Score = 835 bits (2157), Expect = 0.0, Method: Compositional matrix adjust. Identities = 409/411 (99%), Positives = 409/411 (99%) Query: 2 LAREALLQSSAVNPLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQ 61 LAREALLQSSAVNPLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQ Sbjct: 7 LAREALLQSSAVNPLLDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTPAFIQQ 66 Query: 62 ALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQT 121 ALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQT Sbjct: 67 ALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQT 126 Query: 122 VVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQL 181 VVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQL Sbjct: 127 VVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQL 186 Query: 182 LFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYSEIYVNEDPPLGGMQAARLLG 241 LFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYSEIY NEDPPLGGMQAARLLG Sbjct: 187 LFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYSEIYFNEDPPLGGMQAARLLG 246 Query: 242 RDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ 301 RDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ Sbjct: 247 RDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQ 306 Query: 302 DETHVQDNIAKYVTKFSQVTPLLQEVLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAE 361 DETHVQ NIAKYVTKFSQVTPLLQEVLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAE Sbjct: 307 DETHVQGNIAKYVTKFSQVTPLLQEVLEVALPDAAFYLWAKVDKLVNISDTEFAQRLYAE 366 Query: 362 YNVTVLPGSYLAREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLA 412 YNVTVLPGSYLAREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLA Sbjct: 367 YNVTVLPGSYLAREAHGVNPGAGRIRMALVAEVEECLEAAQRIVAFTRKLA 417 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 119 bits (298), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 89/333 (26%), Positives = 160/333 (48%), Gaps = 17/333 (5%) Query: 44 ISLGIGEPKHPTPAFIQQALID--NLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDP 101 I + +G P TP I + L++ Y ++ G LR AIA W + RY++ DP Sbjct: 70 IDMSMGNPDGATPPHIVEKLVEVAQRPDTHGYSSSKGIPRLRRAIAHWYRSRYEVDF-DP 128 Query: 102 ATEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPK 161 +E + GS+E L L +D G V+ PNP Y I+ A +AGA V P Sbjct: 129 DSEAIVTIGSKEGLAHLMLATLDK---GDTVLVPNPSYPIHIYGAVIAGANIRSVRMSPG 185 Query: 162 RNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYS 221 +F + + ++ + + +++ + P NPT + LE ++ + L+ + ++ D Y+ Sbjct: 186 VDFFDELERAVRESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVHDLAYA 245 Query: 222 EIYVN--EDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLL 279 +I + + P + + AR + + F +LSK N+ G R GF+ G+A ++ Sbjct: 246 DITFDGWKAPSIMQVPGAREVA------VEFFTLSKSYNMAGWRIGFMVGNARLVAALAR 299 Query: 280 YRTYHGAAMSPVIQAASVLAWQ-DETHVQDNIAKYVTKFSQVTPLLQEV-LEVALPDAAF 337 ++YH +Q A++ A + D++ V++ A Y + + + L E V +P A+ Sbjct: 300 IKSYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLVKGLHEAGWMVDVPKASM 359 Query: 338 YLWAKV-DKLVNISDTEFAQRLYAEYNVTVLPG 369 Y+WA++ + EFA+ L + V V PG Sbjct: 360 YIWARIPEPYRQFGSLEFARILLEQAKVCVSPG 392 >gi|300312316|ref|YP_003776408.1| pyridoxal phosphate dependent aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 85/330 (25%), Positives = 139/330 (42%), Gaps = 11/330 (3%) Query: 44 ISLGIGEPK-HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRYDIPRPDPA 102 ++LG G P H PA + GL YP G LR AIA+ ++K Y R DP Sbjct: 31 VNLGQGFPDFHCDPALVAAVTQAMQEGLNQYPPMAGVPVLREAIAEKVEKLYG-HRYDPV 89 Query: 103 TEILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSD-PK 161 +EI G+ + + + +V+ PG V+ P Y Y + LAG P FV + Sbjct: 90 SEITVTAGATQGILT---SVLCAVHPGDEVIVIEPVYDCYVPAIELAGGVPVFVQMEVGA 146 Query: 162 RNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGFVIASDECYS 221 + ++ + +V V + +++ V +P NPTG+V+ D L + +I SDE Y Sbjct: 147 QGYSIPWDKVKAAVTPKTRMIMVNTPHNPTGSVMRAADVAALADIVRGTDILILSDEVYE 206 Query: 222 EIYVNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYR 281 + + P + L R + SS K +V G + G+VA AA+ +F Sbjct: 207 HMVYDGQPHESLARHPELAERSFIN----SSFGKTYHVTGWKVGYVAAPAALTAEFRKVH 262 Query: 282 TYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQV-TPLLQEVLEVALPDAAFYLW 340 ++ ++ +Q +D D A Y K T L E+ ++ Sbjct: 263 QFNVFTVNTPVQYGLAAYMKDPAPYLDLPAFYQHKRDLFRTGLANTRFELLPSQGTYFQC 322 Query: 341 AKVDKLVNISDTEFAQRLYAEYNVTVLPGS 370 K + + + EF + L +E V +P S Sbjct: 323 VKYGAISALPEAEFCKWLTSEIGVAAIPVS 352 >gi|300311409|ref|YP_003775501.1| aspartate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 409 Score = 83.2 bits (204), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 89/389 (22%), Positives = 168/389 (43%), Gaps = 39/389 (10%) Query: 38 DPAYRPISLGIGEPK---HPTPAFIQQALIDNLAGLASYPATLGSDALRGAIADWLQKRY 94 + ++ I L IG P I Q +I N+ + Y + G A R ++ + Q++ Sbjct: 30 EEGHKIIKLNIGNLAAFGFDAPDEIVQDMIRNMGNASGYTDSKGLFAPRKSVMHYTQQK- 88 Query: 95 DIPRPDPAT--EILPVNGSREALFSLTQTVVDPTRPGALVMCPNPFYQIYEGSAYLAGAQ 152 + + T +I NG+ E + +++ G V+ P+P Y ++ + L+G Sbjct: 89 ---KIEGVTIDDIYLGNGASELIVMSVNALLNT---GDEVLVPSPDYPLWTAAVSLSGGT 142 Query: 153 PYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVCSPGNPTGAVLTLEDWKELFALSDRYGF 212 P D ++ + PD + + + + + + V +P NPTGA+ ++E KE+ L+ ++ Sbjct: 143 PVHYVCDEQQGWQPDIADIKKKITPNTKAIVVINPNNPTGALYSVEVLKEIIELARQHQL 202 Query: 213 VIASDECYSEIYVNEDPPLGGMQAARLLGRDYRRLISFSSLSKRSNVPGMRSGF--VAGD 270 +I +DE Y ++ + G + D I+F+ LSK G R+G+ V+G+ Sbjct: 203 IILADEIYDKVLYD-----GNTHTSLASLADDVLFITFNGLSKNYRSCGYRAGWMVVSGE 257 Query: 271 AAILKKFLLYRTYHGAAMSPVIQAASVLAWQDETHVQDNIAKYVTKFSQVTP-------L 323 + ++ A+M A A Q +I V ++T L Sbjct: 258 KKHARDYIEGLNML-ASMRLCANAPGQFAIQTALGGYQSINDLVGPQGRLTKQRDLAHQL 316 Query: 324 LQEVLEVAL--PDAAFYLWAKVDKLVN--ISDTEFAQRLYAEYNVTVLPGSYLAREAHGV 379 L ++ V+ P +A Y++ ++D + D EFA L AE V ++ G+ G Sbjct: 317 LTDIPGVSCVKPKSALYMFPRLDPEIYPIKDDQEFAYELLAEEKVLIVQGT-------GF 369 Query: 380 N-PGAGRIRMALVAEVEECLEAAQRIVAF 407 N P R+ + ++ E+ RI F Sbjct: 370 NCPTPDHFRVVFLPNTDDLTESMGRIAHF 398 >gi|300312878|ref|YP_003776970.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 393 Score = 64.7 bits (156), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 97/412 (23%), Positives = 162/412 (39%), Gaps = 51/412 (12%) Query: 17 LDQLQPYPFEKLKKLFAGITPDPAYRPISLGIGEPKHPTP-AFIQQALIDNLAGLASYPA 75 L + P+ +L K A + I +GIGEP P A ++ A G Y + Sbjct: 10 LQHIAPFHVMELSKK-AAVLEQQGRHLIHMGIGEPDFTAPPAVVEAATRAMTEGKMQYTS 68 Query: 76 TLGSDALRGAIADWLQKRYDIPRPDPATEILPVNGSREALFSLTQTVV----------DP 125 G LR AI+D + Y + + A E + V A L + DP Sbjct: 69 ATGLPQLRAAISDHYRSVYGL---EIAPERIVVTAGASAALLLACAALVERDSEVLMPDP 125 Query: 126 TRPGALVMCPNPFYQIYEGSAYLAGAQPYFVNSDPKRNFAPDFSQVPQDVWERVQLLFVC 185 + P C F +EG A L + S P+ F V + + + + Sbjct: 126 SYP-----CNRHFVAAFEGRAKL-------IASGPEHRFQLSAQMVREHWGSATRGVLLA 173 Query: 186 SPGNPTGAVLTLEDWKELFA-LSDRYGFVIASDECYSEIYVNEDPPLGGMQAARLLGRDY 244 SP NPTG + ++ + + + R GF I DE Y + E P + LG D Sbjct: 174 SPSNPTGTSILPDELRAIVGEVRQRGGFTIV-DEIYQGLSY-EGAPFSALS----LGDD- 226 Query: 245 RRLISFSSLSKRSNVPGMRSGFVAGDAAILKKFLLYRTYHGAAMSPVIQAASVLAWQDET 304 ++ +S SK N+ G R G++ A++ + S + Q A+V + +T Sbjct: 227 --VVVINSFSKYFNMTGWRLGWLVLPPALVPQVEKLAQNLLICASSIAQHAAVACFTPQT 284 Query: 305 HV--QDNIAKYVTKFSQVTPLLQEVLEV--ALPDAAFYLWAKVDKLVNISDTEFAQRLYA 360 ++ A++ + + P L+ + +PD AFY++A L + +D + + + Sbjct: 285 LALYEERKAEFKRRRDYIVPALESLGFTVPVMPDGAFYVYADCSALADDAD-QLSLDMLN 343 Query: 361 EYNVTVLPGSYLAREAHGVNPGAGR--IRMALVAEVEECLEAAQRIVAFTRK 410 E V ++PG P R IR++ +E EA R+ F K Sbjct: 344 EAGVVLVPGL-------DFGPFTARRYIRLSYATSMENLQEAVARLRVFFEK 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,442,771 Number of extensions: 62151 Number of successful extensions: 168 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 5 Length of query: 432 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 344 Effective length of database: 1,203,190 Effective search space: 413897360 Effective search space used: 413897360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)