BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0526.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314076|ref|YP_003778168.1| LysR family transcription regul... 585 e-168 gi|300310159|ref|YP_003774251.1| LysR family transcription regul... 111 8e-26 gi|300309386|ref|YP_003773478.1| LysR family transcription regul... 89 4e-19 gi|300309393|ref|YP_003773485.1| LysR family transcription regul... 87 2e-18 gi|300313217|ref|YP_003777309.1| LysR family transcription regul... 86 5e-18 >gi|300314076|ref|YP_003778168.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 294 Score = 585 bits (1507), Expect = e-168, Method: Compositional matrix adjust. Identities = 291/294 (98%), Positives = 292/294 (99%) Query: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE Sbjct: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 Query: 61 GNKLLPLAERVLADVQELRRHVADFRSSTGELSGSLAIGTILDPEFIRLGGFLKLLVERH 120 GNKLLPLAERVLADVQELRRH ADFRSSTGELSGSLAIGTILDPEFIRLGGFLKLLVERH Sbjct: 61 GNKLLPLAERVLADVQELRRHAADFRSSTGELSGSLAIGTILDPEFIRLGGFLKLLVERH 120 Query: 121 PQLSTQLAHHMSGSVLNEIRSGKLDVGFFLGAPGQGFQSMELTSFTYKVIAPAGWKNRVS 180 PQLSTQLAHHMSGSVLNEIR+GKLDVGFFLGAPGQGFQSMELTSFTYKVIAPAGWKNRVS Sbjct: 121 PQLSTQLAHHMSGSVLNEIRAGKLDVGFFLGAPGQGFQSMELTSFTYKVIAPAGWKNRVS 180 Query: 181 GKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQVKPHTVALVDQESSMLDLVKSGVGLSL 240 GKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQVKPHTVALVDQESSMLDLVKSGVGLSL Sbjct: 181 GKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQVKPHTVALVDQESSMLDLVKSGVGLSL 240 Query: 241 ARASIALNQAHAHGLVIADAVELSTQLSFICLEKRQHDPVIQAVFRLLQEIWQS 294 ARASIALNQAHAHGLVIADAVELSTQLSFICLEK QHDPVIQAVFRLLQEIWQS Sbjct: 241 ARASIALNQAHAHGLVIADAVELSTQLSFICLEKWQHDPVIQAVFRLLQEIWQS 294 >gi|300310159|ref|YP_003774251.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 309 Score = 111 bits (277), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 95/285 (33%), Positives = 144/285 (50%), Gaps = 22/285 (7%) Query: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 M+ LR F+ V G++ AA +LH+ QPA+S QIK L+E LG LF+RS G LTA Sbjct: 1 MEFRHLRYFLGVVEHGSVAEAARRLHIAQPALSRQIKYLEEELGAALFDRSVRGATLTAA 60 Query: 61 GNKLLPLAERVLADVQELRRHVADFRSSTGELSGSLAIGTILDPEF---IRLGGFLKLLV 117 G +L+ A R+LAD+QE R+ VA R + G L G+L +G + P F R+ G L+ Sbjct: 61 GQQLVIDARRMLADLQETRQRVA--RVAEG-LEGNLRMG--ITPNFGWHPRILGSLRHFH 115 Query: 118 ERHPQLSTQLAHHMSGSVLNEIRSGKLDVGFFL--GAPGQGF---QSMELTSFTYKVIAP 172 + PQ++ L +S L IRSG LD GF P F + E+ F ++ Sbjct: 116 QAEPQIAVSLEPGLSARQLERIRSGDLDAGFLAWRYPPNDQFLAHEFGEIQVFECRLKLA 175 Query: 173 AGWKNRVSGKGWKELSRL---PWIWTPPESA--HHRLLSRIFAQHQVKPHTVALVDQESS 227 ++++ + + L+ L P IW P E A + + L+ + P L S Sbjct: 176 LPRDSQLARQVPRHLAALHDEPAIWFPAEVAPGYDQFLNYQCQMVNLVPRKAQLASDVLS 235 Query: 228 MLDLVKSGVGLSLARASIALNQAHAHGLVIADAVELSTQ--LSFI 270 +L LV +GVG ++ A G+V+ D EL+ + +SF+ Sbjct: 236 ILGLVAAGVGYAIVSDVSAYTC--PEGVVLVDHPELTLRHPVSFV 278 >gi|300309386|ref|YP_003773478.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 301 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 70/246 (28%), Positives = 115/246 (46%), Gaps = 13/246 (5%) Query: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 MD+ LR F + +RAAD LH+TQ VS ++ L+E + L R + LT Sbjct: 1 MDIRALRYFTETVRHSSFSRAADALHVTQSTVSKMVRQLEEEVDAPLLIRDGRRLQLTDT 60 Query: 61 GNKLLPLAERVLADVQELRRHVADFRSSTGELSGSLAIGTILDPEFIRL--GGFLKLLVE 118 G + +++LA +Q L A+ R + SG L IG P I + LK E Sbjct: 61 GRVVYEQGQQILAAMQRLH---AEVRDTKALRSGRLEIGI---PPMINILCTPVLKAFRE 114 Query: 119 RHPQLSTQLAHHMSGSVLNEIRSGKLDVGFFL--GAPGQGFQSMELTSFTYKVIAPAG-- 174 RHP ++ L + + +G+L++G + P G +++ + S+ +A AG Sbjct: 115 RHPDIAVVLQEDTGPQIEQRVANGELEIGMTVLPADPELGLETLPVASYPMWAVAQAGSF 174 Query: 175 WKNRVSGKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQVKPHTVALVDQESSMLDLVKS 234 KNR S + +L +P + E R L +F +H +KP VA Q ++ + + Sbjct: 175 QKNRASLRC-AQLDGMPLVLLNNEFGLTRSLRGVFREHGIKPEIVAQSAQWDWLVAMALA 233 Query: 235 GVGLSL 240 G+G +L Sbjct: 234 GMGTAL 239 >gi|300309393|ref|YP_003773485.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 75/252 (29%), Positives = 121/252 (48%), Gaps = 11/252 (4%) Query: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 M+L LR F++VA + + TRAA+K+ + QP +S+QIK L+E +G LF RS+ G+ L+ Sbjct: 1 MELRHLRYFLAVAEQRSFTRAAEKVGIGQPPLSMQIKALEEEVGGALFLRSSQGVELSEV 60 Query: 61 GNKLLPLAERVLADVQELRRHVADFRS-STGELSGSLAIGTILDPEFIRLGGFLKLLVER 119 G LLP A+R + ++ R S TG L + P + L+ ER Sbjct: 61 GRVLLPHAQRAVDQAEQALRAARQAASGETGLLRMGFTSSAVFHP---LVSHALQQFRER 117 Query: 120 HPQLSTQLAHHMSGSVLNEIRSGKLDVGF--FLGAPGQGFQSMELTSFTYKVIAPAGWK- 176 H +L L+ ++ +L +I + +LD F + P +EL + KV P Sbjct: 118 HRKLEISLSEGITEQLLADIAASQLDAAFVRIVTTPSAALGVVELPAEKLKVALPRSHPL 177 Query: 177 NRVSGKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQV---KPHTVALVDQESSMLDLVK 233 R ++L+ +I P L IF+ Q P + L Q +S ++LV Sbjct: 178 ARRKSLRLQDLASENFIMV-PRGKGSALYDSIFSACQAAGFDPQVIQLAPQLTSAINLVA 236 Query: 234 SGVGLSLARASI 245 SG+G+S+ +I Sbjct: 237 SGLGISIVPEAI 248 >gi|300313217|ref|YP_003777309.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 296 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 79/259 (30%), Positives = 120/259 (46%), Gaps = 13/259 (5%) Query: 1 MDLTQLRAFISVAHEGNLTRAADKLHLTQPAVSLQIKGLQESLGLQLFNRSAAGMVLTAE 60 M+L +L F +V EG +TRAA++L+ Q V+ +IK L++S+G+ LF R MVLTA Sbjct: 10 MELAELEIFRTVVAEGGVTRAAERLNRVQSNVTTRIKQLEQSMGVPLFVRDRRRMVLTAA 69 Query: 61 GNKLLPLAERVLADVQELRRHVADFRSSTGELSGSLAIGTILDPEFIRLGGFLKLLVERH 120 G LL AER+L QE R+ VA G L IG++ RL G L +R Sbjct: 70 GEALLDYAERILDLAQEARQAVA-----PQSPRGRLRIGSMESAAASRLPGPLAEFHQRW 124 Query: 121 PQLSTQLAHHMSGSVLNEIRSGKLDVGFFLG-APGQGFQSMELTSFTYKVIAPAGWKNRV 179 P++ +L+ + ++ ++R+ KLD G F + L ++ P + RV Sbjct: 125 PEVRLELSTAPTDVLMAQVRACKLDAALVAGPVSDPAFDATPLFREELLLVTPRSHR-RV 183 Query: 180 SGKGWKELSRLPWIWTPPESAHHRLLSRIFAQHQVK---PHTVALVDQESSMLDLVKSGV 236 L L I A+ R FA H P T+ L ++L V +G Sbjct: 184 RAPEEVMLDTL--IVFEQGCAYRRHAEAWFAGHGAGRQLPRTLELASYH-AILACVSAGA 240 Query: 237 GLSLARASIALNQAHAHGL 255 G+++ S+ Q HG Sbjct: 241 GVAVVPRSVIAMQMEPHGF 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 912,701 Number of extensions: 37521 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 55 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 55 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)