BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0054.p (369 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbasp... 725 0.0 gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transport... 260 1e-70 gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC trans... 242 3e-65 gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC trans... 234 8e-63 gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC trans... 232 3e-62 >gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/359 (100%), Positives = 359/359 (100%) Query: 11 MTAATEEDAAVFLSVREVEKRFGSFTALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAG 70 MTAATEEDAAVFLSVREVEKRFGSFTALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAG Sbjct: 1 MTAATEEDAAVFLSVREVEKRFGSFTALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAG 60 Query: 71 LERQDSGRLHADGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRER 130 LERQDSGRLHADGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRER Sbjct: 61 LERQDSGRLHADGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRER 120 Query: 131 VTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQ 190 VTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQ Sbjct: 121 VTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQ 180 Query: 191 LEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADF 250 LEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADF Sbjct: 181 LEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADF 240 Query: 251 IGQANWLPFTRLSGSQVAVGGLHLEIQPDESDRASGRLFCRPEAVQLFSDESCANRFLAR 310 IGQANWLPFTRLSGSQVAVGGLHLEIQPDESDRASGRLFCRPEAVQLFSDESCANRFLAR Sbjct: 241 IGQANWLPFTRLSGSQVAVGGLHLEIQPDESDRASGRLFCRPEAVQLFSDESCANRFLAR 300 Query: 311 VVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLPRQSLQVFA 369 VVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLPRQSLQVFA Sbjct: 301 VVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLPRQSLQVFA 359 >gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 260 bits (665), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 155/354 (43%), Positives = 214/354 (60%), Gaps = 12/354 (3%) Query: 23 LSVREVEKRFGSFTALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAGLERQDSGRLHAD 82 + V ++KRFGSFTAL+ VSL GE+ LLGPSGCGKTTLLR IAGLE DSG++ D Sbjct: 3 IEVHHIQKRFGSFTALNDVSLNFPAGELTALLGPSGCGKTTLLRIIAGLESPDSGQVLLD 62 Query: 83 GRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQK------RERVTEMLS 136 G D S + R G +FQ YALF +++V +N+A+GL R Q+ RE+V +L Sbjct: 63 GEDASARHVRERQVGFVFQHYALFKHMTVFENIAFGLRVKPRSQRPSEAVIREKVKRLLE 122 Query: 137 MVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQLEIRRL 196 +V LD D+YP QLSGGQ+QR+ALARALA P +LLLDEP ALDA+VR+ L+ +RRL Sbjct: 123 LVQLDWLADRYPPQLSGGQRQRIALARALAVEPRVLLLDEPFGALDAKVRKELRRWLRRL 182 Query: 197 QKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADFIGQANW 256 + +T++ VTHDQEEA+ +AD++ +M G++EQ GTPE +Y PA+PFV F+G N Sbjct: 183 HDELHVTSIFVTHDQEEALEVADQVVLMNKGQVEQIGTPEAVYNHPASPFVYGFLGNVN- 241 Query: 257 LPFTRLSGSQVAVGGLHLEIQPDESDR-ASGRLFCRPEAVQLFSDESCANRFLARVVDRI 315 L RL + GG E ++ R A+G F RP + + A + ++ Sbjct: 242 LFHGRLHEGVLDAGGARFEAPGHDNIRDAAGVGFVRPHDLDVERYSPGAEGIVVQLRRAH 301 Query: 316 YLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLPRQSLQVFA 369 +G +L L D L+ V +ER A L ++G + + + LQVFA Sbjct: 302 AIGPLAQLELERD--DNAELIEAVIP--NERFAQLGLKNGETLVVRPRRLQVFA 351 >gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 242 bits (618), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 137/315 (43%), Positives = 199/315 (63%), Gaps = 6/315 (1%) Query: 20 AVFLSVREVEKRFGSFT-ALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAGLERQD-SG 77 AV + + + K F + T AL + L++ GE + LLGPSGCGKTT LR IAGLE D G Sbjct: 7 AVSIRLSQCAKSFANGTRALQPLDLQIHPGETLVLLGPSGCGKTTTLRMIAGLEFPDEGG 66 Query: 78 RLHADGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLG--RMSRQQKRERVTEML 135 R+ D++ LP + R G++FQ+YALFPN+SV +N+AYG+ +++ ++ ERV +L Sbjct: 67 RVLFGEEDVTMLPIEKRGVGMVFQNYALFPNMSVGENIAYGMKIRKIAAGERAERVERLL 126 Query: 136 SMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQLEIRR 195 MV L G + QLSGGQ+QRVALARALA P +LLLDEPL+ALDA++RE ++ ++ + Sbjct: 127 EMVHLQGLSHRRVDQLSGGQKQRVALARALAMEPRVLLLDEPLTALDAKLREAVRSDLNK 186 Query: 196 LQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADFIGQAN 255 L + IT + VTHDQ EAM + DRI VM+ G+I Q GTP++IY PA FVA+FIG N Sbjct: 187 LLRSLGITAIYVTHDQGEAMALGDRIVVMERGKISQIGTPQEIYYHPANDFVAEFIGAMN 246 Query: 256 WLPFTRLSGSQVAVGGLHLEIQPDESDRASGRLFCRPEAVQLFS-DESCANRFLARVVDR 314 + ++G ++ + L + PD + RPE V++ E+ A+ ++++ Sbjct: 247 RVDGI-VAGDRLQLPTGTLPLPPDAVAGQPAQALFRPEDVEVIDITEADAHCLRGKLINT 305 Query: 315 IYLGDRYRLALAADA 329 +LGDR R + A Sbjct: 306 FFLGDRTRFEIEIGA 320 >gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 362 Score = 234 bits (597), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 153/367 (41%), Positives = 217/367 (59%), Gaps = 29/367 (7%) Query: 23 LSVREVEKRFGSFT----ALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAGLERQDSGR 78 LSV ++ +G+ L VS+E++RGE+V LLGPSG GKTTLLR +AGLE +G Sbjct: 4 LSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGA 63 Query: 79 LHA------DGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRE--- 129 + DG ++P + R+ G++FQSYAL+P+ +VADNVAYGL ++ + E Sbjct: 64 IDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGL-KLRKTPSGEIAT 122 Query: 130 RVTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHL 189 RV +LS +GL ++YP QLSGGQQQRVA+ARAL +P ++LLDEPLS LDA++RE Sbjct: 123 RVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEA 182 Query: 190 QLEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVAD 249 + +R L + ++ LMVTHDQ EAM ++DRI ++ +GRIEQ GTP+ +Y+ P T F A+ Sbjct: 183 RAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAE 242 Query: 250 FIGQANWLPFTRLS--GSQVAV---GGLHLEIQPDESDRASGRLFC---RPEAVQLFSDE 301 F+G N L LS G +V V GG + SD G R E V++ S E Sbjct: 243 FMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRI-SGE 301 Query: 302 SCANRFLARVVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLP 361 + N + +YLGDR+ D P ++ + + A +R+ Q W++LP Sbjct: 302 ASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSV--GLRAYAPQRL----DQGEYWLSLP 355 Query: 362 RQSLQVF 368 QSL F Sbjct: 356 EQSLWAF 362 >gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 232 bits (592), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 135/336 (40%), Positives = 192/336 (57%), Gaps = 24/336 (7%) Query: 7 AAPFMTAATEED---AAVFLSVREVEKRF-GSFTALDRVSLEVRRGEMVCLLGPSGCGKT 62 AA M A +E + V + V+K + G + + L++RRGE + LLGPSG GKT Sbjct: 3 AAYAMKQAAQESVQQSDVLVQFCGVKKTYDGENLIVKNLDLDIRRGEFLTLLGPSGSGKT 62 Query: 63 TLLRTIAGLERQDSGRLHADGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLG-- 120 T L +AG E G + DG+ ++++PP R+ G++FQ+YALFP++++A NVAY L Sbjct: 63 TCLMMLAGFEFPTGGEIRLDGQLLNRVPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVR 122 Query: 121 RMSRQQKRERVTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSA 180 +M R + +V + L MV + D+YP QLSGGQQQRVALAR L P L+L+DEPL A Sbjct: 123 KMDRATREAKVKQALDMVQMGKFGDRYPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGA 182 Query: 181 LDAQVREHLQLEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYR 240 LD Q+REH+QLE++ L K+ +T + VTHDQ EA+ M+DR+AV G I+Q ++Y Sbjct: 183 LDKQLREHMQLELKALHKRLGVTFVYVTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYE 242 Query: 241 RPATPFVADFIGQANWLPFTRLSGSQVAVGGLHLEIQPDESDRASG------------RL 288 P FVA+FIG N R GS +V G H ++ + +G Sbjct: 243 YPDNQFVANFIGDNN-----RFKGSVESVQGEHCTVRLTDGAALTGLNVHRAHAGQSITA 297 Query: 289 FCRPEAVQLFSDESCA-NRFLARVVDRIYLGDRYRL 323 RPE ++L + S N A + IY GD R+ Sbjct: 298 CVRPERIRLAAGGSAEPNSIRASIAGLIYFGDHVRV 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 958,980 Number of extensions: 36323 Number of successful extensions: 526 Number of sequences better than 1.0e-08: 133 Number of HSP's gapped: 321 Number of HSP's successfully gapped: 149 Length of query: 369 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 282 Effective length of database: 1,207,925 Effective search space: 340634850 Effective search space used: 340634850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)