BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0050.p (530 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspiri... 1055 0.0 gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herb... 278 6e-76 >gi|300313170|ref|YP_003777262.1| altronate hydrolase [Herbaspirillum seropedicae SmR1] Length = 511 Score = 1055 bits (2729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 511/511 (100%), Positives = 511/511 (100%) Query: 20 MKAHSPVIRLNPVDDVVIARQQLISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKR 79 MKAHSPVIRLNPVDDVVIARQQLISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKR Sbjct: 1 MKAHSPVIRLNPVDDVVIARQQLISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKR 60 Query: 80 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPATFMGIVRPDGRV 139 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPATFMGIVRPDGRV Sbjct: 61 YGQIIGTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPATFMGIVRPDGRV 120 Query: 140 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDSQGE 199 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDSQGE Sbjct: 121 ATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDSQGE 180 Query: 200 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA 259 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA Sbjct: 181 PLQILRRTLAGYATHPNFAAVLVVGLGCETNQISGLMESHNLKEGEYFHTFTIQGTGGTA 240 Query: 260 KTVALGIEKIKKMLPKANDIKREPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 319 KTVALGIEKIKKMLPKANDIKREPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR Sbjct: 241 KTVALGIEKIKKMLPKANDIKREPVSAKHLTLGLQCGGSDGYSGITANPALGAAVDLLVR 300 Query: 320 HGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKNDAEMNNNPSAGNK 379 HGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKNDAEMNNNPSAGNK Sbjct: 301 HGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKNDAEMNNNPSAGNK 360 Query: 380 AGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYDPISATGQVAGGAN 439 AGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYDPISATGQVAGGAN Sbjct: 361 AGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYDPISATGQVAGGAN 420 Query: 440 MICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGNVTPQEIGERFFQM 499 MICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGNVTPQEIGERFFQM Sbjct: 421 MICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGNVTPQEIGERFFQM 480 Query: 500 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 530 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT Sbjct: 481 ILDTASGKKTKSELHGYGQDEFVPWHIGVYT 511 >gi|300312450|ref|YP_003776542.1| D-galactarate dehydratase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 278 bits (712), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 181/502 (36%), Positives = 265/502 (52%), Gaps = 37/502 (7%) Query: 27 IRLNPVDDVVIARQQ--LISGTVLQDEGGLKVQGLIPAGHKMATRAITAGEPVKRYGQII 84 I +N D+V I L +GTV D GL + +P GHK+A R + GE + RY I Sbjct: 16 IMMNDTDNVAIVVNDGGLPAGTVFPD--GLTLVDRVPQGHKIALRDLKQGEAIVRYDVAI 73 Query: 85 GTASQDIAPGQHVHTHNLAMAEFSREHHFGADVKPVDFVATPA--------TFMGIVRPD 136 G A +DI G + +L +RE D P+ PA TF G D Sbjct: 74 GYAVRDIPKGGWIE-ESLVQMPPARE----LDNLPI-ATKKPAPQPPLEGYTFEGYRNAD 127 Query: 137 GRVATRNYIGVLTSVNCSATAARAIADYFRRDIHPEVLADYPNIDGVVALTHGQGCALDS 196 G V TRN + + T+V C A + ++ + I E+L YPN++ VVAL H GC + Sbjct: 128 GSVGTRNLLAITTTVQCVA----GVVEHAVKRIRAELLPKYPNVEDVVALEHTYGCGVAI 183 Query: 197 QGEPLQILRRTLAGYATHPNFAA-VLVVGLGCETNQISGLMESHNL---KEGEYFHTFTI 252 I RTL + +PNF +VV LGCE Q + L+ + + K+GE + + Sbjct: 184 DAPNAGIPIRTLRNISLNPNFGGQAMVVSLGCEKLQPNRLLPENMIPIHKQGEPY-VVCL 242 Query: 253 QGTGGTAKTVALG--IEKIKKM----LPKANDIKREPVSAKHLTLGLQCGGSDGYSGITA 306 Q A+ V I+ I M L + N +RE A L +G+QCGGSD +SG+TA Sbjct: 243 Q----DAEHVGFNSMIDSIMNMAEARLTELNKRRRETCPASDLVVGVQCGGSDAFSGVTA 298 Query: 307 NPALGAAVDLLVRHGGTAILSETPEIYGAEHLLTRRAVSPEVGEKLLARIAWWEEYCAKN 366 NPA+G A DLLVR G + + SE E+ LT RAV+ EV + ++ + W++ Y + Sbjct: 299 NPAVGFATDLLVRAGASVMFSEVTEVRDGIDQLTSRAVNEEVAQAMIREMDWYDNYLKQG 358 Query: 367 DAEMNNNPSAGNKAGGLTTILEKSLGAVAKGGTTNLVDVYKYAEAVTARGFVFMDTPGYD 426 + + N + GNK GGL I+EK++G++ K G++ + V + + +G ++ TP D Sbjct: 359 GVDRSANTTPGNKKGGLANIVEKAMGSIVKSGSSPISGVLSPGDKLQQKGLIYAATPASD 418 Query: 427 PISATGQVAGGANMICFTTGRGSAYGCAPAPSLKLATNTALWQRQEEDMDINCGEIADGN 486 I T Q+A G N+ FTTGRG+ YG A P +K+AT L +R + MD+N G IA G Sbjct: 419 FICGTLQLAAGMNLHIFTTGRGTPYGLAAVPVIKVATRNDLARRWHDLMDVNAGRIASGE 478 Query: 487 VTPQEIGERFFQMILDTASGKK 508 + +++G FQ++LD ASGKK Sbjct: 479 ASIEDVGWELFQLMLDVASGKK 500 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,843,456 Number of extensions: 84293 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 2 Length of query: 530 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 440 Effective length of database: 1,193,720 Effective search space: 525236800 Effective search space used: 525236800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)