BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0090.p (784 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/py... 1261 0.0 gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein pho... 84 4e-17 >gi|300311510|ref|YP_003775602.1| phosphoenolpyruvate synthase/pyruvate phosphate dikinase [Herbaspirillum seropedicae SmR1] Length = 791 Score = 1261 bits (3264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 618/620 (99%), Positives = 619/620 (99%) Query: 1 MTDVESVGGKNASLGEMISQLAGAGARVPGGFATTAQAFRDFLEHSADGGPTLAARIAKR 60 MTDVESVGGKNASLGEMISQLAGAG RVPGGFATTAQAFRDFLEHSADGGPTLAARIAKR Sbjct: 21 MTDVESVGGKNASLGEMISQLAGAGVRVPGGFATTAQAFRDFLEHSADGGPTLAARIAKR 80 Query: 61 LEGLDIDDVRSLAQAGAEIRQWIIDTPFQPRLDQEIRAFYDKLVADSTAEMSFAVRSSAT 120 LEGLDIDDVRSLAQAGAEIRQWIIDTPFQPRLDQEIRAFYDKLVADSTAEMSFAVRSSAT Sbjct: 81 LEGLDIDDVRSLAQAGAEIRQWIIDTPFQPRLDQEIRAFYDKLVADSTAEMSFAVRSSAT 140 Query: 121 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG 180 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG Sbjct: 141 AEDLPDASFAGQQETFLNVVGIDNVLEAMKHVFASLYNDRAISYRVHKGFTHAEVALSAG 200 Query: 181 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDEYYVHKPMLELG 240 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDEYYVHKPMLELG Sbjct: 201 VQRMVRSDVGAAGVMFTIDTESGFKDVVFITSSYGLGETVVQGAVNPDEYYVHKPMLELG 260 Query: 241 KQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNRYSLTDAEVVELAKYATIIEK 300 KQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNRYSLTDAEVVELAKYATIIEK Sbjct: 261 KQPVIRRNLGSKLIKMEFTGEAKAGRSVKTVDVPVELRNRYSLTDAEVVELAKYATIIEK 320 Query: 301 HYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLKSSGTVLATGRAIGQK 360 HYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLKSSGTVLATGRAIGQK Sbjct: 321 HYQRPMDIEWGKDGRDGKLYILQARPETVKSQQKATDAQQRFKLKSSGTVLATGRAIGQK 380 Query: 361 IGAGPVRVITDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARE 420 IGAGPVRVITDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARE Sbjct: 381 IGAGPVRVITDPAEMERVQPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARE 440 Query: 421 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETEVTEVARGELPPIPLKVMM 480 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETEVTEVARGELPPIPLKVMM Sbjct: 441 LGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIYDGLLETEVTEVARGELPPIPLKVMM 500 Query: 481 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGH 540 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGH Sbjct: 501 NVGNPQLAFDFQSIPNGGVGLARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGH 560 Query: 541 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR 600 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR Sbjct: 561 ASPKAFYVDKLAEGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFR 620 Query: 601 GAARYLAEDFAEAFQMECQA 620 GAARYLA+DFAEAFQMECQA Sbjct: 621 GAARYLADDFAEAFQMECQA 640 >gi|300309436|ref|YP_003773528.1| phosphoenolpyruvate-protein phosphotransferase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 595 Score = 84.0 bits (206), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 95/410 (23%), Positives = 176/410 (42%), Gaps = 81/410 (19%) Query: 404 VTNRGGRTCHAAIIARELGVPAVVGCGDATDVLKDGTLVTVSCAEGDEGKIY-------- 455 VT+ GG H AI+AR L +PA VG +A+ ++K ++ + +GD G + Sbjct: 186 VTDLGGPNSHTAIVARSLAIPAAVGMHNASVLVKHDDVLII---DGDAGVVIVDPSPLVL 242 Query: 456 ------DGLLETEVTEVARGELPPI------PLKVMMNV---GNPQLAFDFQSIPNGGVG 500 L E +++R + P P+ ++ N+ G+ AF+ ++ G+G Sbjct: 243 AQYRARQERLLRERKKLSRLKKTPAITQDGEPITLLANIELPGDAVTAFEAGAM---GIG 299 Query: 501 LARLEFIINNNIGVHPKAILEYPNIDADLKKAVESVARGHASPKAFYVDKLAEGIATIAA 560 L R EF+ G + ++P+ D + ++V Sbjct: 300 LFRSEFLFMGRAG----HLEKFPSEDEQFEHYKQAVI----------------------- 332 Query: 561 AFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEE---NPMLGFRGAARYLAEDFAEAFQME 617 A + V +R D +++ + DEE NP LG R L+E QM Sbjct: 333 AMKGRSVTIRTLDVGADK--------PLDEDEETALNPALGLRAIRYCLSEP-----QMF 379 Query: 618 CQAMKRVREDMGLTNVEVMVPFVRTLGQAEKVIGLL--GKYGLK----RGENGLRVIMMC 671 ++ + ++++VP + + ++ + ++ K L+ + + + V M Sbjct: 380 LTQLRAILRASAFGPIKLLVPMMAHAFEIDQTLAMIDQAKQQLRERKQKFDEEIPVGAMI 439 Query: 672 EVPSNAILAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGMELLAADFDERDPALQALLSKV 731 E+P+ A+ F++ D SIG+NDL Q TL +DR +A +D PA+ LLS V Sbjct: 440 EIPAAALALPLFVKRLDFLSIGTNDLIQYTLAIDRVD--HEVAHLYDPLHPAVLFLLSTV 497 Query: 732 IATCREKGKYVGICGQGPSDHPDFAEWLMEQGIESISLNPDTVIDTWQQL 781 I+ R+ G V +CG+ D + L+ G+ S++P ++ ++ Sbjct: 498 ISQGRKAGIPVSVCGEMAGDL-KWTRLLLGMGLLEFSMHPSQILSVKNEI 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,485,798 Number of extensions: 109538 Number of successful extensions: 336 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 2 Length of query: 784 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 691 Effective length of database: 1,179,515 Effective search space: 815044865 Effective search space used: 815044865 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)