BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0025.0005.p (112 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309430|ref|YP_003773522.1| nitrogen regulatory PII-like pr... 218 1e-58 gi|300311220|ref|YP_003775312.1| nitrogen regulatory P-II protei... 162 8e-42 >gi|300309430|ref|YP_003773522.1| nitrogen regulatory PII-like protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 218 bits (554), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 112/112 (100%), Positives = 112/112 (100%) Query: 1 MKLITAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKTK 60 MKLITAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKTK Sbjct: 1 MKLITAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKTK 60 Query: 61 IEAAVDDAIVERALEAIETAARTGKIGDGKIFVQDLLDVIRIRTGETGKDAL 112 IEAAVDDAIVERALEAIETAARTGKIGDGKIFVQDLLDVIRIRTGETGKDAL Sbjct: 61 IEAAVDDAIVERALEAIETAARTGKIGDGKIFVQDLLDVIRIRTGETGKDAL 112 >gi|300311220|ref|YP_003775312.1| nitrogen regulatory P-II protein [Herbaspirillum seropedicae SmR1] Length = 112 Score = 162 bits (409), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 88/112 (78%), Positives = 100/112 (89%) Query: 1 MKLITAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKTK 60 MK +TAIIKPFKLDEVRE+L+ +GV G+TVTEVKGFGRQKGHTELYRGAEYVVDFLPK K Sbjct: 1 MKQVTAIIKPFKLDEVRESLAEVGVTGLTVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK 60 Query: 61 IEAAVDDAIVERALEAIETAARTGKIGDGKIFVQDLLDVIRIRTGETGKDAL 112 IE VDD +VE+A++AI AARTGKIGDGKIFVQ++ VIRIRTGETG DA+ Sbjct: 61 IEVVVDDKVVEQAVDAIIKAARTGKIGDGKIFVQEVEQVIRIRTGETGPDAV 112 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 330,587 Number of extensions: 12365 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 2 Length of query: 112 Length of database: 1,619,870 Length adjustment: 73 Effective length of query: 39 Effective length of database: 1,274,215 Effective search space: 49694385 Effective search space used: 49694385 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)