BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0055.p (584 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311546|ref|YP_003775638.1| Fe3+ ABC transporter permease [... 1146 0.0 gi|300309934|ref|YP_003774026.1| sulfate ABC transporter permeas... 78 2e-15 gi|300313815|ref|YP_003777907.1| Fe3+ ABC transporter permease [... 70 4e-13 gi|300310985|ref|YP_003775077.1| sulfate ABC transporter permeas... 65 2e-11 gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC trans... 59 2e-09 >gi|300311546|ref|YP_003775638.1| Fe3+ ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 578 Score = 1146 bits (2964), Expect = 0.0, Method: Compositional matrix adjust. Identities = 576/578 (99%), Positives = 576/578 (99%) Query: 7 MPATVSAPLPTLSAETSTSTRPASVPDRSQWLPGLLKWLLLMLLALGLVGPLLFILAQAV 66 MPATVSAPLPTLSAETSTSTRPASVPDRSQWLP LLKWLLLMLLALGLVGPLLFILAQAV Sbjct: 1 MPATVSAPLPTLSAETSTSTRPASVPDRSQWLPALLKWLLLMLLALGLVGPLLFILAQAV 60 Query: 67 LTPAGEWAGSRPFAQLFANINFLPMLGRSLWVSAATAVVTVPLAYVFAYALQRTCIPGKG 126 LTPAGEWAGSRPFAQLFANINFLPMLGRSLWVSA TAVVTVPLAYVFAYALQRTCIPGKG Sbjct: 61 LTPAGEWAGSRPFAQLFANINFLPMLGRSLWVSAMTAVVTVPLAYVFAYALQRTCIPGKG 120 Query: 127 LWRGIGLLPLLAPSLLPGIALIYLFGNQGLFKEWMDGSIYGFWGILLGEVFYTFPHALMI 186 LWRGIGLLPLLAPSLLPGIALIYLFGNQGLFKEWMDGSIYGFWGILLGEVFYTFPHALMI Sbjct: 121 LWRGIGLLPLLAPSLLPGIALIYLFGNQGLFKEWMDGSIYGFWGILLGEVFYTFPHALMI 180 Query: 187 LLSALSQADGRLYDAAQAMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGVPKIT 246 LLSALSQADGRLYDAAQAMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGVPKIT Sbjct: 181 LLSALSQADGRLYDAAQAMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGVPKIT 240 Query: 247 GGAYSVLAVEAYKAVVGQQQFGRGALIGLLLLVPALLTFTLDAWLQRRQRASMSSRSETY 306 GGAYSVLAVEAYKAVVGQQQFGRGALIGLLLLVPALLTFTLDAWLQRRQRASMSSRSETY Sbjct: 241 GGAYSVLAVEAYKAVVGQQQFGRGALIGLLLLVPALLTFTLDAWLQRRQRASMSSRSETY 300 Query: 307 VPQPDWRRDGANLLAVALIASAILMMIVVAMGASLVKLWPYNLQLTLQHYDFDNMDGGGW 366 VPQPDWRRDGANLLAVALIASAILMMIVVAMGASLVKLWPYNLQLTLQHYDFDNMDGGGW Sbjct: 301 VPQPDWRRDGANLLAVALIASAILMMIVVAMGASLVKLWPYNLQLTLQHYDFDNMDGGGW 360 Query: 367 LAYWNSLRLACGTSVAGTLLIFCGAWLMEKTRGQAWLSTLLRMLCFLPMAVPGLVLGLGS 426 LAYWNSLRLACGTSVAGTLLIFCGAWLMEKTRGQAWLSTLLRMLCFLPMAVPGLVLGLGS Sbjct: 361 LAYWNSLRLACGTSVAGTLLIFCGAWLMEKTRGQAWLSTLLRMLCFLPMAVPGLVLGLGS 420 Query: 427 LLFFNHPANPFNFLYGTMTLLVLCCVVHFYTTAQLTAVTALKQLDPEFEAASLSLKVPLL 486 LLFFNHPANPFNFLYGTMTLLVLCCVVHFYTTAQLTAVTALKQLDPEFEAASLSLKVPLL Sbjct: 421 LLFFNHPANPFNFLYGTMTLLVLCCVVHFYTTAQLTAVTALKQLDPEFEAASLSLKVPLL 480 Query: 487 KTFIRVTVPLCLPQLLEIFRYLFVSSMTTVSAVIFLYRPDTVLASVAVLNMDDAGDVGPA 546 KTFIRVTVPLCLPQLLEIFRYLFVSSMTTVSAVIFLYRPDTVLASVAVLNMDDAGDVGPA Sbjct: 481 KTFIRVTVPLCLPQLLEIFRYLFVSSMTTVSAVIFLYRPDTVLASVAVLNMDDAGDVGPA 540 Query: 547 AAMSTLILLTSAAASLLMHLGGRRWLARAQAWRRGRAH 584 AAMSTLILLTSAAASLLMHLGGRRWLARAQAWRRGRAH Sbjct: 541 AAMSTLILLTSAAASLLMHLGGRRWLARAQAWRRGRAH 578 >gi|300309934|ref|YP_003774026.1| sulfate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 291 Score = 78.2 bits (191), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 67/262 (25%), Positives = 112/262 (42%), Gaps = 8/262 (3%) Query: 24 TSTRPASVPDRSQWLPGLLKWLLLMLLALGLVGPL-LFILAQAVLTPAGEWAGSRPFAQL 82 TS RPA P G WL + L L L +A+ V A G+ + + Sbjct: 7 TSARPARPPASRPRRAGNQVWLCFAVPGLTLFCAFWLLPMARLVGVGASGPDGAMAYLSV 66 Query: 83 FANINFLPMLGRSLWVSAATAVVTVPLAYVFAYALQRTCIPGKGLWRGIGLLPLLAPSLL 142 N ++ L +L +SAA T+ ++ LQR PG+ L + PL P ++ Sbjct: 67 LTNRHYFASLVSTLALSAAVTAATLAISVFVGLFLQRHRFPGQALLLAMLTFPLAFPGVV 126 Query: 143 PGIALIYLFGNQGLFKEWMDGSI-------YGFWGILLGEVFYTFPHALMILLSALSQAD 195 G +I L G QGL +G Y G+ LG ++++ P ++ +++A + D Sbjct: 127 VGFMVIMLAGRQGLIGALTNGLFGESLVFAYSLAGLFLGYLYFSIPRVILTVVAAAEKLD 186 Query: 196 GRLYDAAQAMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGVPKITGGAYSVLAV 255 L +AA+++GA W++ V LPA + + + F ++ FG VL + Sbjct: 187 PSLEEAARSLGARPWQVLLHVVLPALLPALVSSGAICFATSVGAFGTAFTLAANIDVLPM 246 Query: 256 EAYKAVVGQQQFGRGALIGLLL 277 Y G F A + ++L Sbjct: 247 TIYNEFTGYANFATAASLSIVL 268 >gi|300313815|ref|YP_003777907.1| Fe3+ ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 603 Score = 70.1 bits (170), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 136/587 (23%), Positives = 234/587 (39%), Gaps = 78/587 (13%) Query: 30 SVPDRSQWLPGL---LKW---LLLMLLALGLVGPLLFILAQAVLT-----PAGEWAGSRP 78 ++P Q LP L W L+++L AL + PLL I Q+ L+ P E Sbjct: 6 TLPRSEQALPARRFRLNWPRGLVVLLAALAIFVPLLLIFYQSFLSAPFFAPVKELTFDS- 64 Query: 79 FAQLFANINFLPMLGRSLWVSAATAVVTVPLAYVFAYALQRTCIPGKGLWRGIGLLPLLA 138 + +F + +F L ++ A++ VPL + A+ + RT +PG+ ++P+ Sbjct: 65 YRFIFDDPDFRQAFVNGLTLATGLALIAVPLGGMLAFLMVRTDLPGRSFIAPALMVPIFV 124 Query: 139 PSLLPGIALIYLFGNQGLFKEWMDG------------SIYGFWGILLGEVFYTFPHALMI 186 ++ G + G G + W+ +IY F I++ PH + Sbjct: 125 SPMVIGFGYVVSMGPVGFYTVWVKNLLSVFGFEGDPWNIYSFTSIVIIAGLTHVPHVYLY 184 Query: 187 LLSALSQADGRLYDAAQAMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGVPKIT 246 +AL + +AA+ GAS ++ V+LP + + LVF L FG+ + Sbjct: 185 ASAALRSLGSDVEEAARVAGASPLQVALNVSLPMIMPALAYSGVLVFFLGFEVFGLVLVL 244 Query: 247 GG--AYSVLAVEAYKAV--VGQQQFGRGALIGLLLLVPALLTFTLDAWLQRRQRASMSSR 302 G + VL YK +G + A + + L+ + L WL R ++ + Sbjct: 245 GDPEGHLVLPTYLYKLTNKLGTPSYHLMAAVAVCLVAVTMPLVMLQRWLLRSANKYVAIK 304 Query: 303 S----ETYVPQPDWRRDGANLLAVALIASAILMMIVVAMGA----SLVKLWPYNLQLTLQ 354 + +P WR +A LIA +L +++ + + V W + L L+ Sbjct: 305 GKGARQKALPLGKWR-----WVAFVLIAGWLLFTVIIPLSGIGLRTFVSHWGDGVSL-LE 358 Query: 355 HYDFDNMDGGGWLAYWNSLRLACGT-------SVAGTLLIFC-GAWLMEKTRGQAWLSTL 406 F+N+ L W+ L G + G L + C A + R Q ++ Sbjct: 359 VLTFENL-----LNVWDQPSLVRGIINTVLIGVIGGALAVACYTAIALAMHRKQDGVTRF 413 Query: 407 LRMLCFLPMAVPGLVLGLGSL-LFFNHPANPFNFLYGTMTLLVLCCVVHF---------- 455 L +P AVPGL+ GL L +F P+ + L G + L V F Sbjct: 414 LDYSVLVPRAVPGLLAGLSFLWVFLFVPSLLDSALKGIDNPIALWLVDSFIPALRGLRST 473 Query: 456 -------YTTAQLT-----AVTALKQLDPEFEAASLSLKVPLLKTFIRVTVPLCLPQLLE 503 Y+ + TAL Q+ PE E A+ ++ + VT+PL L+ Sbjct: 474 IFSVWLAYSVVWMAYGLRLINTALLQVGPELEEAARAVGASRSRVTRDVTLPLVRYGLVG 533 Query: 504 IFRYLFVSSMTTVSAVIFLYRPDTVLASVAVLNMDDAGDVGPAAAMS 550 + +F+ S ++L P T + +++M AG V AA+S Sbjct: 534 AWLMVFLIFEREYSTGVYLLSPGTEVIGSMIVSMWAAGAVELVAALS 580 >gi|300310985|ref|YP_003775077.1| sulfate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 46/155 (29%), Positives = 71/155 (45%), Gaps = 8/155 (5%) Query: 102 TAVVTVPLAYVF----AYALQRTCIPGKGLWRGIGLLPLLAPSLLPGIALIYLFGNQGLF 157 TA + VPL VF A+A+ + GK L + LP ++ G+ + LFG QG Sbjct: 85 TAAIAVPLNLVFGVAAAWAIAKFEFRGKNLLLTLIDLPFSVSPVISGLIYVLLFGLQGYL 144 Query: 158 KEWMDGS----IYGFWGILLGEVFYTFPHALMILLSALSQADGRLYDAAQAMGASRWRIF 213 W+ ++ GI+L +F TFP L+ + +AA +GAS WR F Sbjct: 145 GPWLREHDIKILFAVPGIVLATIFVTFPFVARELIPLMQSQGSEEEEAALVLGASGWRTF 204 Query: 214 TTVTLPATRYGIFGALCLVFTLTITDFGVPKITGG 248 VTLP ++G+ + L + +FG + G Sbjct: 205 WHVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSG 239 >gi|300309935|ref|YP_003774027.1| spermidine/putrescine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 46/159 (28%), Positives = 75/159 (47%), Gaps = 11/159 (6%) Query: 88 FLPMLGRSLWVSAATAVVTVPLAYVFAYALQRTCIPGKGLWRGIG---LLPLLAPSLLPG 144 + P + RSL+++ A VT+ AY L R L R I ++P+ P L Sbjct: 57 YQPTIWRSLFIALACLAVTLAAGVPLAYMLARYRGGNSRLARVIEELLMMPVAVPGLATA 116 Query: 145 IALIYLFGNQGLFKEWMDGSIYGFWGILLGEVFYTFPHALMILLSALSQAD-GRLYDAAQ 203 +ALI +G F+ W IL+G V +T P + +L+ + + +L +AA Sbjct: 117 LALIMAYGQYREFRA-------SLWFILVGHVIFTLPFMVRPVLAVMQSSQLAQLEEAAA 169 Query: 204 AMGASRWRIFTTVTLPATRYGIFGALCLVFTLTITDFGV 242 ++GA R R F + +P GI +V TL+I +F + Sbjct: 170 SLGAGRMRRFFGIVIPNAASGILAGALMVVTLSIGEFNI 208 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,428,430 Number of extensions: 48829 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 350 Number of HSP's successfully gapped: 12 Length of query: 584 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 494 Effective length of database: 1,193,720 Effective search space: 589697680 Effective search space used: 589697680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)