BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0037.p (701 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 1438 0.0 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 122 1e-28 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 117 3e-27 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 62 2e-10 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1438 bits (3722), Expect = 0.0, Method: Compositional matrix adjust. Identities = 695/701 (99%), Positives = 699/701 (99%) Query: 1 MTTTIVRAACPHDCPDTCALLVTVKDGVATEVRGDPAHPPTAGVLCTKVSRYAERTYHPD 60 MTTTIVRAACPHDCPDTCALLVTVKDGVATEVRGDPAHPPTAGVLCTKVSRYAERTYHPD Sbjct: 1 MTTTIVRAACPHDCPDTCALLVTVKDGVATEVRGDPAHPPTAGVLCTKVSRYAERTYHPD 60 Query: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES Sbjct: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 Query: 121 MAGRFFHRLGASQLERTICASAGGAGYRYTIGARIGADLEQVDHAKLILIWGGNPIASNL 180 MAGRFFHRLGASQLERTICASAGGAGYRYTIGAR+GADLEQVDHAKLILIWGGNPIASNL Sbjct: 121 MAGRFFHRLGASQLERTICASAGGAGYRYTIGARVGADLEQVDHAKLILIWGGNPIASNL 180 Query: 181 HFWTRVQTAKRNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALALGMMHVLISEDLLDH 240 HFWTRVQTAKRNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALALGMMHVLISEDLLDH Sbjct: 181 HFWTRVQTAKRNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALALGMMHVLISEDLLDH 240 Query: 241 DYIARHTLGFEQLRERVAEWTPARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRANYG 300 DYIARHTLGFEQLRERVAEWTPARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRANYG Sbjct: 241 DYIARHTLGFEQLRERVAEWTPARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRANYG 300 Query: 301 LQRVRGGGMAARNVACLPALVGAWRHPAGGVQMSVSDSFPKDAAALSRPDLAARHGKPTR 360 LQRVRGGGMAARNVACLPALVGAWRHPAGGVQMSVSDSFPKDAAALSRPDLAA HGKP R Sbjct: 301 LQRVRGGGMAARNVACLPALVGAWRHPAGGVQMSVSDSFPKDAAALSRPDLAAGHGKPAR 360 Query: 361 IINMSTIGDAVLHPASADFGPKVEALIVYNSNPVAVAPESAKVAQGFAREDLFTVVLEHF 420 +INMSTIGDA+LHPASADFGPKVEALIVYNSNPVAVAPESAKVAQGFAREDLFTVVLEHF Sbjct: 361 VINMSTIGDALLHPASADFGPKVEALIVYNSNPVAVAPESAKVAQGFAREDLFTVVLEHF 420 Query: 421 QTDTADYADILLPATTQLEHIDVHASYGHVYMMANNAAIAPMGEAKPNTEIFRRLAAAMG 480 QTDTADYADILLPATTQLEHIDVHASYGHVY+MANNAAIAPMGEAKPNTEIFRRLAAAMG Sbjct: 421 QTDTADYADILLPATTQLEHIDVHASYGHVYIMANNAAIAPMGEAKPNTEIFRRLAAAMG 480 Query: 481 FTEDCFQDSDDQLAAVAFKRDDPRSAGYDWEQLKQKGWQRLAVPVAPFAEGGFATPSGKC 540 FTEDCFQDSDDQLAAVAFKRDDPRSAGYDWEQLKQKGWQRLAVPVAPFAEGGFATPSGKC Sbjct: 481 FTEDCFQDSDDQLAAVAFKRDDPRSAGYDWEQLKQKGWQRLAVPVAPFAEGGFATPSGKC 540 Query: 541 EFYSEQMKAAGLDPLPAYIPPYESVASNPQLAARYPLAMISPPARNFLNSSFVNVQSLRD 600 EFYSEQMKAAGLDPLPAYIPPYESVASNPQLAARYPLAMISPPARNFLNSSFVNVQSLRD Sbjct: 541 EFYSEQMKAAGLDPLPAYIPPYESVASNPQLAARYPLAMISPPARNFLNSSFVNVQSLRD 600 Query: 601 TEGEPHLDMHPQDAAARGISQGDMVRIFNDRGSLQARVRVTDKARVGLVVGLSIWWKKFA 660 TEGEPHLDMHPQDAAARGISQGDMVRIFNDRGSLQARVRVTDKARVGLVVGLSIWWKKFA Sbjct: 601 TEGEPHLDMHPQDAAARGISQGDMVRIFNDRGSLQARVRVTDKARVGLVVGLSIWWKKFA 660 Query: 661 ADGKNANEVTSQRLTDMGHAPTFYDVLVQVEKLPAQGLLAG 701 ADGKNANEVTSQRLTDMGHAPTFYDVLVQVEKLPAQGLLAG Sbjct: 661 ADGKNANEVTSQRLTDMGHAPTFYDVLVQVEKLPAQGLLAG 701 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 122 bits (305), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 172/732 (23%), Positives = 297/732 (40%), Gaps = 74/732 (10%) Query: 10 CPHDCPDTCALLVTVKDGVATEVRGDPAHPPTAGVLCTKVSRYAERTYHPDRLLHPMRRV 69 CP+ C C + ++KD ++GD HP G LC K S E RLLHP R Sbjct: 19 CPY-CGVGCGVSASMKDDGRIGIQGDAQHPANFGRLCVKGSALGETVDLDGRLLHPQLR- 76 Query: 70 GKKGEGKFERISWDEAIATIAQRLGQI-ALRDPQAILPYSYAGTMGQVQGES--MAGRFF 126 +G+ ++++WD+A+ +A + I A P A+ Y GQ+ E +A + Sbjct: 77 --DAQGQLQQLAWDQALDHVASKFSAIIAEHGPDAVALY----VSGQLLTEDYYIANKLM 130 Query: 127 H-RLGASQLERT--ICASAGGAGYRYTIGAR-IGADLEQVDHAKLILIWGGNPIASNLHF 182 +G++ ++ +C S+ AG++ G + E ++ A L+++ G N + Sbjct: 131 KGYIGSANIDTNSRLCMSSAVAGHKRAFGEDLVPVCYEDLELADLVVLVGSNAAWCHPIL 190 Query: 183 WTRVQTAK--RNGARLIAIDPYRSLTAEKCHQHIALLPGTDAALALGMMHVLISEDLLDH 240 + R+ K R RL+ IDP R+ T E H+ + PGTD L G+M L L D+ Sbjct: 191 FQRIARLKETRPALRLVVIDPRRTATCELADLHLPIKPGTDVWLFNGLMSYLAHRGLRDN 250 Query: 241 DYIARHTLGF-EQLRERVAEWT-PARTAEVCGISAEEVLNLAREYGRVALAGEGVFIRAN 298 +I HT GF E L A+ PA A +C I +++L + E V + Sbjct: 251 AFIEAHTKGFDEALAVADADCADPATVARLCKIDLKDLLAFYEAFANT----EKVVTGFS 306 Query: 299 YGLQRVRGGGMAARNVACLPALVGAWRHPAGG---VQMSVSDSFPKDAAALSRPDLAARH 355 G+ + G ++ G P G + + ++ L+ ++ A H Sbjct: 307 MGVNQSSAGTDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLA--NMLAAH 364 Query: 356 GKPTRIINMSTIGDAVLHPASADF-------------GPKVEALIVYNSNPVAVAPESAK 402 + ++ + D P AD +++A+ + +NP+ P++ Sbjct: 365 MDLDQPLHRQIVQDFWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPMVSLPDADL 424 Query: 403 VAQGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHIDVHASYGHVYMMANNAAIAPM 462 V + + +L QTDT A +LLPA E D + + A ++ Sbjct: 425 VQRALQKCELVVSSDVITQTDTNALAHVLLPALAWGEK-DGTVTNSERRISRQRAFLSAP 483 Query: 463 GEAKPNTEIFRRLAAAMGFTEDCF---QDSDDQLAAVAFKRDDPRSA------------- 506 GEA+ + I +A MGF F + D+ A ++ R+ A Sbjct: 484 GEARGDWRIICDVARRMGFDGFDFAGPHEIFDEHARLSAHRNGEADASRVRRVFNLAGLT 543 Query: 507 GYD---WEQLKQKGWQRLAVPVAPFAEGGFATPSGKCEFYSEQMKAAGLDPLPAYIPPYE 563 G D ++ L W PV ++G + SG E + + D ++P Sbjct: 544 GMDRAAYDALAPIQW-----PVQRLSDG---SVSGTARVL-EDHRFSHADGRARFVPTAP 594 Query: 564 SVASNPQLAARYPLAMISPPARNFLNSSFVNVQS--LRDTEGEPHLDMHPQDAAARGISQ 621 A++ YPL + + R+ ++ +S L E +D+HPQDA G+ + Sbjct: 595 RSAAHAA-CEDYPLILNTGRVRDHWHTMTRTGRSATLASHIPESFIDIHPQDALLYGVRE 653 Query: 622 GDMVRIFNDRGSLQARVRVTDKARVGLVVGLSIWWKKFAADGKNANEVTSQRLTDMGHAP 681 G + RI + G++ ARV+ G V + I W +A V + + + P Sbjct: 654 GGLARISSRWGAMVARVQHGGGIARGSVF-VPIHWNGQSASDARVGAVVNPVVDPVSGEP 712 Query: 682 TFYDVLVQVEKL 693 F V +E+ Sbjct: 713 EFKHTPVAIEEF 724 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 117 bits (293), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 163/674 (24%), Positives = 260/674 (38%), Gaps = 88/674 (13%) Query: 58 HPDRLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALR---DPQAILPYSYAGTMG 114 H DR+L+PM R K + +SW+EA++ A +I + D + S Sbjct: 295 HKDRILNPMIR--KSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEE 352 Query: 115 QVQGESMAGRFFHRLGASQLERTICASAGGAGYRYTIGARIGAD-LEQVDHAKLILIWGG 173 + + F R +C S G G + T+G G E V + +ILI G Sbjct: 353 TYLVQKLVRAAFGNNNVDTCAR-VCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGA 411 Query: 174 NPIASNLHFWTRVQTAKRNGARLIAIDPYRSLTAEKCH----QHIALLPGTDAALALGMM 229 NP A + F ++++ R GA+LI IDP + + H H+ L PGT+ A+ + Sbjct: 412 NPAAGHPVFASQIKRRVRQGAKLIVIDPRTTEMVKSPHIQADYHLKLRPGTNTAIITALA 471 Query: 230 HVLISEDLLDHDYIARHT--LGFEQLRERV--AEWTPARTAEVCGISAEEVLNLAREYGR 285 HV++SE L D YI ++ +E V + +P A + G+ AE + AR Y Sbjct: 472 HVILSEGLQDEAYIQERCDLQSYQDWKEFVLREDNSPEAMAAITGVPAEIIRGAARLYA- 530 Query: 286 VALAGEGVFIRANYGLQRVRGGGMAARNVACLPALVGAWRHPAGGV-----QMSVSDSFP 340 G I G+ G +A L G GV Q +V S Sbjct: 531 ---TGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVGVNPLRGQNNVQGSC- 586 Query: 341 KDAAALSRPDLAARHGKPTRIIN-MSTIGDAVLHPA---------SADFGPKVEALIVYN 390 D + RH + + N L+P A + L Sbjct: 587 -DMGSFPHELPGYRHISDSTVRNQFEQAWGVTLNPEPGLRIPNMFDAALDGSFKGLYCEG 645 Query: 391 SNPVAVAPESAKVAQGFAREDLFTVVLEHFQTDTADYADILLPATTQLEHIDVHASYGHV 450 + V P + VA + VV + F +TA YA + LP ++ LE + Sbjct: 646 EDIVQSDPNTQHVAAALQAMECI-VVQDLFLNETAKYAHVFLPGSSFLEKDGTFTNAER- 703 Query: 451 YMMANNAAIAPMGEAKPNTEIFRRLAAAMGFTEDCFQDSD--DQLAAVAFKRDDPRSAGY 508 ++ + P K + E+ LA A+G+ S+ D++AA+ P G Sbjct: 704 -RISRVRKVMPPKAGKSDWEVTVALAEALGYPMPYNHPSEIMDEIAALT-----PSFHGV 757 Query: 509 DWEQLKQKGWQRLAVPVAPFAEGGFATP--------SGKCEFYSEQMKAAGLDPLPAYIP 560 +++L++ G + P A G TP GK +F + Q Sbjct: 758 SYDKLEKLG--SIQWPCNESAPEG--TPIMHVGSFIRGKGKFINTQY------------- 800 Query: 561 PYESVASNPQLAARYPLAMISPPARNFLNSSFVNVQSLRDTEGEPH----LDMHPQDAAA 616 A++ ++ RYPL + + L+ V Q+ R + H L++HP DA Sbjct: 801 ----FATDEKVTQRYPLILTTG---RILSQYNVGAQTRRTENSQWHSEDRLEIHPHDAED 853 Query: 617 RGISQGDMVRIFNDRGSLQARVRVTDKARVGLVVGLSIWWKKFAADGKNANEVTSQRLTD 676 RGI +GD V + + G R VT+K + G+V + F AN +T+ Sbjct: 854 RGIREGDWVGVESRAGQTVLRATVTEKVQPGVV------YTTFHFPESGANVITTDNSDW 907 Query: 677 MGHAPTFYDVLVQV 690 + P + VQV Sbjct: 908 ATNCPEYKVTAVQV 921 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 62.0 bits (149), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 68/268 (25%), Positives = 111/268 (41%), Gaps = 43/268 (16%) Query: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 RL HPM V K+++I W +A A +A+ L AL DP + Y + Sbjct: 111 RLTHPM--VYDAATDKYKKIEWADAFALMAKHLN--ALPDPD--MADFYVSGRASNEAAF 164 Query: 121 MAGRFFHRLGASQLE--RTICASAGGAGYRYTIGARIGADL-EQVDHAKLILIWGGNPIA 177 + F + G + +C G T+G G L E DH +L++G NP Sbjct: 165 LFQLFVRQYGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPAT 224 Query: 178 SNLHFWTRVQTAKRNGARLIAIDPYRSLTAEKC---HQHIALLP---------------G 219 ++ ++ A + GA ++AI+P + E+ + +L G Sbjct: 225 NHPRMMGELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLG 284 Query: 220 TDAALALGMMHVLI----------SEDLLDHDYIARHTLGFEQL--RERVAEWTPARTAE 267 D AL G++ I +E ++D +I HT GFE+ + R W AE Sbjct: 285 GDLALIKGVIKRTIELDDAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDI-VAE 343 Query: 268 VCGISAEEVLNLAREY--GRVALAGEGV 293 G+S E++ LA+ Y G+ +A G+ Sbjct: 344 -SGVSREDIEKLAQVYVKGKAVIATWGM 370 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,398,150 Number of extensions: 108660 Number of successful extensions: 345 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 342 Number of HSP's successfully gapped: 4 Length of query: 701 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 609 Effective length of database: 1,184,250 Effective search space: 721208250 Effective search space used: 721208250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)