BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0030.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 865 0.0 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 503 e-143 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 424 e-120 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 380 e-106 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 362 e-101 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 865 bits (2234), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/435 (99%), Positives = 432/435 (99%) Query: 1 MSYQAGAAANGARASFEDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 MSYQAGAAA+GARASFEDATY KVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS Sbjct: 1 MSYQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 Query: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI Sbjct: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 Query: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG Sbjct: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 Query: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQKEH 240 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLE NISHEESQKEH Sbjct: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEH 240 Query: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA Sbjct: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 Query: 301 AAMVLIGRSADARHERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLP 360 AAMVLIGRSADAR ERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLP Sbjct: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLP 360 Query: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVI 420 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVI Sbjct: 361 LFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVI 420 Query: 421 GGLLTLTLPAKLVNR 435 GGLLTLTLPAKLVNR Sbjct: 421 GGLLTLTLPAKLVNR 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 503 bits (1294), Expect = e-143, Method: Compositional matrix adjust. Identities = 245/420 (58%), Positives = 321/420 (76%), Gaps = 1/420 (0%) Query: 17 EDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 +D Y KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FS+TV+GLGAG+FF+GYF Sbjct: 18 KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYF 77 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 +FEVPSN+++HKVGAR+WIARIMITWGI+S A +V FYI+RFLLG+AEAGF+PG+ Sbjct: 78 LFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGI 137 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 ILYLTYW+P+ RR K+ A+FM+GI ++G++G PLSGWIM A G GWQWMF++EAI Sbjct: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 Query: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQKE-HLSLGAMFANGRVWLG 255 P++++GV +LY+ + ++ A WL++EEK L+ +I + KE GA+ + RVWL Sbjct: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLM 257 Query: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARHE 315 LIYF FVMG YG++ W+PT++KATGV L +GLL+AIP+ A AM LIGRSAD E Sbjct: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 Query: 316 RRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAA 375 RRWH+ IPA +G +G + + + NT +++ASL+LA+ G+LT PLFWSLPTAFLSG AA Sbjct: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 Query: 376 AAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAKLVNR 435 A GIA INS+GNLAGFVSP+L+G+LKD T + GMY+LA LV+G + TL +VN+ Sbjct: 378 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPGMVNK 437 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 424 bits (1090), Expect = e-120, Method: Compositional matrix adjust. Identities = 221/415 (53%), Positives = 292/415 (70%), Gaps = 1/415 (0%) Query: 18 DATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FYI+RFLLG+ EAGFFPGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 LYLTYW+PA RR ++ LFMT A++G +GGP++G IM M GV WQW+FILE IP Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 198 SLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQK-EHLSLGAMFANGRVWLGA 256 S++ G V+ ++ +R +A WLSE EK + ++ E S +H S N RVW+ A Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICA 261 Query: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARHER 316 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 R H AI I CIGL+ + N TLA+ SLTLA++GIL P+FWS+P AFL+GTAAA Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAA 381 Query: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAK 431 GIALINS+GNLAGFV+P+++G LK +T S ++G+Y +AA ++ L + K Sbjct: 382 GGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 380 bits (975), Expect = e-106, Method: Compositional matrix adjust. Identities = 201/426 (47%), Positives = 284/426 (66%), Gaps = 10/426 (2%) Query: 8 AANGARASFEDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLG 67 A+ G+ F D Y KVTW++LP L L YV +YLDRVNVGFAKL ML DL S+ YGLG Sbjct: 4 ASQGSAVQF-DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLG 62 Query: 68 AGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAY---------VTTPEMF 118 AGIFFLGYF+FEVPSN+ILH+VGA++WIARIMITWG+IS MA+ V+ +F Sbjct: 63 AGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVF 122 Query: 119 YIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAM 178 Y +RFLLG +EAGFFPGVILY+TYWYPAA ++ A+ + G S ++GGP+SG +M M Sbjct: 123 YTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFM 182 Query: 179 PGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQK 238 GWQWMF++EA+P+ +LG++++ + + I A WL+ EEK L ++ E +K Sbjct: 183 GSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK 242 Query: 239 EHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAF 298 ++ L +++A +W AL+Y ++G YG++FW PTII+ GV L++G+++AIPY Sbjct: 243 VNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYIL 302 Query: 299 AAAAMVLIGRSADARHERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTT 358 A AMV I R A+ + R + + + + L+L T + HN + +A + +A G LT+ Sbjct: 303 GATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTS 362 Query: 359 LPLFWSLPTAFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASL 418 + LFW P A+ +G AAAGIA+INS+GNL GFV P+L+G L A S G+ +L +L Sbjct: 363 IALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGAL 422 Query: 419 VIGGLL 424 + G+L Sbjct: 423 FLAGVL 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 362 bits (928), Expect = e-101, Method: Compositional matrix adjust. Identities = 192/415 (46%), Positives = 271/415 (65%), Gaps = 6/415 (1%) Query: 17 EDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 EDAT KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FF+GYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 LY T W+P A RGK TA F+ GI ++ +IG PLSG +M + G+WG GWQ + +LEA+ Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 198 Query: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEES---QKEHLSLGAMFANGRVW 253 P+++LGVM + + DR A WLS +EK+ LE +S E++ + L F N RV+ Sbjct: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 Query: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 A FC ++G + WLP II+ G+ VGL+ AIPY A AM L R A+ Sbjct: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRS 317 Query: 314 HERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGT 373 R + VA + + L +S + H L M ++T+A IL+ FW++P+ FL+G Sbjct: 318 ERRLFFVAGAIVLAALSLGISA-FLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 376 Query: 374 AAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL 428 AAA G+ALI S+GNL GFV P ++G++++ATQ + +A +L++G ++TL L Sbjct: 377 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLAL 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,436,612 Number of extensions: 64470 Number of successful extensions: 427 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 21 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)