BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0035.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 635 0.0 gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid... 233 1e-62 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 220 1e-58 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 216 2e-57 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 181 8e-47 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 635 bits (1637), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/316 (99%), Positives = 316/316 (100%) Query: 1 MTTNNRPHVLIVARLPQHLLDLLQQHYTCHNLILQPHSEAELAAIAPQIRGIAANGEAKV 60 MTTNNRPHVLIVARLPQHLLDLLQQHYTCHNLILQPHSEAELAAIAPQIRGIAANGEAKV Sbjct: 1 MTTNNRPHVLIVARLPQHLLDLLQQHYTCHNLILQPHSEAELAAIAPQIRGIAANGEAKV 60 Query: 61 SREFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAIALMLATAR 120 SREFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLA+ALMLATAR Sbjct: 61 SREFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATAR 120 Query: 121 NVVRADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR 180 NVVRADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR Sbjct: 121 NVVRADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR 180 Query: 181 KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVDE 240 KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVDE Sbjct: 181 KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVDE 240 Query: 241 AALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMADLAFNN 300 AALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMADLAFNN Sbjct: 241 AALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMADLAFNN 300 Query: 301 LQAHFSGAGVISPVPD 316 LQAHFSGAGVISPVPD Sbjct: 301 LQAHFSGAGVISPVPD 316 >gi|300309865|ref|YP_003773957.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 348 Score = 233 bits (595), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 130/292 (44%), Positives = 180/292 (61%), Gaps = 6/292 (2%) Query: 21 DLLQQHYTCHNLILQPHSEAELAAIAPQIRGIAANGEAKVSREFMARFPALEIVSVFGVG 80 D L+ Y H L Q EA +A I G+ G +S M PAL++V+V GVG Sbjct: 49 DKLESLYHMHRLFEQEDKEAYIARHGAVIEGVITGGHTGISNALMDSLPALKVVAVNGVG 108 Query: 81 YDGVDVPAARERGIHVTHTPDVLNDDVADLAIALMLATARNVVRADRFARSGEWKKGP-- 138 D VD+ AR +G+ VT T L +DVADLA+ LMLA +R + F + G+W + P Sbjct: 109 TDAVDLNHARSKGLPVTGTFGALTEDVADLALGLMLAVSRQICPGHAFVQRGDWVRHPSP 168 Query: 139 --FPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHN-RSRKDVPYTYVDSITALA 195 P + ++SG R+GIVG+G++G+AIA+RAAAF I+Y + R+ +D+PY + + LA Sbjct: 169 TAIPLSRRLSGKRVGIVGMGKVGRAIAQRAAAFGCPITYTDLRAMEDLPYAFQPELLQLA 228 Query: 196 REVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVDEAALIAALKTGVIAGA 255 RE D LV+ + ++NA VL+ALG +G LIN+ARG +V E L+ AL+ GVIAGA Sbjct: 229 RESDVLVL-AAAADKAQGIINAAVLDALGREGILINIARGKLVVEEDLVDALQRGVIAGA 287 Query: 256 GLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMADLAFNNLQAHFSG 307 GLDVF DEPNVPA L +D VVL H AS T+ +RTAM ++ +L +G Sbjct: 288 GLDVFVDEPNVPAALLQMDQVVLQAHRASATVESRTAMGEMVLASLAQGLAG 339 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 220 bits (561), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 127/307 (41%), Positives = 176/307 (57%), Gaps = 8/307 (2%) Query: 6 RPHVLIVARLPQHLLDLLQQHYTCHNLILQPHSEAELAAIAPQIRGIA---ANGEAKVSR 62 +P +L++ L + + HY +++ P + IA R +A NG ++ Sbjct: 2 KPPLLVLIHLSEESTATIAAHY---DILYAPDRQRRDEMIAGPARKVAVVLTNGSTGLTA 58 Query: 63 EFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAIALMLATARNV 122 E M P L++V G G++ VDV A GI + D VAD A+ L+LA RN+ Sbjct: 59 EEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILRNI 118 Query: 123 VRADRFARSGEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRS-RK 181 DR+ R G W++ P +++G R+GIVG+G IG+ IA+RAAAFD +I+Y NR R Sbjct: 119 PVLDRYTRDGGWRE-TIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKKRD 177 Query: 182 DVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVVDEA 241 DV Y Y + LA D L++ PGGA TR L+NA VLE LGP+G+L+N+ RGS+VD Sbjct: 178 DVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGSIVDTD 237 Query: 242 ALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMADLAFNNL 301 AL AAL +G +AGAGLDV+ EP PA L AL NVVLTPH+A + A N Sbjct: 238 ALGAALSSGRLAGAGLDVYEGEPQPPAALIALPNVVLTPHIAGWSPEAIRASVTQFLRNC 297 Query: 302 QAHFSGA 308 + HF+ A Sbjct: 298 EEHFAAA 304 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 216 bits (549), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 129/312 (41%), Positives = 178/312 (57%), Gaps = 11/312 (3%) Query: 6 RPHVLIVARLPQHLLDLLQQHYTCH-NLILQPHSEAELAAIAPQIRGIAANGEAKVSREF 64 +P + + + +L L+QH+ N + +S AEL A A G+ V+ Sbjct: 2 KPKIFVARAVFPEVLARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPVTAAL 61 Query: 65 MARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAIALMLATARNVVR 124 A P L+ V VGY+ +DV AA + G+ T+TPDVLN+ AD ALM+A AR + Sbjct: 62 FAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRITE 121 Query: 125 ADRFARSGEWKKGPFP--FTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRSR-- 180 ++ F R+G+WKK + + GA LGI+G+GRIGQAIA+R+ FDMQ+ YHNRSR Sbjct: 122 SEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVLYHNRSRLA 181 Query: 181 -----KDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 + YV S L R+ D ++++ P T + A L + P L N+ARG Sbjct: 182 PELEARANHARYV-SKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARG 240 Query: 236 SVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALDNVVLTPHMASGTLVTRTAMAD 295 +VD+AALIAAL+ G IA AG+DVF +EP E L NVVLTPH+AS + TR AMA+ Sbjct: 241 GIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMAN 300 Query: 296 LAFNNLQAHFSG 307 A +NL A SG Sbjct: 301 CAADNLIAALSG 312 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 181 bits (459), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 122/316 (38%), Positives = 170/316 (53%), Gaps = 14/316 (4%) Query: 6 RPHVLIVARLPQHLLDLLQQHY--TCHNLILQPHSEAELAAIAPQIRGIAANGEAKVSRE 63 +PHV++ +LP+HL+ LQ + T + I + +AA+A I A+ ++ Sbjct: 2 KPHVVVYKKLPEHLVQRLQAEFDLTLFDRITDDNRADFIAALATAHGMIGAS--LPITNA 59 Query: 64 FMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAIALMLATARNVV 123 + P L+IVS VG D D+ R+RG+ + HTP VL + AD AL+LA+AR VV Sbjct: 60 MLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRVV 119 Query: 124 RADRFARSGEWKK--GPFPFTTKVSGARLGIVGLGRIGQAIAKRA-AAFDMQISYHNR-- 178 + ++G WK G F V G LG++G+GRIG A+A+RA F M I YHNR Sbjct: 120 ELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRP 179 Query: 179 ---SRKDVPYTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARG 235 + +++ YV LA + DF+ ++ P T + A + IN +RG Sbjct: 180 DPEAERELGARYVSKEDLLA-QADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRG 238 Query: 236 SVVDEAALIAALKTGVIAGAGLDVFADEP-NVPAELAALDNVVLTPHMASGTLVTRTAMA 294 +VDEAALIAAL+ I GAGLDVF EP + + L L NVV PH+ S T TR AMA Sbjct: 239 RIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMA 298 Query: 295 DLAFNNLQAHFSGAGV 310 +LA NL A G V Sbjct: 299 ELAVTNLIAGLRGEPV 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 965,691 Number of extensions: 41100 Number of successful extensions: 182 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 11 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)