BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0015.p (427 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-ami... 869 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 148 6e-37 gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 126 3e-30 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 119 4e-28 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 108 1e-24 >gi|300313138|ref|YP_003777230.1| glutamate-1-semialdhyde 2,1-aminomutase [Herbaspirillum seropedicae SmR1] Length = 427 Score = 869 bits (2246), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/427 (100%), Positives = 427/427 (100%) Query: 1 MTKTNASLFARAQQSTPGGVNSPVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSW 60 MTKTNASLFARAQQSTPGGVNSPVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSW Sbjct: 1 MTKTNASLFARAQQSTPGGVNSPVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSW 60 Query: 61 GPAIVGHAHPEVIQAVQQAAARGLSFGAPTEAEIEMAEEIIKLVPSIEQIRLVSSGTEAT 120 GPAIVGHAHPEVIQAVQQAAARGLSFGAPTEAEIEMAEEIIKLVPSIEQIRLVSSGTEAT Sbjct: 61 GPAIVGHAHPEVIQAVQQAAARGLSFGAPTEAEIEMAEEIIKLVPSIEQIRLVSSGTEAT 120 Query: 121 MSALRLARGATGRDKIIKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVL 180 MSALRLARGATGRDKIIKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVL Sbjct: 121 MSALRLARGATGRDKIIKFEGCYHGHADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVL 180 Query: 181 DYNDAAQLEEAFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEV 240 DYNDAAQLEEAFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEV Sbjct: 181 DYNDAAQLEEAFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEV 240 Query: 241 MSGFRVARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLS 300 MSGFRVARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLS Sbjct: 241 MSGFRVARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLS 300 Query: 301 GNPVTVAAGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQAAGVTFAADAIGGMFGLYF 360 GNPVTVAAGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQAAGVTFAADAIGGMFGLYF Sbjct: 301 GNPVTVAAGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQAAGVTFAADAIGGMFGLYF 360 Query: 361 DAKVPTSYAEVMQGDKERFNRFFHKMLDAGVYFAPSAFEAGFVSAQHSDAIIEETVAAAA 420 DAKVPTSYAEVMQGDKERFNRFFHKMLDAGVYFAPSAFEAGFVSAQHSDAIIEETVAAAA Sbjct: 361 DAKVPTSYAEVMQGDKERFNRFFHKMLDAGVYFAPSAFEAGFVSAQHSDAIIEETVAAAA 420 Query: 421 RAFSELG 427 RAFSELG Sbjct: 421 RAFSELG 427 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 148 bits (374), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 109/332 (32%), Positives = 170/332 (51%), Gaps = 33/332 (9%) Query: 28 RSVGGTPR--------FIERAEGPWFWDAEGKRYIDYIGSWGPAIVGHAHPEVIQAVQQA 79 R TPR + ERA WD EG+R+ID+ GH HP+++ A++ Sbjct: 13 RKNAATPRGVGVMCDFYAERAANAELWDVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQ 72 Query: 80 AARGLSFG---APTEAEIEMAEEIIKLVPS--IEQIRLVSSGTEATMSALRLARGATGRD 134 + P + +E+AE I +L P ++ S+G EA +A+++AR TGR Sbjct: 73 MDKFTHTAYQIVPYASYVELAERINRLTPGNYPKKTAFFSTGAEAVENAIKIARAHTGRP 132 Query: 135 KIIKFEGCYHGHAD---SLLVKAGSGLLTFGNPTSAGV---PEDFAKHTLVLDYNDAAQ- 187 +I F G +HG +L K L FG P V P A H + + DA + Sbjct: 133 GVIAFAGGFHGRTMMGMALTGKVAPYKLGFG-PFPGDVFHAPYPSALHGITSE--DALEA 189 Query: 188 LEEAFKT--AGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEVMSGF- 244 ++ FK+ +A +I+EPV G A +F+R +R LC E+G +LI DEV SG+ Sbjct: 190 VKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYG 249 Query: 245 RVARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLSGNPV 304 R + A E ++PDL + K + GG+P++A GRAE+M AP GG+ GT +GNP+ Sbjct: 250 RTGKLFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIMDAPAP-GGL--GGTYAGNPL 306 Query: 305 TVAAGMATLKIIQQPDFYTNLSTQTRKLADGL 336 +A+ +A L ++++ L T+ ++L D L Sbjct: 307 AIASALAVLDVMEE----EQLVTRGQRLGDKL 334 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 126 bits (317), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 97/305 (31%), Positives = 147/305 (48%), Gaps = 31/305 (10%) Query: 42 GPWFWDAEGKRYIDYIGSWGPAIVGHAHPEVIQAVQQAAARGLSFGAP---TEAEIEMAE 98 G W D GKRY+DY+ W +GHA P+ I A ++ L +P E IE+A+ Sbjct: 27 GMWLTDHNGKRYLDYLQGWAVNTLGHA-PQCIADALAAQSKKLINPSPAFYNEPSIELAK 85 Query: 99 EIIKLVPSIEQIRLVSSGTEATMSALRLAR---------GATGRDKIIKFEGCYHGHADS 149 ++ +++ +SG EA A++LAR + R +II F+ +HG + Sbjct: 86 -LLTANSVFDRVFFANSGGEANEGAIKLARKWGKKNPAADGSARFEIITFKHSFHGRTLA 144 Query: 150 LLVKAGS-GLLTFGNPTSAGVPEDFAKHTLVLDYNDAAQLEEAFKTAGNEIACVIVEPVA 208 + +G G T P G P+ ND LE G V++EPV Sbjct: 145 TMSASGKDGWDTMFAPQVPGFPKAV--------LND---LESVKALIGEHTVAVMLEPVQ 193 Query: 209 GNMNLVRASDEFLRTMRRLCTEYGAILIFDEVMSGF-RVARGGAQELNGIVPDLTALGKV 267 G ++ AS EF++ +R L E +LI DEV SG R + A + +GI PD+ L K Sbjct: 194 GEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQHSGIEPDIMTLAKG 253 Query: 268 IGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLSGNPVTVAAGMATLKIIQQPDFYTNLST 327 IGGG+P+AA R E+ A Q GT +GNP+ A G+A +K + +P F ++ Sbjct: 254 IGGGVPLAALLAREEI----ACFEAGEQGGTYNGNPLMTAVGVAVIKELLKPGFMESVRE 309 Query: 328 QTRKL 332 + + L Sbjct: 310 RGQYL 314 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 119 bits (298), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 96/314 (30%), Positives = 156/314 (49%), Gaps = 28/314 (8%) Query: 28 RSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSWGPAIVGHAHPEVIQAV-QQAAARGLSF 86 R G P +++A G F D +G +ID G + VG +P V++A+ +Q+ + S Sbjct: 42 RGGGRMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSM 101 Query: 87 GAPTEAEIEMAEEIIKLVPSIEQIR------LVSSGTEATMSALRLARGATGRDKIIKFE 140 + E+A +I +++P + +R G++A +A++ A+ TGR +II F Sbjct: 102 EVNSSKRTELAAKISEIMP--DGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFH 159 Query: 141 GCYHG--HADSLLVKAGSGLLTFGNPTSAGV---PEDFA-KHTLVLDYNDAAQLEEAF-- 192 G YHG +A + L + FG P GV P +A + + A Q+ + Sbjct: 160 GGYHGIWNASNALTTGTAYRKGFG-PFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYVD 218 Query: 193 ------KTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILIFDEVMSGF-R 245 TA +++A VIVEPV G V S EFL+ +R+ C GA+LI DEV +G R Sbjct: 219 YLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGR 278 Query: 246 VARGGAQELNGIVPDLTALGKVIGGGLPVAAFGGRAEVMKHLAPLGGVYQAGTLSGNPVT 305 + A E +G+ PD+ GK IGG +P+A R+++ + P G Q T + N ++ Sbjct: 279 TGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKI-PDGS--QPNTFAANSIS 335 Query: 306 VAAGMATLKIIQQP 319 A + + I+Q P Sbjct: 336 AAVALTNISILQDP 349 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 108 bits (269), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 105/353 (29%), Positives = 162/353 (45%), Gaps = 42/353 (11%) Query: 23 PVRAFRSVGGTPRFIERAEGPWFWDAEGKRYIDYIGSWGPAIVGHAHPEVIQAVQQAAAR 82 P A R+ PR + AEG + D G +D +GHAHP+++ AVQ++ A Sbjct: 18 PFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVAT 77 Query: 83 ---GLSFGAPTEAEIEMAEEIIKLVPS-IEQIRLVSSGTEATMSALRLA------RGATG 132 SF + E+AE + + + SG+EA +AL++A +G Sbjct: 78 LDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNAT 137 Query: 133 RDKIIKFEGCYHG--HADSLLVKAGSGLLTFGNPTSAGVPEDFAKHTLVLDYND------ 184 R ++I E YHG + G TF GV D HT L+ N Sbjct: 138 RTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGV--DHLPHTHNLEKNAFTKGEP 195 Query: 185 ------AAQLEE--AFKTAGNEIACVIVEPVAGNMNLVRASDEFLRTMRRLCTEYGAILI 236 A +LE A N IA VIVEPVAG+ ++ +L+ +R +CT++G +LI Sbjct: 196 EYGAHLADELERLVTLHDASN-IAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLI 254 Query: 237 FDEVMSGF-RVARGGAQELNGIVPDLTALGK-VIGGGLPVAAFGGRAEVMKHLAPLGG-- 292 FDEV++GF R+ A + + PD+ K + G +P+ A + E+ H A + G Sbjct: 255 FDEVITGFGRLGTPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEI--HDAFMNGPD 312 Query: 293 ---VYQAGTLSGNPVTVAAGMATLKIIQQPDFYTNLSTQTRKLADGLAAAAQA 342 ++ T SG+P+ AAG+A+L++ Q N + +AD AA A Sbjct: 313 GIELFHGYTYSGHPLACAAGLASLEVFQTEGVLEN----AQAVADYFQEAAHA 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,310,628 Number of extensions: 56288 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 10 Length of query: 427 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 339 Effective length of database: 1,203,190 Effective search space: 407881410 Effective search space used: 407881410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)