BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0028.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 511 e-146 gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 144 7e-36 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 142 3e-35 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 139 3e-34 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 137 6e-34 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 511 bits (1315), Expect = e-146, Method: Compositional matrix adjust. Identities = 252/252 (100%), Positives = 252/252 (100%) Query: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 Query: 241 GVCIEVDGGRCV 252 GVCIEVDGGRCV Sbjct: 241 GVCIEVDGGRCV 252 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 144 bits (363), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 93/264 (35%), Positives = 142/264 (53%), Gaps = 19/264 (7%) Query: 3 LQDKVAIVTGGGS---GFGEGIAKA--YAREGAAIVVADIGEAGGLRVVEEIKAAGGRAV 57 +Q K IVTG GS G+G G A A YAREG ++ D L+ + I++ GG Sbjct: 5 MQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEGGVCE 64 Query: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKS 117 ADVS+ ++ + A++HFG++D++ NN G P +E E+ ++R+ AVN S Sbjct: 65 VLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGP-VETSEESWNRLVAVNQTS 123 Query: 118 IFLSAKTFVPYFRQVGGGAFINIASTAGIR----PRPGLTWYNGSKGAVITTSKSMAAEL 173 +FL+ K +P + GA +NI+S A +R P G Y +K A++ +K++A + Sbjct: 124 LFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLG---YTATKAAILAFTKNVAMQY 180 Query: 174 GPDKIRVNCVNPVISATGLLSEFMGVP---DTPENRKKFVATIPMGRFSTPEDIANACLY 230 P IR NCV P + T ++ E + D + R K A PMG D+A+A L+ Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 231 LGSDEAEFVTGVCIEVDGG---RC 251 L SD A ++TG+ + VDGG RC Sbjct: 241 LASDHARYITGLDMIVDGGLSLRC 264 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 142 bits (358), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 86/249 (34%), Positives = 136/249 (54%), Gaps = 7/249 (2%) Query: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 R + KV IVTG SG GE A+ ++ EGA +++AD A +V + + R + Sbjct: 10 QRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPE--RTAARE 67 Query: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 DVS + QL+ A+E FG+LD++V++AG + EV +++ RV A NV +F Sbjct: 68 TDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGN-VTEVSPEDWHRVQATNVNGVFY 126 Query: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 A+ +P+ + G +N+AS +G+ L+ YN SKGAV +++MA + G +R+ Sbjct: 127 GAREALPHLEKTRG-CIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRI 185 Query: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEAEFVT 240 N V P ++ T + ++ D P KF I +GR + P +IA +L S +A FV Sbjct: 186 NAVCPSLTHTAMTADMA---DDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVN 242 Query: 241 GVCIEVDGG 249 GV + VDGG Sbjct: 243 GVNLPVDGG 251 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 139 bits (349), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 88/253 (34%), Positives = 139/253 (54%), Gaps = 16/253 (6%) Query: 2 RLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVV----ADIGEAGGLRVVEEIKAAGGRAV 57 R +DKV +VTGG G G AK +A EGA + V AD ++ + ++ G AV Sbjct: 6 RFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRAD-------KLEQAVREIGNGAV 58 Query: 58 FAKADVSKRADMDQLLATALEHFGKLDIVVNNAGT-THRNRPMLEVEEDEFDRVYAVNVK 116 D +K D+D+L A G+LD++ NAGT + +P+ + E++FD ++ +NV+ Sbjct: 59 GVPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVR 118 Query: 117 SIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPD 176 + S + +P Q GGA + S AG++ PG + YN SK AV + ++S A+L Sbjct: 119 GLLFSVQKALPLMSQ--GGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKER 176 Query: 177 KIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGSDEA 236 IRVN V P + T L+ +++ E+ K V +P+GR P++I A L+L S E+ Sbjct: 177 GIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQV--VPLGRLGEPDEIGRAVLFLASAES 234 Query: 237 EFVTGVCIEVDGG 249 ++ GV + VDGG Sbjct: 235 SYIAGVELFVDGG 247 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 137 bits (346), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 84/259 (32%), Positives = 144/259 (55%), Gaps = 18/259 (6%) Query: 1 MRLQDKVAIVTGGG--SGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAA---GGR 55 M L+DKVAI+TGG +G G A+ A +GA IV+ D+ A E AA G + Sbjct: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERA------EPAGAAARLGAQ 54 Query: 56 AVFAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNV 115 + ADV+ +A + A L+ FG++DI+VNNAG T + +LE+ ++DR+ V++ Sbjct: 55 HLGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAK-LLEITGKDYDRILDVSL 113 Query: 116 KSIFLSAKTFVPYFRQVGGGAFINIASTAGIRPRP--GLTWYNGSKGAVITTSKSMAAEL 173 + ++ +P + G+ + I+S + R G Y+ +K V+ +++MA E Sbjct: 114 RGTLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREF 173 Query: 174 GPDKIRVNCVNPVISATGLLSEFMGVPDTPENRKKFVATIPMGRFSTPEDIANACLYLGS 233 GP IRVNC+ P + +T + + + + E + + TIP+GR P+D+ C++L S Sbjct: 174 GPQGIRVNCITPGLISTDINKDIL----SAEKKAEIAQTIPLGRLGGPDDVGGVCVFLAS 229 Query: 234 DEAEFVTGVCIEVDGGRCV 252 D +++ TGV ++V+GG + Sbjct: 230 DLSQYCTGVTLDVNGGMLI 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 809,257 Number of extensions: 34069 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 52 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)