BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0005.p (564 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 1135 0.0 gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 171 1e-43 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 88 2e-18 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 1135 bits (2936), Expect = 0.0, Method: Compositional matrix adjust. Identities = 559/564 (99%), Positives = 560/564 (99%) Query: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQ 60 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAA Q Sbjct: 1 MPATVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQ 60 Query: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL Sbjct: 61 AKLTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELL 120 Query: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE Sbjct: 121 FRDVAAYSQIITSPDQAPAVFHQAIAQAYAQRGVAHLNIPADVIGAKTSGSVPSLHTLRE 180 Query: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLV 240 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDL Sbjct: 181 RTPLSPPAADIQAAADLINAAGSVAIFAGNGCRGAIDEVLALASKLQAPVMHTFRGKDLA 240 Query: 241 GYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 YDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR Sbjct: 241 SYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPYSEFLPTKSKVIQIDERGFVLGR 300 Query: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK Sbjct: 301 RAPVTLGITGSVAPAVQQLLERVEGKTDGAFLQQANAHRERWNAKLDSAAEIKRHHKKIK 360 Query: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI Sbjct: 361 PQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGTSLGQANGI 420 Query: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSF 480 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMP+F Sbjct: 421 QALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPAF 480 Query: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH Sbjct: 481 EGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAVLDVFIDPDELPVMPH 540 Query: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 IKMEQIWRFGLAKVRESLIAMKGG Sbjct: 541 IKMEQIWRFGLAKVRESLIAMKGG 564 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 171 bits (434), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 146/557 (26%), Positives = 240/557 (43%), Gaps = 24/557 (4%) Query: 4 TVADILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAVCQAKL 63 T ADIL+ L E GV +FG G A+ + DAI + + + I VRHE+ A AA ++ Sbjct: 7 TGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYSRS 66 Query: 64 TGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRD 123 + ++ V T+GPG + V GL A D P++ ISG VP+ G D QE + R Sbjct: 67 SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGITRP 126 Query: 124 VAAYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERT 182 ++ ++ +A A R G ++IP D+ + + P +R Sbjct: 127 CVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMRSYK 186 Query: 183 PLSP-PAADIQAAADLINAAGSVAIFAGNGC--RGAIDEVLALASKLQAPVMHTFRGKDL 239 P+ + I+ A L+ +A I+ G G A E+ L +L P +T G Sbjct: 187 PVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMGLGA 246 Query: 240 VGYDHPHWIGGVGLIGGAPGTDALHAAEVMLMLGSDYPY------SEFLPTKSKVIQIDE 293 ++G G+ G A+ +V++ +G+ + F K+I ID Sbjct: 247 YRSSSDKFVGMPGMHGTYEANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKIIHIDI 306 Query: 294 RGFVLGRRAPVTLGITGSVAPAVQQLLERV---EGKTDGAFL----QQANAHRERWNAKL 346 + +R V + I G+V +Q+LL ++ E + +GA L +Q N R+R K Sbjct: 307 DPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRDCLKF 366 Query: 347 DSAAEIKRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFN 406 ++ E IKPQSV + + ++ DA D G +W + +R + S Sbjct: 367 ATSEEF------IKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGG 420 Query: 407 NAAVGTSLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWG 466 +G L A G+Q + + V G+ M + E T ++ L K+++ NNR G Sbjct: 421 LGTMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLG 480 Query: 467 LV-HLEMEEAGMPSFEGSEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAAPGPAV 525 +V + + G E PDF A + G G + P +++G L++ A V Sbjct: 481 MVRQWQQIDYGSRYSESYMDSLPDFNKLAESYGHVGMKIEKPGDVDGALREAFAMKDRLV 540 Query: 526 LDVFIDPDELPVMPHIK 542 FI V P +K Sbjct: 541 FMNFITDQTENVWPMVK 557 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 87.8 bits (216), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 127/518 (24%), Positives = 220/518 (42%), Gaps = 61/518 (11%) Query: 49 HEEGAALAAVCQAK--LTGRLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAH 106 +E+ A +A+ AK L R+ + GPGA +L+ A + PVL + G V + Sbjct: 71 NEQAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFVSR 130 Query: 107 RGTDYLQE----NSPEL----LFRDVAAYSQIITSPDQAPAVFHQAIAQAY--AQRGVAH 156 R LQ+ N P L FR ++ I P+Q +AI AQ G Sbjct: 131 RPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGPVT 190 Query: 157 LNIPADVIGAKTSGSVPSLHTLRERTPL----SPPAADIQAAADLINAAGSVAIFAGNGC 212 L +P DV +T L R + +P A +++ A L+ A + AG G Sbjct: 191 LALPQDV---QTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQARQPLLIAGGGV 247 Query: 213 R--GAIDEVLALASKLQAPVMHTFRGKDLVGYDHPHWIGGVGLIGGAPGTDAL-HAAEVM 269 A + + A + PV T GK + + H +G +G + G+P +AL A+V+ Sbjct: 248 LYGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIG-VTGSPAANALAQEADVV 306 Query: 270 LMLGS---DYPY-SEFLPTKSKVIQIDERGFVLGRRAPVTLGITGSVAPAVQQLLERVEG 325 + +G+ D+ S L +++++ ++ + L + ++ L +EG Sbjct: 307 IAVGTRLQDFTTGSHTLFAQARLLNLNVNAMDAHKWR--GLALQADAGLGLEALSHALEG 364 Query: 326 -KTDGAFLQQANAHRERWNAKL-------DSAAEIKRHHKKIKPQSVARAISDLADDD-- 375 ++ + +A+ + W ++ D+ + + I ++ R+++D D Sbjct: 365 WRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIG--AIQRSVADSPSQDIV 422 Query: 376 --AAFVVDTGVVTLWCGNWIRQRGGQ--RILASFNNAAVGTSLGQANGIQALDRERQVVV 431 AA + + LW R G+ + + +G + G++ +R+V+V Sbjct: 423 VCAAGTLPAELHKLW-------RAGRPGAYHVEYGYSCMGYEVAGGLGVKLAQPQREVIV 475 Query: 432 AVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVHLEMEEAGMPSFE---------G 482 VGDG + M+ E TSV + VVV +NR +G ++ + G F G Sbjct: 476 IVGDGSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNMLADCLQAG 535 Query: 483 SEFPNPDFAAFARACGGQGFSVRTPEELEGGLQQLLAA 520 P DFAA AR+ G G +V+T ELE LQ+ AA Sbjct: 536 PGAPAIDFAAHARSLGALGENVKTITELEAALQRARAA 573 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,793,485 Number of extensions: 77656 Number of successful extensions: 243 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 4 Length of query: 564 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 474 Effective length of database: 1,193,720 Effective search space: 565823280 Effective search space used: 565823280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)