BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0010.p (697 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313134|ref|YP_003777226.1| twitching motility transmembran... 1389 0.0 gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis tra... 114 4e-26 gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis dom... 111 2e-25 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 110 3e-25 gi|300313877|ref|YP_003777969.1| methyl-accepting chemotaxis sen... 108 1e-24 >gi|300313134|ref|YP_003777226.1| twitching motility transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 1389 bits (3594), Expect = 0.0, Method: Compositional matrix adjust. Identities = 696/697 (99%), Positives = 696/697 (99%) Query: 1 MRLPYLHRFALRTQTRILLGILALSLAGLFLFLWLGSRLYIHATLQTHIASDLVMHSQRL 60 MRLPYLHRFALRTQTRILLGILALSLAGLFLFLWLGSRLYIHATLQTHIASDLVMHSQRL Sbjct: 57 MRLPYLHRFALRTQTRILLGILALSLAGLFLFLWLGSRLYIHATLQTHIASDLVMHSQRL 116 Query: 61 GKAVPNAIQGNPEAFVQLAQSRVEFNRGLLLLTSGGTYQGNTIGLPEPAMKPALESITRL 120 GKAVPNAIQGNPEAFVQLAQSRVEFNRGLLLLTSGGTYQGNTIGLPEPAMKPALESITRL Sbjct: 117 GKAVPNAIQGNPEAFVQLAQSRVEFNRGLLLLTSGGTYQGNTIGLPEPAMKPALESITRL 176 Query: 121 WRRSDLAAENILALQKELTGFGATLKRLNQLLPVMLDLSEQISMLQSQSRPTPREMNAAS 180 WRRSDLAAENILALQKELTGFGATLKRLNQLLPVMLDLSEQISMLQSQSRPTPREMNAAS Sbjct: 177 WRRSDLAAENILALQKELTGFGATLKRLNQLLPVMLDLSEQISMLQSQSRPTPREMNAAS 236 Query: 181 QLVMLTLRLGRSANEFLTSEGVNPETAFMLGKDITAFGDQLTSLRDGNEMLRLRPASDPD 240 QLVMLTLRLGRSANEFLTSEGVNPETAFMLGKDITAFGDQLTSLRDGNEMLRLRPASDPD Sbjct: 237 QLVMLTLRLGRSANEFLTSEGVNPETAFMLGKDITAFGDQLTSLRDGNEMLRLRPASDPD 296 Query: 241 LREKLDELERVFAQYQPQIAGILGHLQNFIAAKQAERLVFVENETLKRQLTELQDDYRIA 300 LREKLDELERVFAQYQPQIAGILGHLQNFIAAKQAERLVFVENETLKRQLTELQDDYRIA Sbjct: 297 LREKLDELERVFAQYQPQIAGILGHLQNFIAAKQAERLVFVENETLKRQLTELQDDYRIA 356 Query: 301 LKSRSWTFWAMLVTMVMALLSAIGIALVQLQSSRNNAAAAEARRQQAELQRQLAQQEEEK 360 LKSRSWTFWAMLVTMVMALLSAIGIALVQLQSSRNNAAAAEARRQQAELQRQLAQQEEEK Sbjct: 357 LKSRSWTFWAMLVTMVMALLSAIGIALVQLQSSRNNAAAAEARRQQAELQRQLAQQEEEK 416 Query: 361 AMRVNHLTQTAILRLMTELQKVADGDLTVRTTVSDDVTGAIADSVNHTVEALRALVEQVT 420 AMRVNHLTQTAILRLMTELQKVADGDLTVRTTVSDDVTGAIADSVNHTVEALRALVEQVT Sbjct: 417 AMRVNHLTQTAILRLMTELQKVADGDLTVRTTVSDDVTGAIADSVNHTVEALRALVEQVT 476 Query: 421 QMASKVSQSSEQVRGTTTELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVAR 480 QMASKVSQSSEQVRGTTTELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVAR Sbjct: 477 QMASKVSQSSEQVRGTTTELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVAR 536 Query: 481 HAVLAAERGERAVENAIKAMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNV 540 HAVLAAERGERAVENAIKAMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNV Sbjct: 537 HAVLAAERGERAVENAIKAMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNV 596 Query: 541 LALNAAIQAASAGEAGRGFSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDK 600 LALNAAIQAASAGEAGRGFSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDK Sbjct: 597 LALNAAIQAASAGEAGRGFSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDK 656 Query: 601 STTGVTEGAVLSDAAGSALADIRSVSEKLAELIQGMAGSTREQAVSANGVAQQIQGILTE 660 STTGVTEGAVLSDAAGSALADIRSVSEKLAELIQGMAGSTREQAVSANGVAQQIQGILTE Sbjct: 657 STTGVTEGAVLSDAAGSALADIRSVSEKLAELIQGMAGSTREQAVSANGVAQQIQGILTE 716 Query: 661 NDIIEQSREQVSALYEELWGAARQLENSVSRFRVTVS 697 NDIIEQSREQVSALYEELW AARQLENSVSRFRVTVS Sbjct: 717 NDIIEQSREQVSALYEELWAAARQLENSVSRFRVTVS 753 >gi|300310620|ref|YP_003774712.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 573 Score = 114 bits (284), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 90/303 (29%), Positives = 157/303 (51%), Gaps = 35/303 (11%) Query: 385 GDLTVRTTVSDDVTGAIADSVNHTVEALRALVEQVTQMASKVSQSSEQVRGTTTELGAAA 444 GDL+ +S G++ +++ +LR +V+ V + ++ S Q+ +L A Sbjct: 239 GDLSTHIDISHAAQGSVVSAMHMMQGSLRKVVQTVRTSSESIATGSRQIATGNLDLSART 298 Query: 445 RAQARRIQRIAQAMLEMSMQITGISVSADESADVARHAVLAAERGERAVENAIKAMNGIR 504 QA ++ A +M E++ + + +A + ++A A A+ G V + M+ I Sbjct: 299 EQQASALEETASSMEELTSTVKHNASNAQNAKEMAATASTVAQEGGEVVAQVVDTMSAIN 358 Query: 505 EQIQETSGRITRLGESSQMIGEITELISDITERTNVLALNAAIQAASAGEAGRGFSVVAE 564 E SS+ + +I +I I +TN+LALNAA++AA AGE GRGF+VVA Sbjct: 359 E--------------SSRKMADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAT 404 Query: 565 EVQHLAERSSAATREIGALVRMIQADTRDVVVAMDKSTTGVTEGAVLSDAAGSALADIRS 624 EV++LA+RS+AA +EI AL+ + S VT GA L+D AG+ ++ I Sbjct: 405 EVRNLAQRSAAAAKEIKALI--------------EDSVDKVTVGARLADQAGTTMSGIVE 450 Query: 625 VSEKLAELIQGMAGSTREQAVSANGVAQQIQGILTENDIIEQSREQVSALYEELWGAARQ 684 + K+ +I ++ ++ EQ G+ Q Q I+ +D+ +Q+ ++L EE A+R Sbjct: 451 NAGKVTSIIGEISAASTEQTA---GIEQINQAIMQMDDVTQQN----ASLVEEAAAASRA 503 Query: 685 LEN 687 L++ Sbjct: 504 LQD 506 >gi|300310634|ref|YP_003774726.1| methyl-accepting chemotaxis domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 568 Score = 111 bits (277), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 99/337 (29%), Positives = 170/337 (50%), Gaps = 37/337 (10%) Query: 362 MRVNHLTQTAILRLMTELQKVADGDLTVRTTVS-DDVTGAIADSVNHTVEALRALVEQVT 420 M V H + + + ++VA GDLT + + TG + +++ L+ +V V Sbjct: 253 MLVYHAIRNPLAHAASVARRVAHGDLTAQIDPAIKGETGELLEALTIMQSGLQTIVLDVR 312 Query: 421 QMASKVSQSSEQVRGTTTELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVAR 480 A ++ S+ ++ T +L + QA ++ A +M E++ + + +A ++++A Sbjct: 313 NGAQYINTSANEIASGTLDLSSRTEQQASSLEETASSMEELTSTVKQNAGNARLASELAN 372 Query: 481 HAVLAAERGERAVENAIKAMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNV 540 A A RG V + M I ESS+ I +I +I I +TN+ Sbjct: 373 TASGVAVRGGEVVARVVDTMGAI--------------NESSKRIVDIIAVIDGIAFQTNI 418 Query: 541 LALNAAIQAASAGEAGRGFSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDK 600 LALNAA++AA AGE GRGF+VVA EV+ LA+RS+AA +EI L+ D Sbjct: 419 LALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKTLI--------------DN 464 Query: 601 STTGVTEGAVLSDAAGSALADIRSVSEKLAELIQGMAGSTREQAVSANGVAQQIQGILTE 660 S + V G+ L + AGS + ++ +++ E+I G++ +++EQ A+G+ +QIQ + + Sbjct: 465 SVSKVEAGSTLVNEAGSTMREVVESVQRVTEIITGISNASQEQ---ASGI-EQIQQAINQ 520 Query: 661 NDIIEQSR----EQVSALYEELWGAARQLENSVSRFR 693 D + Q EQ SA E + A +L + V F+ Sbjct: 521 MDQVTQQNAALVEQASAASESMQMQAHKLTDLVGAFK 557 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 110 bits (276), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 86/272 (31%), Positives = 144/272 (52%), Gaps = 29/272 (10%) Query: 380 QKVADGDLTVRT-TVSDDVTGAIADSVNHTVEALRALVEQVTQMASKVSQSSEQVRGTTT 438 Q+VA GDLT+R D TGA+ +++ E+LR +V +V Q ++ +S ++ Sbjct: 227 QRVARGDLTLRIRPAGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNL 286 Query: 439 ELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVARHAVLAAERGERAVENAIK 498 +L QA ++ A AM E++ +T + +A ++ +A A A +G V ++ Sbjct: 287 DLSNRTEQQAGSLEETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQ 346 Query: 499 AMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNVLALNAAIQAASAGEAGRG 558 M GI E SS+ I +I +I I +TN+LALNAA++AA AGE GRG Sbjct: 347 TMEGITE--------------SSRRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRG 392 Query: 559 FSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDKSTTGVTEGAVLSDAAGSA 618 F+VVA EV+ LA+RS+ A +EI L+ D S V+ G+ L + AGS Sbjct: 393 FAVVASEVRSLAQRSAGAAKEIKELI--------------DDSVQKVSAGSALVERAGST 438 Query: 619 LADIRSVSEKLAELIQGMAGSTREQAVSANGV 650 + ++ + ++ +++ ++ +T+EQ+ N V Sbjct: 439 MTEVVASVRRVTDVVAEISAATQEQSGGINEV 470 >gi|300313877|ref|YP_003777969.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 539 Score = 108 bits (270), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 94/267 (35%), Positives = 144/267 (53%), Gaps = 31/267 (11%) Query: 380 QKVADGDLTVRTTV-SDDVTGAIADSVNHTVEALRALVEQVTQMASKVSQSSEQVRGTTT 438 ++VAD DLT + V S D TG + ++NH ++L +V +V + S + +S ++ Sbjct: 245 RQVADNDLTGQVRVHSADETGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNA 304 Query: 439 ELGAAARAQARRIQRIAQAMLEMSMQITGISVSADESADVARHAV-LAAERGERAVENAI 497 +L A +QA ++ A AM E++ + ++AD A A L A AV+ Sbjct: 305 DLSARTESQASSLEETASAMEELTSTVR-------QNADHAHQANELVATSSRLAVQGG- 356 Query: 498 KAMNGIREQIQETSGRITRLGESSQMIGEITELISDITERTNVLALNAAIQAASAGEAGR 557 + +Q+ +T G I R G S I +IT +I I +TN+LALNAA++AA AGE GR Sbjct: 357 ----AVVDQVVQTMGEI-RTG--SARIADITGVIDGIAFQTNILALNAAVEAARAGEQGR 409 Query: 558 GFSVVAEEVQHLAERSSAATREIGALVRMIQADTRDVVVAMDKSTTGVTEGAVLSDAAGS 617 GF+VVA EV+ LA+RS+AA REI AL+ + S V GA L D AG Sbjct: 410 GFAVVATEVRSLAQRSAAAAREIKALI--------------EGSVQQVATGAELVDQAGQ 455 Query: 618 ALADIRSVSEKLAELIQGMAGSTREQA 644 + +I ++A L+ +A ++REQ+ Sbjct: 456 TMHEIVDSVARVATLMSEIAVASREQS 482 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,636,421 Number of extensions: 59478 Number of successful extensions: 500 Number of sequences better than 1.0e-08: 68 Number of HSP's gapped: 390 Number of HSP's successfully gapped: 69 Length of query: 697 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 605 Effective length of database: 1,184,250 Effective search space: 716471250 Effective search space used: 716471250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)