BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0036.p (566 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 1170 0.0 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 169 6e-43 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 161 1e-40 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 142 9e-35 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 141 1e-34 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 1170 bits (3026), Expect = 0.0, Method: Compositional matrix adjust. Identities = 566/566 (100%), Positives = 566/566 (100%) Query: 1 MERFWLASYPAGVPAEIDPGRYGSLVQMIDEAFVQHADRQAYVCMGKYLSYRELDSLSRA 60 MERFWLASYPAGVPAEIDPGRYGSLVQMIDEAFVQHADRQAYVCMGKYLSYRELDSLSRA Sbjct: 1 MERFWLASYPAGVPAEIDPGRYGSLVQMIDEAFVQHADRQAYVCMGKYLSYRELDSLSRA 60 Query: 61 LGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSG 120 LGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSG Sbjct: 61 LGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSG 120 Query: 121 AEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLP 180 AEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLP Sbjct: 121 AEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLP 180 Query: 181 NAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEW 240 NAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEW Sbjct: 181 NAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEW 240 Query: 241 LTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKELAK 300 LTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKELAK Sbjct: 241 LTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKELAK 300 Query: 301 YKVNTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGY 360 YKVNTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGY Sbjct: 301 YKVNTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGY 360 Query: 361 GLSETSPIASANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPG 420 GLSETSPIASANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPG Sbjct: 361 GLSETSPIASANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPG 420 Query: 421 YWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQ 480 YWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQ Sbjct: 421 YWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQ 480 Query: 481 HPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYCKEQFTGYKKPKYIEFRDEL 540 HPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYCKEQFTGYKKPKYIEFRDEL Sbjct: 481 HPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYCKEQFTGYKKPKYIEFRDEL 540 Query: 541 PKTNVGKILRRELRDEPAKAQQKQAA 566 PKTNVGKILRRELRDEPAKAQQKQAA Sbjct: 541 PKTNVGKILRRELRDEPAKAQQKQAA 566 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 169 bits (427), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 137/519 (26%), Positives = 229/519 (44%), Gaps = 45/519 (8%) Query: 47 KYLSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPL 106 + + +RE+++ AL L G++KG ++ + N Q + A + G V N Sbjct: 61 RLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFR 120 Query: 107 YTARELEHQLKDSGAEAIFVLENFACTLQKVVAHT-NIKHIVVASMGELLGALKGTLVNL 165 T E + + SGA + FA + V A + ++H+V L A G L Sbjct: 121 ITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVA-----LADARAGEL--- 172 Query: 166 VVRHVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVL 225 + + + G + + +DD + YT GTTG K +L Sbjct: 173 ------------------DYEALVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGML 214 Query: 226 THRNLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLI 285 +H + V L P L Q + PL H V ++ T GA ++L+ Sbjct: 215 SHGQMAFVVTNHLADLMPGL-----THQSRSLVVAPLSH--GAGVHAVINTARGAASILL 267 Query: 286 PNPRDIPGFVKELA-KYKVNTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSV 344 + R + +L KY+V+ V T+ L+ + R D S + + G + ++ Sbjct: 268 SSERLVVEEAWQLVEKYRVDNLFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRAD 327 Query: 345 ADKWKKLTGCPIIEGYGLSETSPIASANPCTI--------DEYTGTIGLPLPSTEFAILD 396 K G +++ YGL E + + P + GT G+P + AIL+ Sbjct: 328 QVYALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILN 387 Query: 397 DDGQPVPLGQPGEIAIRGPQVMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVD 456 DDGQ + + GEI +RGP V GY P+ AK D +F TGD+G +D+ G+ I Sbjct: 388 DDGQELAPFETGEICVRGPAVFMGYHNNPEANAKAFKHD-WFHTGDLGHVDQDGFLYITG 446 Query: 457 RKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEA-VKLFVVRKDKTLSAA 515 R DM + G NVYP E+E + HP V E A +GVPD GE+ + + V + + + Sbjct: 447 RSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGE 506 Query: 516 QLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 L+ + + + YK P+ + D +PK+ GKI++++++ Sbjct: 507 ALLAHLESRIGKYKWPRRFIYWDSMPKSGYGKIVKKQIK 545 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 161 bits (408), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 163/574 (28%), Positives = 252/574 (43%), Gaps = 66/574 (11%) Query: 7 ASYPAGVPA--EIDPGRYGSLVQMIDEAFVQHADRQAYVCMG-----KYLSYRELDSLSR 59 A Y AG P+ E + ++ Q + + + R A +C SY +L LS Sbjct: 14 AHYRAGNPSPYEWQIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQLKILSD 73 Query: 60 ALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDS 119 L+ G+ +G R+ I + ++ I A + G V + L+ + ++L +S Sbjct: 74 RFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNS 133 Query: 120 GAEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSL 179 GA A+ T+ + A++ L AL+ LV V LV L Sbjct: 134 GAVALV---------------TDASGMEKATLAGELPALR--LVFCV--ESSDLV----L 170 Query: 180 PNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANV----- 234 P F L A + L PV DD A + YT GTTG AKGA+ HR L+ ++ Sbjct: 171 PQTTDFWDRLHAAPAE-LDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEV 229 Query: 235 ------LQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLLIPNP 288 + + + TPA A I L+ V LYH A+ V L + A Sbjct: 230 SHDSFPQEGDRFWTPADWAW--IGGLLDVLLPSLYHGVAV-VARRLEKFDAA-------- 278 Query: 289 RDIPGFVKELAKYKVNTFPAVNTLYNALLNNADF-ARLDFSGYRCCVGGGMAVQKSVADK 347 ++ G LA++++ T L A A+ DFS R GG + + Sbjct: 279 -EVFGL---LARHQIRNVFFPPTALKMLRGAATVRAQADFS-LRSVASGGETLGDDLIAW 333 Query: 348 WKKLTGCPIIEGYGLSETSPIASANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQP 407 ++ G I E YG +E + + S++ +G++G +P I+D+ GQ +P G Sbjct: 334 GREALGVTINEFYGQTECNLVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTV 393 Query: 408 GEIAIRGPQ--VMPGYWQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVS 465 G IAIR P + YW+ + T + D F TGD+G MDE+GY + + R D+I + Sbjct: 394 GNIAIRAPDPVMFLRYWRNEEATREKFAGD-FLLTGDLGSMDEQGYIRYLGRNDDVITSA 452 Query: 466 GFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKLFVVRKDKTLS----AAQLMDYC 521 G+ + P +E + +HP V A VGV D E VK FVV KD A+L + Sbjct: 453 GYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKAELQQHV 512 Query: 522 KEQFTGYKKPKYIEFRDELPKTNVGKILRRELRD 555 + Q ++ P+ I F LP T GKI+R+ L++ Sbjct: 513 RAQLAAHEYPRLISFVAALPTTATGKIMRKTLKE 546 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 142 bits (357), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 152/565 (26%), Positives = 249/565 (44%), Gaps = 59/565 (10%) Query: 19 PGRYGS--LVQMIDEAFVQHADRQAYVCMGKYLSYRELDSLSRALGGWLQGLGLQKGARV 76 P YG L++ + + + A Q + + LSY + L L+ +GLQ G V Sbjct: 9 PSAYGYPLLIKQLLHSALATASGQEIIYGQRRLSYAGFYERVQRLANALKEMGLQPGNTV 68 Query: 77 AIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSGAEAIFVLENFACTLQK 136 A+M + +Y A+ G + VN + ++ + L + A+ + V +F L++ Sbjct: 69 AVMDWDSNRYLECFFAVPMMGCVLQTVNVRLSPEQIAYTLNHAQADVLLVNSDFLPLLKQ 128 Query: 137 VVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLPN-AITFSQALSQARGK 195 + GEL T + V P P A + + L+QA Sbjct: 129 I-------------RGEL------TTLTRCVWLTDDATPCPRGPGFAGEYEELLAQAEPA 169 Query: 196 TLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPALKAGKPIDQLV 255 P + A YT GTTG+ KG +HR LV + L L A + G+ V Sbjct: 170 CDFP-DFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLATTAGLALAPQQGRFHRDDV 228 Query: 256 FVCALPLYHIFAL---TVCNMLGTREGALNLLIPNPRDIPGFVKELAKYKVNTFP-AVNT 311 ++ P++H+ A V M G ++ + P R +P + L + + TF V T Sbjct: 229 YMPLTPMFHVHAWGLPYVATMAGVKQ-----VYPG-RYVPDTICRLIREEKVTFSHCVPT 282 Query: 312 LYNALLNNADFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASA 371 + +L + D SG++ +GG A+ S+A K + G + GYG+SET P+ + Sbjct: 283 ILQMVLTSEQAREADLSGWKMIIGGS-AMTGSLA-KMARDRGIDVFTGYGMSETCPVLTL 340 Query: 372 NPCTIDEYTG--------TIGLPLPSTEFAILDDDGQPVPL--GQPGEIAIRGPQVMPGY 421 +E G G PLP + +D + Q P GE+ +R P + GY Sbjct: 341 AQVPTEELGGEREAAIRVKTGRPLPLVDLRTVDGEMQDCPRDGSTTGEVVVRAPWLTQGY 400 Query: 422 WQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQH 481 + E ++ + G+ T DIG +D GY ++ DR KD+I G V ++E ++A+H Sbjct: 401 LHQA-EASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTGGEWVSSLQIEDIIARH 459 Query: 482 PGVLECACVGVPDEHTGEA-VKLFVVRKDKTLSAAQLMDYCKEQ---------FTGYKKP 531 P V E A +G+ D GE V L V++KD +AA D +E + Y P Sbjct: 460 PAVAETAVIGIQDGKWGERPVALVVLKKD---AAAVSEDDIREHVRAAAEAGAISRYGVP 516 Query: 532 KYIEFRDELPKTNVGKILRRELRDE 556 + F EL +T+VGK+ +R +R++ Sbjct: 517 DRVRFVAELARTSVGKLNKRVMREQ 541 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 141 bits (356), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 147/537 (27%), Positives = 230/537 (42%), Gaps = 49/537 (9%) Query: 49 LSYRELDSLSRALGGWLQGLGLQKGARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYT 108 +SYREL L+ LG++KG RV I MP ++ A+ A R G T V ++ Sbjct: 114 VSYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFS 173 Query: 109 ARELEHQLKDSGAEAIFVLENFACTLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVR 168 A+ L ++ D+GA A+ + +++ +K IV ++ +G TL ++VV Sbjct: 174 AKSLHERVIDAGAVAVLTADYQVRGGKQL----PLKSIVDEALD--MGGCD-TLKHVVVY 226 Query: 169 HVKKLVPAWSLPNAITFSQALSQARGKTLQPVTLTHDDIAFLQYTGGTTGVAKGAVLTHR 228 W + S ++ + T +P + + F+ YT G+TG KG Sbjct: 227 KRAGAEINWVEGRDLWLSDVVAN-QSDTCEPEWVDAEHPLFILYTSGSTGKPKGVQHASA 285 Query: 229 NLVANVLQAEEWLTPALKAGKPIDQLVFVCALPLYHIFALTVCNMLGTREGALNLL---I 285 + + +W KP D V+ C + I T GA ++ + Sbjct: 286 GYLLWAVLTMKWTFDI----KPDD--VYWCTADIGWITGHTYIAYGPLAVGATQIVFEGV 339 Query: 286 PNPRDIPGFVKELAKYKVNTFPAVNTLYNALLNNADF------ARLDFSGYRCCVGGGMA 339 P + F +A++K F T +L+ AD + D S R G Sbjct: 340 PTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEP 399 Query: 340 VQKSVADKWKKLTG---CPIIEGYGLSET-----SPIASANPCTIDEYTGTIGLPLPSTE 391 + + K G CPI++ + +ET SP+ A P + G+ LPLP Sbjct: 400 INPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGATP----QVPGSCTLPLPGIT 455 Query: 392 FAILDDDGQPVPLGQPGEIAIR--GPQVMPGYWQRPDETAKVMTPD----GFFKTGDIGV 445 AI+D+ G +P G G + ++ P ++ W P+ K P+ + GD V Sbjct: 456 AAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAV 515 Query: 446 MD-ERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQHPGVLECACVGVPDEHTGEAVKLF 504 + E GY I R D++ VSG + E+E + + V E A VG PDE TGE++ F Sbjct: 516 RNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAF 575 Query: 505 VVRK-------DKTLSAAQLMDYCKEQFTGYKKPKYIEFRDELPKTNVGKILRRELR 554 VV K + A +L D+ ++ KPK I F D LPKT GKI+RR LR Sbjct: 576 VVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLR 632 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,759,827 Number of extensions: 73992 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 19 Length of query: 566 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 476 Effective length of database: 1,193,720 Effective search space: 568210720 Effective search space used: 568210720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)