BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0067.p (758 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311534|ref|YP_003775626.1| lipoprotein transmembrane prote... 1505 0.0 gi|300310707|ref|YP_003774799.1| esterase of the alpha-beta hydr... 147 4e-36 >gi|300311534|ref|YP_003775626.1| lipoprotein transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 758 Score = 1505 bits (3896), Expect = 0.0, Method: Compositional matrix adjust. Identities = 752/758 (99%), Positives = 753/758 (99%) Query: 1 MHAPCKALLRTLLLALASVSLGGPAVAENSSTAAPAATQPRPRVCLVLSGGGARGYAHLG 60 M APCKALLR LLLALASVSLGGPA AE SSTAAPAATQPRPRVCLVLSGGGARGYAHLG Sbjct: 1 MRAPCKALLRPLLLALASVSLGGPAAAETSSTAAPAATQPRPRVCLVLSGGGARGYAHLG 60 Query: 61 VLQYLEQLHIPIDCIAGTSMGALIGGLYASGLDAGELERRLAATNLSDIAFDRNERSRLP 120 VLQYLEQLHIPIDCIAGTSMGALIGGLYASGLDAGELERRLAATNLSDIAFDRNERSRLP Sbjct: 61 VLQYLEQLHIPIDCIAGTSMGALIGGLYASGLDAGELERRLAATNLSDIAFDRNERSRLP 120 Query: 121 QAQREDEFQYPISISAGLDGSKLKLASGLVQGNKLLALLQNWTAQLPANIDFARLPIPFR 180 QAQREDEFQYPISISAGLDGSKLKLASGLVQGNKLLALLQNWTAQLPANIDFARLPIPFR Sbjct: 121 QAQREDEFQYPISISAGLDGSKLKLASGLVQGNKLLALLQNWTAQLPANIDFARLPIPFR 180 Query: 181 AVATDLSLGSEVVLDHGSLPRAMRASMAVPGLFAPFRIDQRTLVDGGLVSNLPVQTARDM 240 AVATDLSLGSEVVLDHGSLPRAMRASMAVPGLFAPFRIDQRTLVDGGLVSNLPVQTARDM Sbjct: 181 AVATDLSLGSEVVLDHGSLPRAMRASMAVPGLFAPFRIDQRTLVDGGLVSNLPVQTARDM 240 Query: 241 GADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVKAQKALLGAQDILIEPQMEGM 300 GADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVKAQKALLGAQDILIEPQMEGM Sbjct: 241 GADVVIAVNIATPLQDAAQLQSPTAVAQQMVGILIQQNVKAQKALLGAQDILIEPQMEGM 300 Query: 301 SFTDFARGKDGVNAGWEAAQRQQARLLQLSLTPTQWQAYLDRRAHDGMALAAGTRIDAIE 360 SFTDFARGKDGVNAGWEAAQRQQARLLQLSLTPTQWQAYLDRRAHDGMALAAGTRIDAIE Sbjct: 301 SFTDFARGKDGVNAGWEAAQRQQARLLQLSLTPTQWQAYLDRRAHDGMALAAGTRIDAIE 360 Query: 361 IRSGSRIPASYVRSRLAVKEGDVYDGQRINQQLSQLSTNGDFNNVTQELVSREDGRHVLL 420 IRSGSRIPASYVRSRLAVKEGDVYDGQRINQQLSQLSTNGDFNNVTQELVSREDGRHVLL Sbjct: 361 IRSGSRIPASYVRSRLAVKEGDVYDGQRINQQLSQLSTNGDFNNVTQELVSREDGRHVLL 420 Query: 421 IDAEEKSWGPQYLLFGLGVSNSFNGRGGFNLQLGHRYPWMNQSGLEWRNDLVLGAKRASL 480 IDAEEKSWGPQYLLFGLGVSNSFNGRGGFNLQLGHRYPWMNQSGLEWRNDLVLGAKRASL Sbjct: 421 IDAEEKSWGPQYLLFGLGVSNSFNGRGGFNLQLGHRYPWMNQSGLEWRNDLVLGAKRASL 480 Query: 481 QTELRQPIWGSTGAYLSPYLEVSRRYVDLYPDGSDVKASPINEYRIDSAQGGLDLGLPLS 540 QTELRQPIWGSTGAYLSPYLEVSRRYVDLYPDGSDVKASPINEYRIDSAQGGLDLGLPLS Sbjct: 481 QTELRQPIWGSTGAYLSPYLEVSRRYVDLYPDGSDVKASPINEYRIDSAQGGLDLGLPLS 540 Query: 541 RLGELRLGLSYQHVNYSPTYNLAAGGGLLFESVQTSQPVARLRLTIDQLDDALFPRKGYY 600 RLGELRLGLSYQHVNYSPTYNL AGGGLLFESVQTSQPVARLRLTIDQLDDALFPRKGYY Sbjct: 541 RLGELRLGLSYQHVNYSPTYNLPAGGGLLFESVQTSQPVARLRLTIDQLDDALFPRKGYY 600 Query: 601 LSAEANRGLGSDEHRFSNVQARTLWAVSRERNTLNVALEGASSLSANSAGIGFTLGGFQH 660 LSAEANRGLGSDEHRFSNVQARTLWAVSRERNTLNVALEGASSLSANSAGIGFTLGGFQH Sbjct: 601 LSAEANRGLGSDEHRFSNVQARTLWAVSRERNTLNVALEGASSLSANSAGIGFTLGGFQH 660 Query: 661 LSAYAPDQFFGNYLLYGRLSWLRDLVDTSLPGLRNPVLGASLEAGNVWQSRDAFGDGPYK 720 LSAYAPDQFFGNYLLYGRLSWLRDLVDTSLPGLRNPVLGASLEAGNVWQ+RDAFGDGPYK Sbjct: 661 LSAYAPDQFFGNYLLYGRLSWLRDLVDTSLPGLRNPVLGASLEAGNVWQNRDAFGDGPYK 720 Query: 721 KSLSLFLGGSSPVGPLYFGVALGQQGVWNLYLQLGRVF 758 KSLSLFLGGSSPVGPLYFGVALGQQGVWNLYLQLGRVF Sbjct: 721 KSLSLFLGGSSPVGPLYFGVALGQQGVWNLYLQLGRVF 758 >gi|300310707|ref|YP_003774799.1| esterase of the alpha-beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 147 bits (370), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 102/292 (34%), Positives = 151/292 (51%), Gaps = 34/292 (11%) Query: 29 NSSTAAPAATQPRP-RVCLVLSGGGARGYAHLGVLQYLEQLHIPIDCIAGTSMGALIGGL 87 SST P + PR ++ L L GG ARG+AH+GV++ LE + +D + GTS G+L+G L Sbjct: 40 TSSTVTPVSPTPRRLKIGLALGGGAARGFAHIGVIKALEAQGVQVDVVTGTSAGSLVGAL 99 Query: 88 YASGLDAGELERRLAATNLSDIAFDRNERSRLPQAQREDEFQYPISISAGLDGSKLKLAS 147 YA+G D L++ A + + I+ ++ P+ S Sbjct: 100 YAAGNDGFALQKLALAMDEAAIS----------------DWSVPL----------FSKVS 133 Query: 148 GLVQGNKLLALLQNWTAQLPANIDFARLPIPFRAVATDLSLGSEVVLDHGSLPRAMRASM 207 G+++G + QN+ + N+ + IPF AVATDL G ++ G+ A+RAS Sbjct: 134 GVLKGEGV----QNYVNKALNNVPIEKFKIPFGAVATDLRSGQPILFQRGNAGIAVRASS 189 Query: 208 AVPGLFAPFRIDQRTLVDGGLVSNLPVQTARDMGADVVIAVNIATPLQDAAQLQSPTAVA 267 AVPG+F +I T VDGGLVS +PV+ AR MGAD VIAVNI+ + A S V Sbjct: 190 AVPGVFQSVKIGDHTYVDGGLVSPVPVRFARQMGADFVIAVNISANPEAQAASSSSVDVL 249 Query: 268 QQMVGILIQQNVKAQKALLGAQDILIEPQMEGMSFTDFARGKDGVNAGWEAA 319 Q I+ Q + + L DI+++P + M +DFA + AG +AA Sbjct: 250 LQTFAIMGQTINQYE---LKNADIVLQPGLGAMKGSDFASRNVAIMAGEQAA 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,320,029 Number of extensions: 99061 Number of successful extensions: 307 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 305 Number of HSP's successfully gapped: 2 Length of query: 758 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 665 Effective length of database: 1,179,515 Effective search space: 784377475 Effective search space used: 784377475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)