BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0029.p (367 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 680 0.0 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 421 e-119 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 367 e-102 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 343 1e-95 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 310 1e-85 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/361 (97%), Positives = 355/361 (98%), Gaps = 1/361 (0%) Query: 1 MSYQAGAAANGARASFEDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 MSYQAGAAA+GARASFEDATY KVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS Sbjct: 1 MSYQAGAAADGARASFEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFS 60 Query: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI Sbjct: 61 DTVYGLGAGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYI 120 Query: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG Sbjct: 121 MRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPG 180 Query: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQKEH 240 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLE NISHEESQKEH Sbjct: 181 VWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEH 240 Query: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA Sbjct: 241 LSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAA 300 Query: 301 AAMVLIGRSADARHERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLAIGGLLTLTL 360 AAMVLIGRSADAR ERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLA G+LT TL Sbjct: 301 AAMVLIGRSADARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILT-TL 359 Query: 361 P 361 P Sbjct: 360 P 360 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 421 bits (1083), Expect = e-119, Method: Compositional matrix adjust. Identities = 196/342 (57%), Positives = 261/342 (76%), Gaps = 1/342 (0%) Query: 17 EDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 +D Y KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FS+TV+GLGAG+FF+GYF Sbjct: 18 KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYF 77 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 +FEVPSN+++HKVGAR+WIARIMITWGI+S A +V FYI+RFLLG+AEAGF+PG+ Sbjct: 78 LFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGI 137 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 ILYLTYW+P+ RR K+ A+FM+GI ++G++G PLSGWIM A G GWQWMF++EAI Sbjct: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 Query: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQKEHL-SLGAMFANGRVWLG 255 P++++GV +LY+ + ++ A WL++EEK L+ +I + KE GA+ + RVWL Sbjct: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLM 257 Query: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARHE 315 LIYF FVMG YG++ W+PT++KATGV L +GLL+AIP+ A AM LIGRSAD E Sbjct: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 Query: 316 RRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLAIGGLLT 357 RRWH+ IPA +G +G + + + NT +++ASL+LA G+LT Sbjct: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLT 359 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 181/340 (53%), Positives = 235/340 (69%), Gaps = 1/340 (0%) Query: 18 DATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGVI 137 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FYI+RFLLG+ EAGFFPGV+ Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 138 LYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAIP 197 LYLTYW+PA RR ++ LFMT A++G +GGP++G IM M GV WQW+FILE IP Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 198 SLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQK-EHLSLGAMFANGRVWLGA 256 S++ G V+ ++ +R +A WLSE EK + ++ E S +H S N RVW+ A Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICA 261 Query: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARHER 316 +YFC V G ++FW P+IIK GV L +GL++AIP+ AM+ G +D ER Sbjct: 262 AVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGER 321 Query: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLAIGGLL 356 R H AI I CIGL+ + N TLA+ SLTLA G+L Sbjct: 322 RLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGIL 361 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 343 bits (880), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 170/359 (47%), Positives = 241/359 (67%), Gaps = 10/359 (2%) Query: 8 AANGARASFEDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLG 67 A+ G+ F D Y KVTW++LP L L YV +YLDRVNVGFAKL ML DL S+ YGLG Sbjct: 4 ASQGSAVQF-DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLG 62 Query: 68 AGIFFLGYFVFEVPSNMILHKVGARLWIARIMITWGIISGAMAY---------VTTPEMF 118 AGIFFLGYF+FEVPSN+ILH+VGA++WIARIMITWG+IS MA+ V+ +F Sbjct: 63 AGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVF 122 Query: 119 YIMRFLLGVAEAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAM 178 Y +RFLLG +EAGFFPGVILY+TYWYPAA ++ A+ + G S ++GGP+SG +M M Sbjct: 123 YTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFM 182 Query: 179 PGVWGHTGWQWMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQK 238 GWQWMF++EA+P+ +LG++++ + + I A WL+ EEK L ++ E +K Sbjct: 183 GSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK 242 Query: 239 EHLSLGAMFANGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAF 298 ++ L +++A +W AL+Y ++G YG++FW PTII+ GV L++G+++AIPY Sbjct: 243 VNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYIL 302 Query: 299 AAAAMVLIGRSADARHERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLAIGGLLT 357 A AMV I R A+ + R + + + + L+L T + HN + +A + +A+GG LT Sbjct: 303 GATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLT 361 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 310 bits (794), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 150/312 (48%), Positives = 204/312 (65%), Gaps = 6/312 (1%) Query: 17 EDATYGKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 EDA Y KV R++P LF+CYV S+LDR+N+GFA+LQM QDL FSD +YGLGA IF++GY Sbjct: 21 EDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYV 80 Query: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 +FEVPSNM L + GAR +RIM+ WG+ S M VT FY +RFLLGV EAGFFPG+ Sbjct: 81 LFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGI 140 Query: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 +LYL+YW+PA RR + A+F G+A++GV+GG +SGWIM M GV G GWQWMF +E Sbjct: 141 VLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 200 Query: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLETNISHEESQKEHLS------LGAMFANG 250 P+++LG++ I + D A WLS EE+ L+ E + H+S L ++ Sbjct: 201 PAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALSHR 260 Query: 251 RVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSA 310 +L A IYF G ++FW+P +I+ G+ D + V L + IP A A ++LI R + Sbjct: 261 TTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRS 320 Query: 311 DARHERRWHVAI 322 D R+ER H A+ Sbjct: 321 DRRNERHGHFAV 332 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,153,593 Number of extensions: 50653 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 288 Number of HSP's successfully gapped: 20 Length of query: 367 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 280 Effective length of database: 1,207,925 Effective search space: 338219000 Effective search space used: 338219000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)