BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0525.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 1027 0.0 gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 692 0.0 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 469 e-133 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 223 3e-59 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 203 3e-53 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 1027 bits (2656), Expect = 0.0, Method: Compositional matrix adjust. Identities = 503/503 (100%), Positives = 503/503 (100%) Query: 1 MSLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWA 60 MSLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWA Sbjct: 1 MSLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWA 60 Query: 61 ETAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQL 120 ETAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQL Sbjct: 61 ETAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQL 120 Query: 121 LKTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERD 180 LKTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERD Sbjct: 121 LKTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERD 180 Query: 181 PSPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKAT 240 PSPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKAT Sbjct: 181 PSPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKAT 240 Query: 241 ERAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVA 300 ERAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVA Sbjct: 241 ERAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVA 300 Query: 301 DKLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLK 360 DKLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLK Sbjct: 301 DKLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLK 360 Query: 361 VPGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSL 420 VPGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSL Sbjct: 361 VPGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSL 420 Query: 421 FTADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRY 480 FTADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRY Sbjct: 421 FTADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRY 480 Query: 481 KSIMQRWSATIGKGAEFTMPVAK 503 KSIMQRWSATIGKGAEFTMPVAK Sbjct: 481 KSIMQRWSATIGKGAEFTMPVAK 503 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 692 bits (1786), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/501 (66%), Positives = 393/501 (78%), Gaps = 4/501 (0%) Query: 3 LPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAET 62 + I H+I GQ + SGR DVFNPA G A+VAL + EV+AAV A AA PAW+ T Sbjct: 1 MEKIAHYIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNT 60 Query: 63 APLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLK 122 PL RARVLF+F LI+Q D A+ I EHGK FSDA+GEV RG+EIVEFA GIPQ+LK Sbjct: 61 PPLTRARVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLK 120 Query: 123 TQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPS 182 ++TD I GID W++RQ LGV AGITPFNFP MVP+WM PIAIA GN FVLKPSERDPS Sbjct: 121 GEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPS 180 Query: 183 PSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATER 242 SL++A+L K AGLPDGVFNVVQGDKV VDALL+HP+V+A+SFVGSTPIAEYIY + + + Sbjct: 181 ASLLIADLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAK 240 Query: 243 AGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADK 302 R QALGGAKNH+VVMPDA++ AVDAL+GAAYGSAGERCMAISVA+AVGS D+ Sbjct: 241 GK----RVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDE 296 Query: 303 LVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVP 362 LV AL R + LK+ GM AEMGP+VTAA K ++EG I GV+ GA L+VDGRG +VP Sbjct: 297 LVAALAERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVP 356 Query: 363 GHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFT 422 G E+GFF+GGTL DHVT EM +Y+EEIFGPVLCV+R PD ASAV+LINAHEYGNGV+++T Sbjct: 357 GFEKGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYT 416 Query: 423 ADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKS 482 DG A EF R+I+VGMVG+NVP+PVPMA+ SFGGWKRSLFGD H YG EG+RFYTR K+ Sbjct: 417 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKA 476 Query: 483 IMQRWSATIGKGAEFTMPVAK 503 +MQRW T GAEF P K Sbjct: 477 VMQRWPNTASAGAEFAFPQMK 497 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 469 bits (1207), Expect = e-133, Method: Compositional matrix adjust. Identities = 238/488 (48%), Positives = 327/488 (67%), Gaps = 5/488 (1%) Query: 2 SLPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAE 61 ++P++ ++NG+ +S S +DV NPAT A+V A+ +EV+ AVA A W Sbjct: 5 NIPNVPLYLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRN 64 Query: 62 TAPLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLL 121 T+ +R R++ KF++L+ +N LA+ IT EHGK DA+GEV RGLE+VE A I L Sbjct: 65 TSLAQRMRIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQ 124 Query: 122 KTQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDP 181 + +N+ GG+D + L QPLGV AGIT FNFPVM+P +M PIA+A GN+FVLKPSE+DP Sbjct: 125 LGELAENVAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDP 184 Query: 182 SPSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATE 241 + SL L EL +AGLP GV NVV G + + +HP+++AVSF+GST + +IYR+A+E Sbjct: 185 TSSLFLVELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASE 244 Query: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 AG RAQ + GAKNH +V+PDA DQA++ L+GAA+G+AG+RCMA SV V VG + Sbjct: 245 -AGK---RAQCMMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQTRE 300 Query: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 + E ++ R KA+KV G + A++GPLV+ A K +VE I SGV+ GAKLL+DGR KV Sbjct: 301 WIPE-IVERSKAMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKV 359 Query: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 G E G F+G T+F V EM IY +EIFGP +C+V + A+ INA+ GNG S+F Sbjct: 360 AGSENGNFVGPTVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIF 419 Query: 422 TADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYK 481 T+ G A +F I VG VGINVPIPVP+A+ SF G + S GD G++ + F+T+ K Sbjct: 420 TSSGYAARKFQNEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTK 479 Query: 482 SIMQRWSA 489 ++ RW A Sbjct: 480 TVTARWYA 487 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 223 bits (568), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 165/483 (34%), Positives = 247/483 (51%), Gaps = 17/483 (3%) Query: 5 HIDHF-INGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAETA 63 +DHF I+G+ + V PAT ++AL + +V+ AV AA A PAWA T+ Sbjct: 14 RLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATS 73 Query: 64 PLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAK-GEVTRGLEIVEFATGIPQLLK 122 PL+RA +L + L+ + + A+A+TLE G S A+ V E + A L Sbjct: 74 PLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVARD--NLAD 131 Query: 123 TQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPS 182 F G +R+P+GV A ITP+N+P+ A+A G + VLKPSE P Sbjct: 132 YPFIRPRG---TTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPL 188 Query: 183 PSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLE-HPEVQAVSFVGSTPIAEYIYRKATE 241 +L+ AE+ AG+P GVFN+V G V A L HP+V +S GST + + A Sbjct: 189 SALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAA-- 246 Query: 242 RAGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVAD 301 A T AQ LGG +L V+PDA+L++A+ + AA + G+ C A + + + Sbjct: 247 -AVTVKRVAQELGGKSPNL-VLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALA 304 Query: 302 KLVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKV 361 ++ E + + +KV + + GPL A +V+ I++G+ GA+LL G G + Sbjct: 305 RVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPG-RP 363 Query: 362 PGHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLF 421 G +G++ T+F V T+M I QEEIFGPVL ++ A+ + N YG G + Sbjct: 364 DGLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQ 423 Query: 422 TADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYK 481 D + + RIQ G V +N P P A FGG+K+S G+ YG EG+ Y K Sbjct: 424 GRDKDQVRAVAARIQSGQVHLNYPAWDPQA--PFGGYKQS--GNGREYGVEGMEEYMEVK 479 Query: 482 SIM 484 +I+ Sbjct: 480 AIL 482 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 203 bits (516), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 146/460 (31%), Positives = 225/460 (48%), Gaps = 16/460 (3%) Query: 9 FINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAETAPLKRA 68 F+NGQ ++ G V NPA G V L E A+ AA+AA PAW + +R+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 69 RVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLKTQFTDN 128 +L ++ EL+ N D LA +T E GK ++A+GE+ +E+ + K + D Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEW---FAEEGKRTYGDT 131 Query: 129 IGGGIDNWN---LRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPSPSL 185 I + +++ +GV A ITP+NFP + A A+A G VLKP+E P +L Sbjct: 132 IPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSAL 191 Query: 186 MLAELFKRAGLPDGVFNVVQGDKVAVDA-LLEHPEVQAVSFVGSTPIAEYIYRKATERAG 244 LA L +RAG+P GVF+VV G + + +P V+ +SF GST + + + +++ Sbjct: 192 ALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIK 251 Query: 245 TFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADKLV 304 L LGG +V DA+LD AV+ I + Y +AG+ C+ + V D Sbjct: 252 KLSLE---LGG-NAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFA 307 Query: 305 EALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVPGH 364 L+ VK KV G E+ GPL+ KVE ++ V GA++L+ G+ + GH Sbjct: 308 AKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHAL-GH 366 Query: 365 EEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFTAD 424 F T+ VT M++ +EE FGP+ + R + L N E+G ++ D Sbjct: 367 S---FFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRD 423 Query: 425 GNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFG 464 + ++ GMVGIN + + FGG K+S G Sbjct: 424 IGRIWRVAEGLESGMVGINTGL-ISNEVAPFGGVKQSGLG 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,509,008 Number of extensions: 63515 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 20 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)