BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0026.0009.p (718 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 1337 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 193 3e-50 gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 185 9e-48 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 176 9e-45 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 159 6e-40 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 1337 bits (3459), Expect = 0.0, Method: Compositional matrix adjust. Identities = 715/722 (99%), Positives = 715/722 (99%), Gaps = 4/722 (0%) Query: 1 LQETLQPLLKILRQQGSSWRARVDSGLVCPAPPLYLRTLTAVRDCTRSAGAADLEACASA 60 LQETLQPLLKILRQQGSSWRARVDSGLVCPAPPLYLRTLTAVRDCTRSAGAADLEACASA Sbjct: 23 LQETLQPLLKILRQQGSSWRARVDSGLVCPAPPLYLRTLTAVRDCTRSAGAADLEACASA 82 Query: 61 ILRVLECAQPPGQGLDIPALDIIERAVAALAQRGSNRVALEELAALSQPPRMRSSPAARD 120 ILRVLECAQPPGQGLDIPALDIIERAVAALAQRGSNRVALEELAALSQPPRMRSSPAARD Sbjct: 83 ILRVLECAQPPGQGLDIPALDIIERAVAALAQRGSNRVALEELAALSQPPRMRSSPAARD 142 Query: 121 TAPAPWLAPREQQWEGLQDELDQALLPVFLEEAQELMSSIGEGLEALRQGVADAELAGLA 180 TAPAPWLAPREQQWEGLQDELDQALLPVFLEEAQELMSSIGEGLEALRQGVADAELAGLA Sbjct: 143 TAPAPWLAPREQQWEGLQDELDQALLPVFLEEAQELMSSIGEGLEALRQGVADAELAGLA 202 Query: 181 RPLHTLKGSARMVGARRLSHCLHQMETALRGVQTGIEGVHDRSSATLAALETLLGLHDLA 240 RPLHTLKGSARMVGARRLSHCLHQMETALRGVQTGIEGVHDRSSATLAALETLLGLHDLA Sbjct: 203 RPLHTLKGSARMVGARRLSHCLHQMETALRGVQTGIEGVHDRSSATLAALETLLGLHDLA 262 Query: 241 LDHFAALTQAALLLSADGGARAPVLPAPLIRIRTTVLDKLLNQVGEVSIARSRLDNEVGS 300 LDHFAALTQAALLLSADGGARAPVLPAPLIRIRTTVLDKLLNQVGEVSIARSRLDNEVGS Sbjct: 263 LDHFAALTQAALLLSADGGARAPVLPAPLIRIRTTVLDKLLNQVGEVSIARSRLDNEVGS 322 Query: 301 LQSESKALGEQLMRLSGELRQ----SGAQAQDGGDAVAAMQALAHSLAARLEQASLHQRR 356 LQSESKALGEQLMRLSGELRQ SG QAQDGGDA AAMQALAHSLAARLEQASLHQRR Sbjct: 323 LQSESKALGEQLMRLSGELRQWLRQSGTQAQDGGDAAAAMQALAHSLAARLEQASLHQRR 382 Query: 357 LQEGVSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDL 416 LQEGVSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDL Sbjct: 383 LQEGVSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDL 442 Query: 417 SNCRLQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAV 476 SNCRLQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAV Sbjct: 443 SNCRLQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAV 502 Query: 477 IRVSDDGRGLDLEAIRQKALELGAAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGM 536 IRVSDDGRGLDLEAIRQKALELGAAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGM Sbjct: 503 IRVSDDGRGLDLEAIRQKALELGAAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGM 562 Query: 537 DVVRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIV 596 DVVRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIV Sbjct: 563 DVVRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIV 622 Query: 597 QLRGPQARQALAERSLEHQGSRLPLHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAV 656 QLRGPQARQALAERSLEHQGSRL LHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAV Sbjct: 623 QLRGPQARQALAERSLEHQGSRLALHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAV 682 Query: 657 LADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIINPLLLAGAGRTDGMAVAPG 716 LADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIINPLLLAGAGRTDGMAVAPG Sbjct: 683 LADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIINPLLLAGAGRTDGMAVAPG 742 Query: 717 VQ 718 VQ Sbjct: 743 VQ 744 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 193 bits (491), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 111/332 (33%), Positives = 188/332 (56%), Gaps = 3/332 (0%) Query: 368 LRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAI 427 + Q + +TR+LQ+ +M RM+ + + R +VR +A + GK + ++D+ + Sbjct: 350 VSQLSRNTRDLQESVMSIRMMPMDYVFSRFPRMVRDLAHKLGKQVEFVTHGAATELDKGL 409 Query: 428 LERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLD 487 +ER++ PL HL+RN+ HGIE + R A GK G L++ A H+G +I V+DDG GL+ Sbjct: 410 IERIVDPLTHLVRNSIDHGIELPDARRASGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLN 469 Query: 488 LEAIRQKALELGAAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALG 547 E I KA + G S A + LIF+PG ST+ QV+ ++GRG+GMDVV+ + A+G Sbjct: 470 REKILAKAKQQGMPVTDNLSDAEVWQLIFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMG 529 Query: 548 GWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIP-SMLVDSIVQLRGPQARQA 606 G + + S G G ++ LPL+LA+ +R G++ + +P +V+S+ L + Sbjct: 530 GAVDIRSSKGFGTTISISLPLTLAILDGMSIRIGEEVYILPLGYVVESLQPLAQDIKEIS 589 Query: 607 LAERSLEHQGSRLPLHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAVLADHVSGNRE 666 + L +G LPL P + + + EP+ V++++ D A+ D + G ++ Sbjct: 590 GQGQVLRVRGEYLPLIPLYQIFN--IEPSFTDPSQGLVVILESDGKKAALFVDDLIGQQQ 647 Query: 667 VVVKPVGPQLATLAGVVGATVLGDGQIVLIIN 698 +VVK + +AG+ GAT+LGDG + LI++ Sbjct: 648 IVVKNLESNYRKVAGISGATILGDGGVALIVD 679 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 185 bits (470), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 131/390 (33%), Positives = 201/390 (51%), Gaps = 35/390 (8%) Query: 310 EQLMRLSGELRQSGAQAQDGGDAVAAMQALAHSLAARLEQASLHQRRLQEGVSSTREGLR 369 +QL+ L GEL +GA A+ L AR + S + Q + + E +R Sbjct: 342 DQLIDLVGELVIAGASAR---------------LIARRRKDSQFEEATQ-AIDTLMEQIR 385 Query: 370 QQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILE 429 A + RMV+ N + R +VR +A + GK + L ++ ++D++++E Sbjct: 386 DAA----------LTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVE 435 Query: 430 RLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLE 489 RL PL H++RNA HGIE A +RLA GKPA L + A HE VI V DDGRG+D E Sbjct: 436 RLADPLMHIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDRE 495 Query: 490 AIRQKALELG-AAGLAEASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGG 548 AI KA+ G A+ AE S A + +F+PG ST+ QV+ L+GRG+GMDVVR + AL G Sbjct: 496 AILAKAVAQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHG 555 Query: 549 WMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALA 608 + + + G+G L LPL+LA+ + G F IP +V + + Q + Sbjct: 556 EVSIDTGRGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQPGSHI- 614 Query: 609 ERSLEHQGSRLPLHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAVLADHVSGNREVV 668 + + LP P L D G S +++++ +L D + G + V Sbjct: 615 ---VNVRDEPLPFVPLRELFDLPARERGRNS----LVIVQYGPLRAGLLVDGLLGECQAV 667 Query: 669 VKPVGPQLATLAGVVGATVLGDGQIVLIIN 698 +KP+G + G+ G+T+LGDG++ LI++ Sbjct: 668 IKPLGRLFGKVKGLSGSTILGDGRVALILD 697 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 176 bits (445), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 130/380 (34%), Positives = 200/380 (52%), Gaps = 24/380 (6%) Query: 330 GDAVAAMQALAHSLAARLEQASLHQRRLQEGVSS-TREGLRQQAAHTR---ELQQELMYA 385 G+ V + AL + LA R E Q GV RE Q A R E+Q +M Sbjct: 466 GEMVVSKNALPY-LAQRAED--------QFGVRELAREIKAQYAVINRIAEEMQDAIMQV 516 Query: 386 RMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILERLMGPLEHLLRNAAVH 445 RM+ + + R LVR ++ + GK + L L + D+ I+E L PL H++RN+ H Sbjct: 517 RMMPVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDH 576 Query: 446 GIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEAIRQKALELGAAGLAE 505 G+E EQR A GKPA+G LT+ A HEG++ VI + DDG+G+D I++KA E G + E Sbjct: 577 GLEGPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGL--IDE 634 Query: 506 ASQARLAD-----LIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGWMRVHSQPGQGV 560 A R++D L+F G ST+ VS L+GRG+GMDVVR+ V + G + +HS G+G Sbjct: 635 AQLERISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGT 694 Query: 561 QFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALAERSLEH--QGSR 618 + + LPLS+AV V ++ Q F +P V V R P++ A S + +G Sbjct: 695 RLQISLPLSMAVTKVMIIESDGQLFGVPMEHVMETV--RVPRSSLHFANSSAKMVLRGHI 752 Query: 619 LPLHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMAVLADHVSGNREVVVKPVGPQLAT 678 L L + +L A E VL+++ + ++ D +V+ KP+ L Sbjct: 753 LGLKGINQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGG 812 Query: 679 LAGVVGATVLGDGQIVLIIN 698 L+ G+ ++GDG +++++N Sbjct: 813 LSLYSGSALMGDGSVLMVLN 832 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 159 bits (403), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 112/360 (31%), Positives = 191/360 (53%), Gaps = 22/360 (6%) Query: 361 VSSTREGLRQQAAHTRELQQELMYARMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCR 420 +S+ E L + + ++ ELQ+ + RM + + LVR++A + GK + L ++ Sbjct: 295 MSTLAETLGRASRYSSELQRATLSTRMQPVGRLFQKFPRLVRELAKDLGKDVDLVITGAE 354 Query: 421 LQIDRAILERLMGPLEHLLRNAAVHGIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVS 480 ++DR +++ L PL H+LRN+ HGIE R+A GKP +++ A G+ +I V Sbjct: 355 TEVDRVVVDSLYDPLVHMLRNSLDHGIEV--DRVAAGKPTKATISLNAWQAGSSVMIEVF 412 Query: 481 DDGRGLDLEAIRQKALELGAAGLAEASQARLAD----LIFQPGLSTSPQVSALAGRGIGM 536 DDG+G+D + +R KAL A GL QA AD L+F PG ST S+++GRG+GM Sbjct: 413 DDGKGMDPDMLRSKAL---AKGLINEHQALTADEALQLVFLPGFSTKEVASSVSGRGVGM 469 Query: 537 DVVRSEVAALGGWMRVHSQPGQGVQFTLYLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIV 596 DVV++ V G +R+ S G+G +F++ LP+ L++ L+R A+P +V+ +V Sbjct: 470 DVVKTAVEKHRGTIRIESTIGKGTKFSIRLPIELSIIPTMLVRCAGAPLALPMAVVERVV 529 Query: 597 QLRGPQARQAL-AERSLEHQGSRLPLHPWHCLLDEAPEPAGEGSDSAYVLLIKRDDGLMA 655 +L P+ + L QG LP++ +L P G++ +++ ++ Sbjct: 530 EL--PENFDEVGGAPVLRDQGRPLPVNSLAGVLGYEP-----GNERVGIVMAVPHPYILG 582 Query: 656 VLADHVSGNREVVVKPVGPQLATLAGVVGATVLGDGQIVLIIN-PLLLAGAGRTDGMAVA 714 V + V G ++V+KP+ AG+ G +G++VL++ LL G + AVA Sbjct: 583 V--ESVEGTADLVIKPL--TAIHTAGITGTARSAEGELVLVVGLSFLLDGCRDMERTAVA 638 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,117,743 Number of extensions: 89619 Number of successful extensions: 488 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 480 Number of HSP's successfully gapped: 7 Length of query: 718 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 626 Effective length of database: 1,184,250 Effective search space: 741340500 Effective search space used: 741340500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)