BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0041.p (374 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 631 0.0 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 196 2e-51 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 160 1e-40 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 156 3e-39 gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC fami... 150 1e-37 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 631 bits (1628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 329/379 (86%), Positives = 345/379 (91%), Gaps = 10/379 (2%) Query: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR 60 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR Sbjct: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR 60 Query: 61 IGFKGTEDLGGGLKAMFQLENGFNSDTGAMSNSGVLFNRQSWVGLQGGFGKVRVGRQNTP 120 IGFKGTEDLGGGLKAMFQLENGF+SDTGAM G LF RQSWVGLQGGFGKVRVGRQNTP Sbjct: 61 IGFKGTEDLGGGLKAMFQLENGFSSDTGAMQQGGSLFGRQSWVGLQGGFGKVRVGRQNTP 120 Query: 121 MFDLLADHFDPLTVGNYASNSWLPVAATLARRSNMLRYDGDFGPVSASVSWGFGETAGSV 180 +FDLLADHFDPLTVGNYASNSWLP AA+L R NMLRYDGDFGPV+AS SW FGE AGSV Sbjct: 121 LFDLLADHFDPLTVGNYASNSWLPAAASLVRSPNMLRYDGDFGPVAASASWAFGEKAGSV 180 Query: 181 RTGSQFGSSLRYTAGAFAVGGGYQQTVSGTDSSLKHTSWTLSASYDFGVAKVFGGWINIK 240 RTGSQF S+LRYTAG A+GGGYQQTVS T+SSLKHT+W LSASYDFGVAKVFGGW+NIK Sbjct: 181 RTGSQFSSALRYTAGPLALGGGYQQTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIK 240 Query: 241 DNSGITGSVMAADSDIGTQWTSAGTLAAAANN-----QRKDNGFFLGSSYKATNALTLTG 295 D+SG+TGSVMAAD D+ WT+AG AANN +RKDNGFFLGSSYKATNALTLTG Sbjct: 241 DDSGLTGSVMAADGDVSNGWTTAG----AANNGSTGVKRKDNGFFLGSSYKATNALTLTG 296 Query: 296 AFYYDRSKNVAYAAPVGNVGDGKRYALVGWADYALSKRTQLYTTIDYNKAKDAAVYELTN 355 AFYYDRSKNV+Y + GNVGDGKRY LVGWADYALSKRTQLYTTIDYNKAKDAAVYELTN Sbjct: 297 AFYYDRSKNVSYVS-TGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDAAVYELTN 355 Query: 356 SANGKNSLTTVGVGIRHIF 374 SANGKNSLTTVGVGIRHIF Sbjct: 356 SANGKNSLTTVGVGIRHIF 374 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 196 bits (498), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 147/372 (39%), Positives = 201/372 (54%), Gaps = 48/372 (12%) Query: 17 GAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNV-RMDNGAIANSRIGFKGTEDLGGGLKA 75 G+ AQS+VTIYG+VDT + YL+NA G +V RM +GA SRIGF+GTEDLGGGLKA Sbjct: 17 GSVQAQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKA 76 Query: 76 MFQLENGFNSDTGAMS-NSGVLFNRQSWVGLQGGFGKVRVGRQNTPMFDLLADHFDPLTV 134 +FQLENGF D G+ ++ LFNRQS VGL+GGFG+V GR + +D L FDP+ Sbjct: 77 LFQLENGFKLDNGSFDGDANQLFNRQSTVGLEGGFGRVVAGRSYSTTYDFLGP-FDPM-- 133 Query: 135 GNYASNSWLPVAATLARR--------SNMLRYDGDFGPVSASVSWGFGETAGSVRTGSQF 186 G A+ SW A R SN+++Y G+F V +GFGE AG R G+++ Sbjct: 134 GYSANYSWATTAGATGNRKDGMPTGVSNLVKYQGEFSGVKIGAMYGFGEVAGETRNGAKY 193 Query: 187 GSSLRYTAGAFAVGGGYQQTVSGTDSSLKH---TSWTLSASYDFGVAKVFGGWINIKDNS 243 G L + G F+V Y + S T ++ + TS L+A Y A F Sbjct: 194 GVGLGWGTGPFSVVATYDRNNSATATNTPYDRATSAHLAAGYQPSEAWKF---------- 243 Query: 244 GITGSVMAADSDIGTQWTSAGTLAAAANNQRKDNGFFLGSSYKATNALTLTGAFYYDRSK 303 ++G ++ +LA A + R D F+ G++YK T A+TL GA YY K Sbjct: 244 -----------NLGYRYYKK-SLATGAADLRSDF-FWGGAAYKFTPAMTLIGAIYYQNIK 290 Query: 304 NVAYAAPVGNVGDGKRYALVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSANGK-NS 362 NVA N D Y+L YA+SKRT LY + Y KAK+ + L+ G +S Sbjct: 291 NVA------NDADPIMYSLR--LKYAMSKRTDLYLSTAYAKAKNGQLVGLSRDDAGYGSS 342 Query: 363 LTTVGVGIRHIF 374 + +G++H F Sbjct: 343 QFGMTLGMQHRF 354 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 160 bits (405), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 138/428 (32%), Positives = 199/428 (46%), Gaps = 77/428 (17%) Query: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGN---SNVRMDNGAIA 57 MKKS L L G +G A S++TIYG +D S+ Y + G S +++G + Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDGKNTGSLTAVESGLVK 60 Query: 58 NSRIGFKGTEDLGGGLKAMFQLENGFNSDTGAMSNSGVLFNRQSWVGLQGGFGKVRVGRQ 117 SR+G KG+EDLGGGLKA+F LENGFN DTG +++ RQ+ VGL G FG V VGRQ Sbjct: 61 GSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGLSGNFGTVLVGRQ 120 Query: 118 NTPMFDLLADHFDPLTVGNYASNSWLPVAATL----------ARRSNMLRYDG-DFGPVS 166 N P+ D +G AS V T+ R +N +RY+ +F +S Sbjct: 121 NDPLND----------IGGIASAGGFGVPGTVHSKGLDRTDGQRLNNSIRYNSPNFNGLS 170 Query: 167 ASVSWGFGETAGSVRTGSQFGSSLRYTAGAFAVGGGYQQTVSG----------------- 209 S +GFGE G G G +Y G ++G GY Q+ G Sbjct: 171 FSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACNG 230 Query: 210 -------TDSSLKHTSWTLSASYDFGVAKVFGGWINI------------KDNSGITGSVM 250 D+ LK +WTL+A+Y FG AKV+G + ++ KDN+ G++ Sbjct: 231 AGEKDHAGDTCLK--TWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNN--FGAMY 286 Query: 251 AADSDIGTQWTSAGTLAAAANNQRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVAYAAP 310 A + G + A + N + F +G SY+ +LTL A + R+K V A+ Sbjct: 287 DAKTGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFVGAAS- 345 Query: 311 VGNVGDGKRYALVGWADYALSKRTQLYTTIDYNKAKDAAVYELTNSANGK----NSLTTV 366 G+ L A Y LSKRT Y + ++ D +Y + S + + S T + Sbjct: 346 ------GRLLQLNLGAKYDLSKRTSAYALVRNLRSSD--MYNVGLSKDQEPGIDRSQTAI 397 Query: 367 GVGIRHIF 374 G+ H F Sbjct: 398 STGLIHTF 405 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 156 bits (394), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 147/405 (36%), Positives = 207/405 (51%), Gaps = 46/405 (11%) Query: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSN-ANAQGN--SNVRMDNGAIA 57 MKKS LL LA GAA AQS+VTIYGIVDT + Y S A G S +++G I Sbjct: 1 MKKS-LLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQ 59 Query: 58 NSRIGFKGTEDLGGGLKAMFQLENGFNSDTGAMSN---SGVLFNRQSWVGLQGGFGKVRV 114 SRIGFKGTEDLGGGL A+F LE GF++DTGA+ + S LF R+S VGL G FG V + Sbjct: 60 GSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLL 119 Query: 115 GRQNTPMFDLLADHFDPLTVGNY---ASNSWLPVAATLARRSNMLRY-DGDFGPVSASVS 170 GRQ T D ++ + G + A ++ + T R +N + Y + + ++ Sbjct: 120 GRQ-TDFADTISSYTSVQDFGGWTGAAGHNLDRLEGT--RTNNSISYTTANLNGFTGNLM 176 Query: 171 WGFGETAGSVRTGSQFGSSLRYTAGAFAVGGGYQQTVSGT---DSSLKHTSWT------- 220 +GFGETAG G FG +Y G +G Y Q+ +G+ D+SL + T Sbjct: 177 YGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATGTNQFTNGT 236 Query: 221 -----------LSASYDFGVAKVFGGWINIKDNSGITGSVMAADSDIGTQWTSAGTLAAA 269 ++ASY FG A+V+G W +K + ++ AA +G+ T + AA Sbjct: 237 NNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAA--GVGSLATISNRTLAA 294 Query: 270 ANNQRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVAYAAPVGNVGDGKRYALVGWADYA 329 +N K + + +G++Y + +L L A + R A G G K + DY Sbjct: 295 SN---KADIYEIGTAYSLSPSLKLLAAVDHTR------AQFDGVSGKAKLTQVSLGTDYW 345 Query: 330 LSKRTQLYTTIDYNKAKDAAVYELTNSANGKNSLTTVGVGIRHIF 374 LSKRT LY + Y+KA D + +S+ + V VGIRH F Sbjct: 346 LSKRTDLYAFLAYSKATDLNNPGVADSSGTDGNQGAVAVGIRHKF 390 >gi|300310663|ref|YP_003774755.1| outer membrane porin, OmpC family protein [Herbaspirillum seropedicae SmR1] Length = 346 Score = 150 bits (380), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 136/391 (34%), Positives = 201/391 (51%), Gaps = 62/391 (15%) Query: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR 60 MKK+ + +A+ L +GGA AQSNVT+YG++DT + Y +NA +G S +D+G SR Sbjct: 1 MKKAWAVGVASLLCAGGA-LAQSNVTLYGVIDTYMGY-TNATGKG-SVASLDSGGYQASR 57 Query: 61 IGFKGTEDLGGGLKAMFQLENGFNSDTGAMSNSGVLFNRQSWVGLQGGFGKVRVGRQNTP 120 +GFKG+EDLGGGL+A FQ+ENGF SD+GAM +S LFNRQSWVGL G++R+GRQN+P Sbjct: 58 VGFKGSEDLGGGLRANFQMENGFASDSGAMHDSTRLFNRQSWVGLGNELGELRLGRQNSP 117 Query: 121 MFDLLA--DHFDPLTVGNYASNSWLPVAATLARRSNMLRYDGDFGPV----SASVSWGFG 174 F ++A D F T ++ +N V+A R N++ Y PV +G G Sbjct: 118 GFLMIARLDAFAGATYASFLNN----VSAYTPRYDNVIGY---LSPVMRGFRVQAYYGLG 170 Query: 175 ETAGSVRTGSQFGSSLRYTAGAFAVGGGYQQTVSGTDSS----LKHTSWTLSASYDFGVA 230 E +T + G S AG + G Y S +S + S +YD+G Sbjct: 171 E-----QTAPRNGLSTAMLAGEYESGPLYLGVSSSAQNSANDNITIRSTFAGGAYDYGSG 225 Query: 231 KVFGGWINIKDNSGITGSVMAADSDIGTQWTSAGTLAAAANNQRKDNGFFLGSSYKATNA 290 KVF G+ +N G AA ++ + SA +L+A +Y+ T Sbjct: 226 KVFLGYYR-GNNLG-----AAASANTPGSYYSAYSLSA---------------NYRLTGQ 264 Query: 291 LTLTGAFYYDRSKNVAYAAPVGNVGDGKRYALVGWADYALSKRTQLYTTIDYNKAKDAAV 350 TL + + + G + ++++L+G Y LS+RT LY T + + A Sbjct: 265 ATLGAGYGWAKDS-------TGAGRNARQFSLIG--TYDLSRRTMLYATYAHLANNNGAS 315 Query: 351 YELTNSAN-GKNSLTT------VGVGIRHIF 374 + L +A KN+ + V VGIRH+F Sbjct: 316 FSLAGAAPITKNAPVSGGTVNGVQVGIRHLF 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,168,901 Number of extensions: 52759 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 14 Length of query: 374 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 287 Effective length of database: 1,207,925 Effective search space: 346674475 Effective search space used: 346674475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)