BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0027.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 967 0.0 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 305 6e-84 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 278 6e-76 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 274 1e-74 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 271 7e-74 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 967 bits (2499), Expect = 0.0, Method: Compositional matrix adjust. Identities = 472/477 (98%), Positives = 474/477 (99%) Query: 1 MKQHFIANRWVAPASGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 MKQHFIANRWVAP SGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSL 180 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLS+ Sbjct: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI 180 Query: 181 LRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHH 240 LRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHH Sbjct: 181 LRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHH 240 Query: 241 TPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGVYEQVLERL 300 TPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRG YEQVLERL Sbjct: 241 TPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERL 300 Query: 301 SGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPAGGFY 360 SGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVP GGFY Sbjct: 301 SGLFSQLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFY 360 Query: 361 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAVALANGTDYGLVASVWTRDGGRQM 420 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHA+ALANGTDYGLVASVWTRDGGRQM Sbjct: 361 QAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQM 420 Query: 421 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG 477 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG Sbjct: 421 RLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAIRHG 477 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 305 bits (780), Expect = 6e-84, Method: Compositional matrix adjust. Identities = 192/480 (40%), Positives = 274/480 (57%), Gaps = 13/480 (2%) Query: 3 QHFIANRWVAPASGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEG-AWGRLSA 61 QHFIA + G+ + +++P + + + + A+G A D+ +AV AA+ EG AW +LS Sbjct: 6 QHFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSG 65 Query: 62 AERSRLLMKLAYKLQDHQEELAQLEGRDCGK-PMKQARADAAAIVRYFEFYAGAADKLHG 120 +R +LL KLA ++ + LA ++ R G+ PM+ D + + AG A+++ G Sbjct: 66 LQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEG 125 Query: 121 DTIPYQAGYT-----VLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 TIP AGY T+REP GV G I+PWN P+ I V LAAG T V+KPAE+ Sbjct: 126 RTIP-TAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEE 184 Query: 176 ACLSLLRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRA 235 S L +A LA E GFP+G +N+VTGYG+ G L +HPDV ISFTGSP G+ + R Sbjct: 185 TPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRI 244 Query: 236 AAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGVYEQ 295 A E VTLELGGKSPQIVF+DA E L + N GQ C+AGSR+LVQR + ++ Sbjct: 245 AGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQR 304 Query: 296 VLERLSGLFSQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGV 354 + L+ + + VG SQ + GP+ +K Q ERV ++ +G L A G Sbjct: 305 FAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG---VSR 361 Query: 355 PAGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAVALANGTDYGLVASVWTR 414 P G++ P + D +A+EE+FGPV + F+ E A+ALAN + YGL A+VWT Sbjct: 362 PDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTT 421 Query: 415 DGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 + R R+A ++ G V +N + + LP+GG+K SG GRE G +T KT+ + Sbjct: 422 NLARAHRVAAGVKVGAVGVNCW-SPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVTV 480 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 278 bits (711), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 189/454 (41%), Positives = 255/454 (56%), Gaps = 26/454 (5%) Query: 4 HFIANRWVAPASGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAE 63 HFIA R+ P + + +DP+ G+ +++G+A D+D AV+AA+ A G W L Sbjct: 35 HFIAGRFTQPKA--DLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPG-WQALGGHG 91 Query: 64 RSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQAR-ADAAAIVRYFEFYAGAADKLHGDT 122 R+R L LA +Q H LA LE D GKP++++R D + R+F YAG A +L Sbjct: 92 RARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWA-QLQESE 150 Query: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLR 182 P P GV G IVPWN+P+ + + ALA GNT V+KPAE+ L+ L Sbjct: 151 FPDHV---------PVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTALL 201 Query: 183 VAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTP 242 AELA + G P G LNIVTG G G +V HP + I+FTGS VG+L+ A Sbjct: 202 FAELAQQAGLPAGVLNIVTGDG-ATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGKS 260 Query: 243 VTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGVYEQVLERLSG 302 +TLELGGKSP IVFEDAD++A + VV+AI N GQ C AGSRLLVQ G+++ + RL Sbjct: 261 LTLELGGKSPFIVFEDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKT 320 Query: 303 LFSQLQVGSSQMDL-DCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPAGGFYQ 361 +L+VG+ D G LI Q ERV + R+G A ++ P GG + Sbjct: 321 RMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREG---AQCHQVVLDTPPGGCFY 377 Query: 362 APALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAVALANGTDYGLVASVWTRDGGRQMR 421 P LL V P +A EE+FGPVL AM F AV LAN + YGL AS+W+ G + Sbjct: 378 PPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALG 437 Query: 422 LARAIRSGQVFINN---YGAGGGVELPFGGVKAS 452 +A +++G V+IN+ + A G FGGV+ S Sbjct: 438 VAPQLKAGVVWINSTNQFDAAVG----FGGVRES 467 Score = 65.9 bits (159), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 90/220 (40%), Gaps = 3/220 (1%) Query: 5 FIANRWVAPASGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAER 64 +I + V P GE + G+ + + GN DI AV AA G W S R Sbjct: 521 YIGGKQVRP-DGEVSMACHSTQGERLEDVGLGNRKDIRNAVAAA--VKAGGWTSASPHRR 577 Query: 65 SRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHGDTIP 124 ++ L +A L EE A+ G +ARA+ A ++ Y ADK G Sbjct: 578 AQGLYYIAENLSARTEEFARRIASTTGVTADEARAEVDASIKRLFTYGAWADKFEGAVHQ 637 Query: 125 YQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLRVA 184 L + E GV G + P P+ V +A GN VV P+E A L + Sbjct: 638 PPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLVATDLY 697 Query: 185 ELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTG 224 ++ P G +NIVTG E +L +H +V + G Sbjct: 698 QVLDTSDLPAGVINIVTGKSAELLPVLAEHDEVEALWVRG 737 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 274 bits (700), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 160/420 (38%), Positives = 231/420 (55%), Gaps = 5/420 (1%) Query: 56 WGRLSAAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAA 115 W LS A R R L + ++ E LA+LE GKP++ RA+ + FE+YAG A Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWA 134 Query: 116 DKLHGDTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAED 175 DK +G+ IP + + T REP+G + PWN P G +G ALA GN ++KP+E Sbjct: 135 DKFYGEVIPVPSSHLNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSEL 194 Query: 176 ACLSLLRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRA 235 S L +A L + G P G +N++ G G + V + F GSP G L+ +A Sbjct: 195 TPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKA 254 Query: 236 AAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGVYEQ 295 AA P LELGGKS I+FEDAD+ AI AGQ+C AGSRLLVQR VY++ Sbjct: 255 AAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDR 314 Query: 296 VLERLSGLFSQLQVGSSQMD-LDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGV 354 +E ++ ++++G+ D + GP+ + Q E + ++ G LAA G G Sbjct: 315 FVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAA-GHTRYGE 373 Query: 355 PAGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAVALANGTDYGLVASVWTR 414 G++ P LL +A+ E+FGPV A+PFEDE A+A+AN +++GL +VWTR Sbjct: 374 E--GYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTR 431 Query: 415 DGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTIAI 474 D R R+A ++ +G ++N+Y V PFGG SG+GR G EALY +T K++ + Sbjct: 432 DVARAHRVAASVNAGTFWVNSYKT-INVASPFGGFNRSGYGRSSGMEALYDYTQTKSVWV 490 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 271 bits (694), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 167/469 (35%), Positives = 260/469 (55%), Gaps = 23/469 (4%) Query: 4 HFIANRWVAPASGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAE 63 +FI ++V P GE + P G+ F +AR +A D++ A+ AA A + +WG+ S E Sbjct: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA-KKSWGKTSPTE 79 Query: 64 RSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGDT 122 R+ +L+K+A +++ + E LA E D GKP+++ A D + +F ++A A G Sbjct: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 Query: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSLLR 182 P EP GV G I+PWN+P+ + + ALAAGN V+KPAE S++ Sbjct: 140 CPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMV 199 Query: 183 VAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTP 242 + EL A++ P G +NIV G+G EAG L + + I+FTG G+L+++ A+++ P Sbjct: 200 LIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIP 258 Query: 243 VTLELGGKSPQIVFEDA-------DMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGVYEQ 295 VTLELGGKSP I F D +A+ + A+ N G+ C+ SR+LVQ +YE+ Sbjct: 259 VTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFAL--NQGEVCTCPSRVLVQESIYER 316 Query: 296 VLERLSGLFSQLQVGSSQMDLDCGPLI----RKTQQERVAGFLSDAQRDGIALAAQG--K 349 +ER + ++ G+ LD +I + Q E++ ++ +++G + A G + Sbjct: 317 FIERALKRVAAIKQGNP---LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGRE 373 Query: 350 IAAGVPAGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAVALANGTDYGLVA 409 G A G+Y P + + + R+ QEE+FGPV++ F+DE A+A+AN T YGL A Sbjct: 374 ELGGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGA 432 Query: 410 SVWTRDGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREK 458 +WTRDG R R+ R I++G+V+ N Y FGG K SG GRE Sbjct: 433 GLWTRDGTRAFRMGREIQAGRVWTNCYHLYPA-HAAFGGYKQSGIGREN 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,695,222 Number of extensions: 79602 Number of successful extensions: 344 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 267 Number of HSP's successfully gapped: 22 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)