BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0070.p (524 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbasp... 1085 0.0 gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbasp... 301 1e-82 gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbasp... 239 3e-64 gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbasp... 234 1e-62 gi|300310466|ref|YP_003774558.1| dipeptide/oligopeptide/nickel A... 81 2e-16 >gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 1085 bits (2805), Expect = 0.0, Method: Compositional matrix adjust. Identities = 524/524 (100%), Positives = 524/524 (100%) Query: 1 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK 60 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK Sbjct: 9 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK 68 Query: 61 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDG 120 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDG Sbjct: 69 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDG 128 Query: 121 YTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI 180 YTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI Sbjct: 129 YTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI 188 Query: 181 NTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQAR 240 NTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQAR Sbjct: 189 NTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQAR 248 Query: 241 AQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNPFIR 300 AQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNPFIR Sbjct: 249 AQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNPFIR 308 Query: 301 FEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDV 360 FEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDV Sbjct: 309 FEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDV 368 Query: 361 TGLHPDGGLVKWAENANVGYMPQDPTEEFAGGETLTDWMGKWTQEGDDDQAVRGILGRLL 420 TGLHPDGGLVKWAENANVGYMPQDPTEEFAGGETLTDWMGKWTQEGDDDQAVRGILGRLL Sbjct: 369 TGLHPDGGLVKWAENANVGYMPQDPTEEFAGGETLTDWMGKWTQEGDDDQAVRGILGRLL 428 Query: 421 FSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDMESIESLNIALEKYAG 480 FSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDMESIESLNIALEKYAG Sbjct: 429 FSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDMESIESLNIALEKYAG 488 Query: 481 TLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDYLASQGIE 524 TLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDYLASQGIE Sbjct: 489 TLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDYLASQGIE 532 >gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 301 bits (770), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 182/533 (34%), Positives = 280/533 (52%), Gaps = 28/533 (5%) Query: 1 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK 60 +Q G KPL EN+ + G++ GLIGANG GKS+ +L G+L GN+ R+ Sbjct: 9 LQRGVKPLLENVDLTLNPGDKIGLIGANGAGKSSLFAMLRGELHADQGNIDFPARWRMAY 68 Query: 61 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDG 120 + Q+ A + ++ + G + +E A PD D A+L A+ D Sbjct: 69 VAQETPALDR-PAIEYAIDGDAHLRQLEAELARAEAQPDDKHDGN-HIAELHTALADADA 126 Query: 121 YTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDI 180 YT +RA +LLLG+G +EQ P+++ + GW++R+ LAQAL D+LLLDEPTN+LD+ Sbjct: 127 YTVRSRAEQLLLGLGFSLEQMERPVASFSGGWRMRLNLAQALMCPSDLLLLDEPTNHLDL 186 Query: 181 NTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQAR 240 + I WLE+ L T++IISHDR FL+ V + +D LK Y GNY + + R Sbjct: 187 DAIIWLEDWLKRYEGTLLIISHDRDFLDGVVNVIVHIDDRKLKRYSGNYSAF----ERQR 242 Query: 241 AQQLAANA----KAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIK----------- 285 + QL A K + + A LQ F+ RF A +KARQA SR K + K++ Sbjct: 243 SAQLELMAGMIEKQQRQRAHLQSFIDRFKAKATKARQAQSRMKALSKMEELAPLRAAAEF 302 Query: 286 -VEDIKPSSRVNPFIRFEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGA 344 E +P S NP + E + +E + D+ I ++ G++I ++G Sbjct: 303 SFEFREPLSAPNPLLVMEKVNAGYH--IEGKNGAPDEDKIIVHGIDFSLQNGQRIGLLGV 360 Query: 345 NGAGKTTLLRSIGGDVTGLHPDGGLVKWAENANVGYMPQDPTEEFAGGETLTDWMGKWTQ 404 NGAGK+TL++++ G++ P G + + +GY Q E E+ W Sbjct: 361 NGAGKSTLIKTVAGEID---PLSGTARLGKGLVIGYFAQHQVEMLRHDESPL-WHLTRLA 416 Query: 405 EGDDDQAVRGILGRLLFSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMD 464 +Q +R LG F+G S+ SGGEK R+ ++ R N+LL+DEPTNH+D Sbjct: 417 PDVREQELRNFLGSFNFNGTMATSSIAPFSGGEKARLALALIVWQRPNLLLLDEPTNHLD 476 Query: 465 MESIESLNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDY 517 +E+ E+L +AL ++ GTL+ VSHDR + + + L V +G++ F G +DY Sbjct: 477 LETREALTMALAQFEGTLVLVSHDRHLLRATTDQFLIVAEGKLQPFDGDLDDY 529 >gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 640 Score = 239 bits (611), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 162/527 (30%), Positives = 278/527 (52%), Gaps = 21/527 (3%) Query: 1 MQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGK 60 + FG L ++ G R GLIG NG GKS+ +KI+ G + G + + N ++ Sbjct: 11 LAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAY 70 Query: 61 LRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYM--KAADLEAKFAEY 118 + Q+ EEM V D V G E+ A ++E +AI +DD + + ++ Sbjct: 71 VDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMHQIQTVLDAA 130 Query: 119 DGYTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNNL 178 D ++ + L + + + G +S G K RV LA L NPD+LLLDEPTN+L Sbjct: 131 DAWSIGNKVETTLDKLNLNRDARIGELSG---GMKKRVALACGLVGNPDVLLLDEPTNHL 187 Query: 179 DINTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASSQ 238 D +I WLE +L + +++ I+HDR FL+ V T + ++D G + +PGN+ Y ++ Sbjct: 188 DFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQTRKAE 247 Query: 239 ARAQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNPF 298 A + NAK + +A+ + ++R+ +++ + R ++++ ++V+ + R Sbjct: 248 LLANEEVENAKFDKFLAQEEVWIRK--GVQARRTRDEGRVRRLEALRVQRSERRERQGQ- 304 Query: 299 IRFE---GEKKLHRLAVETQGITKTY-DRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLR 354 ++ E GE+ ++ E + + K Y + I ++FS ++ G+K+ +IG NGAGKTTLL+ Sbjct: 305 VKLEVGTGERS-GKIVAELENVNKAYGPKTIVRDFSTILMRGDKVGLIGQNGAGKTTLLK 363 Query: 355 SIGGDVTGLHPDGGLVKWAENANVGYMPQDPTEEFAGGETLTDWMGK---WTQEGDDDQA 411 I G+ PD G VK V Y Q + TL D + W + + Sbjct: 364 LILGEEA---PDSGTVKQGTKMQVAYFDQ-MRAQLNEETTLADTIAPGSDWVEVNGQRKH 419 Query: 412 VRGILGRLLFSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDMESIESL 471 V L LF+ + + V+ LSGGE+ R++ +L NVL++DEPTN +D++++E L Sbjct: 420 VMTYLSDFLFAPERARSPVRTLSGGERNRLLLARLFAKPANVLVLDEPTNDLDIDTLELL 479 Query: 472 NIALEKYAGTLIFVSHDREFVSSLATRILEVK-DGQVIDFQGGYEDY 517 LE+Y GT+ VSHDR F+ ++ T+++ + +G ++ GGY D+ Sbjct: 480 EELLEEYEGTVFLVSHDRMFLDNVVTQVIASEGNGVWREYVGGYSDW 526 >gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 234 bits (598), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 181/549 (32%), Positives = 278/549 (50%), Gaps = 66/549 (12%) Query: 6 KPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSLDVNERLGKLRQDQ 65 + + ++IS+ F G + G++G NG GKST +KI+ G + G N +G L Q+ Sbjct: 19 RQILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGEAIPMPNLNIGYLPQEP 78 Query: 66 FAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDY----MKAADLEAKFAEYDGY 121 E V V E++ A ++ DA+YA D D+ + A LEA A DG Sbjct: 79 QLDAEKTVRQEVESALGEVFEAQAKLDAVYAAYAEEDADFDALATEQARLEAIIAAADGN 138 Query: 122 TA----EARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNPDILLLDEPTNN 177 + E A L L P + G +S G K RV L + L S PD+LLLDEPTN+ Sbjct: 139 SLNQQLEMAADALRLP---PWDAKIGVLSG---GEKRRVALCKLLLSKPDMLLLDEPTNH 192 Query: 178 LDINTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASS 237 LD ++ WLE+ L T++ I+HDR+FL+ + ++D G + GNY + L Sbjct: 193 LDAESVDWLEQFLQRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSW-LEQK 251 Query: 238 QARAQQLAANAKAKEK-VAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVN 296 +AR +Q A+ A++K +A+ ++VR+ K RQA S+A+ +R N Sbjct: 252 EARLKQEEASESARQKTIAKELEWVRQ----NPKGRQAKSKARL------------ARFN 295 Query: 297 PFIRFEGEKK-------------LHRLAVETQGITKTY-DRDIFKNFSIMVEAGEKIAII 342 +E +K+ L +E + ++K Y DR + + + + AG + II Sbjct: 296 ELSEYEYQKRNETQEIFIPVAERLGNEVIEFKNVSKGYGDRLLIDDLNFKIPAGAIVGII 355 Query: 343 GANGAGKTTLLRSIGGDVTGLHPDGGLVKWAENANVGYMPQDPT---------EEFAGGE 393 G NGAGK+TL R + G T PD G V V + Q E+ +GG Sbjct: 356 GPNGAGKSTLFRMLAGRET---PDSGEVVLGPTVKVSLVDQSREDLGNKKTVFEDVSGGA 412 Query: 394 TLTDWMGKWTQEGDDDQAVRGILGRLLFSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNV 453 + +G++ + R LGR F G D +K V LSGGE+GR+ K +L NV Sbjct: 413 DILT-VGRF------EMPSRAYLGRFNFKGGDQQKIVGNLSGGERGRLHLAKTLLQGGNV 465 Query: 454 LLMDEPTNHMDMESIESLNIALEKYAGTLIFVSHDREFVSSLATRILEVK-DGQVIDFQG 512 LL+DEP+N +D+E++ +L AL ++AGT++ +SHDR F+ +AT I+ + D QV F G Sbjct: 466 LLLDEPSNDLDVETLRALEDALLEFAGTVLVISHDRWFLDRIATHIIAFEGDSQVTFFDG 525 Query: 513 GYEDYLASQ 521 Y++Y A + Sbjct: 526 NYQEYEADK 534 >gi|300310466|ref|YP_003774558.1| dipeptide/oligopeptide/nickel ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 645 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 120/542 (22%), Positives = 227/542 (41%), Gaps = 95/542 (17%) Query: 8 LFENISVKFGDGNRYGLIGANGCGKSTF-MKILG---GDLEPSAGNVSLDVNERLGKLRQ 63 + +N++ G LIG +G GK+T + +LG + G V R+G+ + Sbjct: 48 IVKNVNFTVARGEVLALIGESGSGKTTIALSLLGYARAGCHIAGGQV------RIGERQI 101 Query: 64 DQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDDYMKAADLEAKFAEYDGYTA 123 DQ + + + M GH A +++ A NP T D + + L+ D +A Sbjct: 102 DQMDEKAL----LAMRGH--HVAYIAQSAAAAFNPARTLMDQVIESALQ--HGVMDKASA 153 Query: 124 EARAGELLLGVGVPIEQHNGPM--SNVAPGWKLRVLLAQALFSNPDILLLDEPTNNLDIN 181 +A EL + +P G V+ G RV+ A AL + P++++LDEPT LD+ Sbjct: 154 MRKAVELFRALALPNPDKIGERYPHQVSGGQLQRVMAAMALVTGPELVILDEPTTALDVT 213 Query: 182 T----IRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYPGNYDDYMLASS 237 T +R + V+ E +T + +SHD + Q+ + + G ++ G+ + A + Sbjct: 214 TQIEVLRAFKNVVKELGTTAVYVSHDLAVVAQMADRIIVLRDGAVR-ETGSTSQVLHAPA 272 Query: 238 QARAQQLAANAKAKEKVAELQDFVRRFSANKSKARQATSRAKQIDKIKVEDIKPSSRVNP 297 + Q L A A +V +L++ + +++A P R++ Sbjct: 273 DSYTQCLLAAASPAARVVQLREILPATLPAEARA-------------------PLLRISG 313 Query: 298 FIRFEGEKKLHRLAVETQGITKTYDRDIFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIG 357 + G RL + G+ I + ++ ++ G + IIG +G+GKTTL R++ Sbjct: 314 LVAGYGAPD--RLGLP--GVR------ILDDINLTIQRGSTVGIIGESGSGKTTLARAVA 363 Query: 358 GDVT----GLHPDGGLVKWA----------------ENANVGYMPQDPTEEFAGGETLTD 397 G + + DG + +NA+ P + G L Sbjct: 364 GMIAPARGSIQLDGETLSPTLEGRTREQFRRVQIVFQNADTALNP-----SHSIGRILNR 418 Query: 398 WMGKWTQEGDDDQAVRGILGRLL----FSGDDVKKSVKVLSGGEKGRMMYGKLMLGRHNV 453 + + G +A+R LL + + LSGG+K R+ + + + ++ Sbjct: 419 PLSFY--HGLKGEAMRRRTAELLDLVQLPATVIDRLPAELSGGQKQRVNLARALAAKPDL 476 Query: 454 LLMDEPTNHMD-------MESIESLNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQ 506 +L DE T+ +D +E + L L+ + +F+SHD V ++ I+ + G+ Sbjct: 477 ILCDEVTSALDTVVAAAILELLAELRRELQV---SYMFISHDISTVRAICDDIVVLYAGR 533 Query: 507 VI 508 ++ Sbjct: 534 MV 535 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 64/233 (27%), Positives = 104/233 (44%), Gaps = 34/233 (14%) Query: 309 RLAVETQGITKTYDRD------IFKNFSIMVEAGEKIAIIGANGAGKTTLLRSIGGDV-T 361 R VE QG+ ++ I KN + V GE +A+IG +G+GKTT+ S+ G Sbjct: 26 RPLVEVQGLRVNARKEDGSQVEIVKNVNFTVARGEVLALIGESGSGKTTIALSLLGYARA 85 Query: 362 GLHPDGGLVKWAENA---------------NVGYMPQDPTEEFAGGETLTDW-MGKWTQE 405 G H GG V+ E +V Y+ Q F TL D + Q Sbjct: 86 GCHIAGGQVRIGERQIDQMDEKALLAMRGHHVAYIAQSAAAAFNPARTLMDQVIESALQH 145 Query: 406 GDDDQAVRGILGRLLFSGDDVKKSVKV-------LSGGEKGRMMYGKLMLGRHNVLLMDE 458 G D+A LF + K+ +SGG+ R+M ++ ++++DE Sbjct: 146 GVMDKASAMRKAVELFRALALPNPDKIGERYPHQVSGGQLQRVMAAMALVTGPELVILDE 205 Query: 459 PTNHMD----MESIESLNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQV 507 PT +D +E + + +++ T ++VSHD V+ +A RI+ ++DG V Sbjct: 206 PTTALDVTTQIEVLRAFKNVVKELGTTAVYVSHDLAVVAQMADRIIVLRDGAV 258 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,619,104 Number of extensions: 69074 Number of successful extensions: 898 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 898 Number of HSP's successfully gapped: 49 Length of query: 524 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 434 Effective length of database: 1,193,720 Effective search space: 518074480 Effective search space used: 518074480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)