BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0058.p (553 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311543|ref|YP_003775635.1| major facilitator superfamily p... 1113 0.0 gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 194 2e-50 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 184 1e-47 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 183 3e-47 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 179 7e-46 >gi|300311543|ref|YP_003775635.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 553 Score = 1113 bits (2878), Expect = 0.0, Method: Compositional matrix adjust. Identities = 553/553 (100%), Positives = 553/553 (100%) Query: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI Sbjct: 1 MTTASVNTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFI 60 Query: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA Sbjct: 61 FALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITA 120 Query: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV Sbjct: 121 PIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGV 180 Query: 181 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG Sbjct: 181 RTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFG 240 Query: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF 300 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF Sbjct: 241 QWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFF 300 Query: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC 360 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC Sbjct: 301 LFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAALQKSPVIVTADPASC 360 Query: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK 420 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK Sbjct: 361 QFQFNPTGTKKFTSSCDIVKAKLSAASVNYQNVAGAPGSVATVKIGDRIITSYDAAGLSK 420 Query: 421 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV 480 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV Sbjct: 421 EDAAAKDKQLSKDLADDIKAAGYPSKADPEQMNKPMVLLLLFILVIYVTMVYGPIAACLV 480 Query: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM 540 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM Sbjct: 481 EMFPTRIRYTSMSMPYHIGNGWFGGLLPTTSFALVAFKGDIYYGLWYPIVIAMITFVIGM 540 Query: 541 LFVKETVRNNIHD 553 LFVKETVRNNIHD Sbjct: 541 LFVKETVRNNIHD 553 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 194 bits (493), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 125/349 (35%), Positives = 194/349 (55%), Gaps = 16/349 (4%) Query: 6 VNTTARGITP----EEKKVIFA---SSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTA 58 ++ T + TP E +K IFA +S G + EW+DFY+Y A A FF DP + Sbjct: 1 MSDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQ 60 Query: 59 FIFALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGI 118 + FAAGF++RP G +FGR+ D GRK + L+++L+M + V V+P Y +IG Sbjct: 61 LLNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGA 120 Query: 119 TAPIILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVIL 178 AP +L++ R+ QGL++GGEYG +ATY++E AP GRRG F ++ T G L++LV+ Sbjct: 121 WAPALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF 180 Query: 179 GVRTAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNE-SPAFLKMKAEGKTSKAPLTE 237 G++ + +A WGWR+PF++ + +++++R S+ E S A + K + T K L Sbjct: 181 GMQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH 240 Query: 238 AFGQWKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLAT 297 K L +VG TAG ++++YT Y +L T +D AN ++ AL + Sbjct: 241 --------KRAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYM 292 Query: 298 PFFLFFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTHFANPALEAAL 346 FG++SDKIGR+ +L V FPI L ++P + AL Sbjct: 293 ILQPIFGAISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL 341 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 113/330 (34%), Positives = 181/330 (54%), Gaps = 16/330 (4%) Query: 7 NTTARGITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQ-FFAGTDPNTAFIFALLA 65 +T + + KK + +G+ E+YDF++Y + A++I Q FF DP TA I +L Sbjct: 3 STQSNAHHQQSKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLAT 62 Query: 66 FAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILI 125 + G++ RP GA V G LGD GRK L + +MG ST VG+LP Y+ +G+ AP +L+ Sbjct: 63 YGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLV 122 Query: 126 ILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVG 185 I+R+ QG A+ GE GA++ + EH+P GRRG F+++ G L+ V L + + Sbjct: 123 IMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMP 182 Query: 186 EAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQW--K 243 F WGWRIPFL+S L++ IR +++E+P F E ++P+ +AF Sbjct: 183 AEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPN 242 Query: 244 NLKLVILALVGLTAGQAVVWYTGQFYAL-----FFLTQTLKVDGPTANILIAAALLLATP 298 L++V +AL+ + A ++ G YA+ + + + P ++A +L P Sbjct: 243 MLRVVCMALMNVIPVVATIF--GAAYAVQPAYGIGFAKDVYLWIPVVGNIVA---VLVIP 297 Query: 299 FFLFFGSLSDKIGRKPIILGGCLIAAVTYF 328 + G LSD+IGR+P I+ G L+A + F Sbjct: 298 ---YVGKLSDRIGRRPPIIVGSLLAGLFAF 324 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 183 bits (464), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 108/338 (31%), Positives = 179/338 (52%), Gaps = 17/338 (5%) Query: 5 SVNTTARG---ITPEEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIF 61 S NT A G P+ ++ I +SS+G EW+D +YG+ A +IAKQFF D + + + Sbjct: 2 SSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLL 61 Query: 62 ALLAFAAGFIVRPFGALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAP 121 F F +RP GA+V G D GRK +++I++M T ++ +P+Y SIG+ AP Sbjct: 62 TFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAP 121 Query: 122 IILIILRILQGLALGGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVR 181 + + +++QG + GGE+G + ++ EHAP RRG F++W + + L L+ L + Sbjct: 122 AGIALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 182 TAVGEAAFADWGWRIPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQ 241 + A WGWR+PF+ +L+ ++IR ++E+P F K + + APL + F Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF---KESSEKTNAPLRDTFAH 237 Query: 242 WKNLKLVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGPTANILIAAALLLATPFFL 301 K ++L+I AG ++ + +L+ T +K G A AA + F+ Sbjct: 238 QK-MRLLI------GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFI 290 Query: 302 ---FFGSLSDKIGRKPIILGGCLIAAVTYFPIFSGLTH 336 G+LSDKIGR P ++ + V +P+F LT+ Sbjct: 291 GSPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTN 328 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 179 bits (453), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 105/324 (32%), Positives = 177/324 (54%), Gaps = 20/324 (6%) Query: 16 EEKKVIFASSLGTVFEWYDFYLYGSLAAIIAKQFFAGTDPNTAFIFALLAFAAGFIVRPF 75 + ++ I A+ +G EW+DF +Y A IIAK FF + T+ + A+ F GF +RP Sbjct: 18 QRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 77 Query: 76 GALVFGRLGDLVGRKYTFLVTILIMGASTFIVGVLPNYNSIGITAPIILIILRILQGLAL 135 G ++ G D VGRK VTIL+M T ++G+ P Y SIG+ AP+I+++ R+LQG + Sbjct: 78 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSA 137 Query: 136 GGEYGGAATYVAEHAPEGRRGSFTAWIQTTATMGLFLSLLVILGVRTAVGEAAFADWGWR 195 GGE G A ++ E+APE R +++WIQ + + + L V V ++ AA A WGWR Sbjct: 138 GGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWR 197 Query: 196 IPFLVSILLLAISVWIRMSMNESPAFLKMKAEGKTSKAPLTEAFGQWKNLKLVILALVGL 255 +PFL+ I++ + +IR + E+P FL+ E + +PL E + ++V Sbjct: 198 LPFLLGIIIGPVGYYIRHHLEETPTFLE---EKDKTDSPLKEVIRNFPRETSASFSMV-- 252 Query: 256 TAGQAVVWYTGQFYALFFL-TQTLKV------DGPTANILIAAALLLATPFFLFFGSLSD 308 ++W + LF++ T ++KV G A ++ +++ P G L+D Sbjct: 253 -----ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAP---IVGRLAD 304 Query: 309 KIGRKPIILGGCLIAAVTYFPIFS 332 +IGR+P++ G + + +P+F+ Sbjct: 305 RIGRRPLLSGSAALILMLAYPMFA 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,457,727 Number of extensions: 52499 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 17 Length of query: 553 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 463 Effective length of database: 1,193,720 Effective search space: 552692360 Effective search space used: 552692360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)