BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0022.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311572|ref|YP_003775664.1| transmembrane protein [Herbaspi... 798 0.0 gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767... 115 8e-27 gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodies... 108 1e-24 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 107 1e-24 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 107 2e-24 >gi|300311572|ref|YP_003775664.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 798 bits (2060), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/395 (99%), Positives = 394/395 (99%) Query: 1 MNAATASIALALVQFCGGLIMAGLFMNMPKEHCTRLWALSGLCGAAGICLMLLGYNHMGG 60 MNAATASIALALVQFCGGLIMAGLFMNMPKEHCTRLWALSGLCGAAGICLML+GYNHMG Sbjct: 1 MNAATASIALALVQFCGGLIMAGLFMNMPKEHCTRLWALSGLCGAAGICLMLVGYNHMGD 60 Query: 61 VWNPLALLAGNTGLFSCCLLAWAGLRSFYQRRTGWWPWLLVGFYAFLFSLLLLYQVSFTQ 120 VWNPLALLAGNTGLFSCCLLAWAGLRSFYQRRTGWWPWLLVGFYAFLFSLLLLYQVSFTQ Sbjct: 61 VWNPLALLAGNTGLFSCCLLAWAGLRSFYQRRTGWWPWLLVGFYAFLFSLLLLYQVSFTQ 120 Query: 121 RSYLALVALQLTFWLALGEFARGMSGPHAQRYARWSFGRCIGIVSLLIFISTHVARFILS 180 RSYLALVALQLTFWLALGEFARGMSGPHAQRYARWSFGRCIGIVSLLIFISTHVARF+LS Sbjct: 121 RSYLALVALQLTFWLALGEFARGMSGPHAQRYARWSFGRCIGIVSLLIFISTHVARFVLS 180 Query: 181 FSHPELFIPPAMSNIGVALIYLIPLSGSLLFSVSLMMIYFERLLADRQRLATEDQLTGAL 240 FSHPELFIPPAMSNIGVALIYLIPLSGSLLFSVSLMMIYFERLLADRQRLATEDQLTGAL Sbjct: 181 FSHPELFIPPAMSNIGVALIYLIPLSGSLLFSVSLMMIYFERLLADRQRLATEDQLTGAL 240 Query: 241 NRRELVRCGELLLARAVERKSVLALAFIDVDHFKQINDRHGHLAGDRVLAGIGATLAQSC 300 NRRELVRCGELLLARAVERKSVLALAFIDVDHFKQINDRHGHLAGDRVLAGIGATLAQSC Sbjct: 241 NRRELVRCGELLLARAVERKSVLALAFIDVDHFKQINDRHGHLAGDRVLAGIGATLAQSC 300 Query: 301 RPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLESVRTQRFEHGHPVTISVGLAVLQPG 360 RPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLESVRTQRFEHGHPVTISVGLAVLQPG Sbjct: 301 RPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLESVRTQRFEHGHPVTISVGLAVLQPG 360 Query: 361 NQRSWDELVHEADLALYRAKNEGRNAYRMAMSFGH 395 NQRSWDELVHEADLALYRAKNEGRNAYRMAMSFGH Sbjct: 361 NQRSWDELVHEADLALYRAKNEGRNAYRMAMSFGH 395 >gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767 [Herbaspirillum seropedicae SmR1] Length = 534 Score = 115 bits (287), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 64/159 (40%), Positives = 91/159 (57%), Gaps = 5/159 (3%) Query: 230 LATEDQLTGALNRRELVRCGELLLARAVERKSVLALAFIDVDHFKQINDRHGHLAGDRVL 289 L EDQLTG+LNRR + + + A+ R + L +A +D+D FK+IND HGH GD VL Sbjct: 363 LVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDEVL 422 Query: 290 AGIGATLAQSCRPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLESVRTQRFEHGHP-- 347 + + ++ R DV+ R+GGEEF + P A QI R+ + + F H Sbjct: 423 VHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIFMHESQRL 482 Query: 348 -VTISVGLAVLQPGNQRSWDELVHEADLALYRAKNEGRN 385 +T S G+AV PG S +L+ AD+ALY+AKN G+N Sbjct: 483 LITFSAGVAVYHPGE--SQADLLKRADVALYKAKNAGKN 519 >gi|300313993|ref|YP_003778085.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 66/178 (37%), Positives = 93/178 (52%), Gaps = 5/178 (2%) Query: 213 VSLMMIYFERLLADRQRLATEDQLTGALNRRELVRCGELLLARAVERKSVLALAFIDVDH 272 V L ER + L+ D LTG NRR + RAV LAL +DVD+ Sbjct: 384 VRLRTRELERANRKLEALSATDGLTGVANRRRFDEALANEMRRAVRSGQSLALLMLDVDY 443 Query: 273 FKQINDRHGHLAGDRVLAGIGATLAQSCRPG-DVLGRYGGEEFCAVFPGVDHDQACQIGQ 331 FK+ NDR+GHLAGD L + L +S R D++ RYGGEEF + D QA Q + Sbjct: 444 FKKYNDRYGHLAGDECLRIVARVLQKSSRRATDLVARYGGEEFSVIIAECDTTQALQQAE 503 Query: 332 RLLESVRTQRFEHGHP----VTISVGLAVLQPGNQRSWDELVHEADLALYRAKNEGRN 385 + +++ + H ++ S+G+AVL P + +++V AD ALY AK +GRN Sbjct: 504 NICQAIFELKLPHADSPFGYISASIGVAVLLPDEHTAPEQMVRIADQALYHAKYQGRN 561 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 72/173 (41%), Positives = 97/173 (56%), Gaps = 10/173 (5%) Query: 222 RLLADRQ--RLATEDQLTGALNRRELVRCGELLLARAVERK--SVLALAFIDVDHFKQIN 277 R+ A+RQ LA D LT LNRR L + R +R + ++ FIDVD+FK N Sbjct: 336 RVTAERQLEALARTDGLTDLLNRRALDEALQATWERC-QRNLGASFSVLFIDVDYFKAYN 394 Query: 278 DRHGHLAGDRVLAGIGATL-AQSCRPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLES 336 D +GH GD VL + ATL A+ R D GRYGGEEF + D A + QRL ++ Sbjct: 395 DTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGAQMMAQRLCDA 454 Query: 337 VRTQRFEHGHP----VTISVGLAVLQPGNQRSWDELVHEADLALYRAKNEGRN 385 VR + H VTISVG+A L+ + R +++++ AD ALYRAK GR+ Sbjct: 455 VRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKQAGRD 507 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 75/198 (37%), Positives = 110/198 (55%), Gaps = 16/198 (8%) Query: 199 LIYLIPLSGSLLFSVSLMMIYFERLLADRQ--RLATEDQLTGALNRRELVRCGELLLA-- 254 LI L+G LL V ++ +R+ A++Q LA D LTG LNRR L E LLA Sbjct: 295 LIIFTALAGVLLMLVRRELL--KRVAAEKQLEDLARTDGLTGLLNRRSL---DEALLAVW 349 Query: 255 RAVERKSVLALA--FIDVDHFKQINDRHGHLAGDRVLAGIGATLAQSC-RPGDVLGRYGG 311 + +R + FIDVD+FK+ ND +GH GD VL + A L + R D+ GRYGG Sbjct: 350 QRSQRSPTAGFSTLFIDVDYFKRYNDTYGHEMGDTVLQAVAAVLREHLQRQNDLAGRYGG 409 Query: 312 EEFCAVFPGVDHDQACQIGQRLLESVRTQRFEH-GHP---VTISVGLAVLQPGNQRSWDE 367 EEF + D A ++ Q+L E++ + H G P +++S+G+A + + ++ Sbjct: 410 EEFVVLLEQTDEHGALRMAQQLCEAIYNRGIPHSGSPLGVISVSIGVATMNRAPHQRMED 469 Query: 368 LVHEADLALYRAKNEGRN 385 +++ AD ALY AK EGRN Sbjct: 470 VLNAADHALYIAKREGRN 487 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,263,556 Number of extensions: 53320 Number of successful extensions: 283 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 39 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)