BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0025.0007.p
         (426 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309431|ref|YP_003773523.1| ammonium transporter transmembr...   838   0.0  
gi|300309914|ref|YP_003774006.1| ammonium transporter transmembr...   117   2e-27

>gi|300309431|ref|YP_003773523.1| ammonium transporter transmembrane
           protein [Herbaspirillum seropedicae SmR1]
          Length = 510

 Score =  838 bits (2166), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 426/426 (100%), Positives = 426/426 (100%)

Query: 1   MLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFMIFSLIYILWFVYGYSIAFTEGN 60
           MLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFMIFSLIYILWFVYGYSIAFTEGN
Sbjct: 85  MLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFMIFSLIYILWFVYGYSIAFTEGN 144

Query: 61  AFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQGAFAAITVALIIGAFAERVKFS 120
           AFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQGAFAAITVALIIGAFAERVKFS
Sbjct: 145 AFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQGAFAAITVALIIGAFAERVKFS 204

Query: 121 AVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEKATAISGWLFQKGALDYAGGTVV 180
           AVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEKATAISGWLFQKGALDYAGGTVV
Sbjct: 205 AVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEKATAISGWLFQKGALDYAGGTVV 264

Query: 181 HINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTMVGASLLWFGWFGFNAGSGLEANGTA 240
           HINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTMVGASLLWFGWFGFNAGSGLEANGTA
Sbjct: 265 HINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTMVGASLLWFGWFGFNAGSGLEANGTA 324

Query: 241 ALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAITPAAGFVGVIGAVVM 300
           ALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAITPAAGFVGVIGAVVM
Sbjct: 325 ALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAITPAAGFVGVIGAVVM 384

Query: 301 GLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGAILTGVFAAPSLGGTGIYDYVA 360
           GLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGAILTGVFAAPSLGGTGIYDYVA
Sbjct: 385 GLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGAILTGVFAAPSLGGTGIYDYVA 444

Query: 361 NAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIVDVVIGLRVTEEEEREGLDISSH 420
           NAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIVDVVIGLRVTEEEEREGLDISSH
Sbjct: 445 NAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIVDVVIGLRVTEEEEREGLDISSH 504

Query: 421 GESAYH 426
           GESAYH
Sbjct: 505 GESAYH 510


>gi|300309914|ref|YP_003774006.1| ammonium transporter transmembrane
           protein [Herbaspirillum seropedicae SmR1]
          Length = 401

 Score =  117 bits (292), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 116/427 (27%), Positives = 190/427 (44%), Gaps = 59/427 (13%)

Query: 6   AFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFMIFSLIYILWFVYGYSIAFTEGNAFFGG 65
           A +IL    G      G VR KN ++ L++    F++  I++F  GY +A+    +FF G
Sbjct: 18  AIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYFFIGYYVAYRV--SFFAG 75

Query: 66  FDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQGAFAAITVALIIGAFAERVKFSAVILF 125
            + L        +    F       ELV   F   FAA   A+I G  AER KF+  ++ 
Sbjct: 76  AETL--------AQRSGF-------ELVKFFFLLTFAAAIPAIISGGIAERSKFNPQLVA 120

Query: 126 SILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEKATAISGWL---FQKGALDYAGGTVVHI 182
           + +     Y               F +  A  +   +  WL   F     D+AG  VVH 
Sbjct: 121 TAVLVGLVY--------------PFFEGIAWNQHLGVQAWLAATFDAEFHDFAGSIVVHA 166

Query: 183 NAAIAGLVGSYVIGKRIG-FGKE----AFKPHSLTLTMVGASLLWFGWFGFNAGSGLEAN 237
                 L    ++G R G + KE    A  P ++    +GA +L  GWFGFN  S    +
Sbjct: 167 VGGWIALPAVLLLGARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGWFGFNVMSAQTLD 226

Query: 238 GTAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAITPAAGFVGVIGA 297
               L  +N+++A A   L       +G+  P    A +G +AGLVA+   +  +  +GA
Sbjct: 227 KMNGLVAMNSLMAMAGGTL---VALLMGRNDPGF--AYNGPLAGLVAVCAGSDLMHPLGA 281

Query: 298 VVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILGAILTGVFAAPSLGGTGIYD 357
           +V G IAG + +W  +  +     DD L V+ +HG+ G+ G +  G+F   +LGG G   
Sbjct: 282 LVTGGIAGAIFVWMFTRTQNKWKIDDVLGVWPLHGLCGLWGGLAAGIFGLQALGGRGGVS 341

Query: 358 YVANAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIVDVVIGLRVTEEEEREGLDI 417
           +++               Q  G +  +V++ +  +I Y ++   +G+R+  E+E EG D+
Sbjct: 342 FLS---------------QLIGSVMGIVIAALGGWIVYGLLKKAVGIRLDPEQEFEGADL 386

Query: 418 SSHGESA 424
           + H  S+
Sbjct: 387 AIHKISS 393


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.142    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,344,183
Number of extensions: 58313
Number of successful extensions: 275
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 269
Number of HSP's successfully gapped: 2
Length of query: 426
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 338
Effective length of database: 1,203,190
Effective search space: 406678220
Effective search space used: 406678220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 132 (55.5 bits)