BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0520.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 761 0.0 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 559 e-160 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 544 e-156 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 343 2e-95 gi|300313292|ref|YP_003777384.1| branched-chain amino acid ABC t... 79 5e-16 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 761 bits (1966), Expect = 0.0, Method: Compositional matrix adjust. Identities = 378/402 (94%), Positives = 378/402 (94%), Gaps = 24/402 (5%) Query: 20 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 79 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV Sbjct: 25 QISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADV 84 Query: 80 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 139 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV Sbjct: 85 AASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTV 144 Query: 140 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 199 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP Sbjct: 145 HYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHP 204 Query: 200 LGASDFSSFLLQAQSSKAQIIKPVAGLSLYANTTSGLTRGTIVPTGLANAGGDFINSVKA 259 LGASDFSSFLLQAQSSKAQII GLANAGGDFINSVKA Sbjct: 205 LGASDFSSFLLQAQSSKAQII------------------------GLANAGGDFINSVKA 240 Query: 260 ANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKK 319 ANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKK Sbjct: 241 ANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKK 300 Query: 320 MPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDM 379 MPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDM Sbjct: 301 MPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDM 360 Query: 380 YLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKCAAWK 421 YLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKCAAWK Sbjct: 361 YLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKCAAWK 402 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 559 bits (1440), Expect = e-160, Method: Compositional matrix adjust. Identities = 272/420 (64%), Positives = 320/420 (76%), Gaps = 24/420 (5%) Query: 2 ALAITAAAAAFASASASAQISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAI 61 AL+ TA A A+ SAQ +GD IKIGFITD+SG Y+D DGQGG EAI+MAIAD GGAI Sbjct: 9 ALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAGGAI 68 Query: 62 NGKKIELLVADHQNKADVAASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQ 121 NGKK+EL+ ADHQNKAD+AA+KAREWFD+ GVDML+GG NS +LA KVA EKKKV++ Sbjct: 69 NGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFIS 128 Query: 122 IGAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDT 181 +GAG+ R TNEEC+ YT+ YAYDT ALA+GTG+A+ +QGGKSW+FLTADYAFG SLE DT Sbjct: 129 VGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAFGTSLEKDT 188 Query: 182 ANVVKASGGTVVGAVKHPLGASDFSSFLLQAQSSKAQIIKPVAGLSLYANTTSGLTRGTI 241 + VVK++GG VVGA + PL SDFSSFLLQAQSSKAQI+ Sbjct: 189 SEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQIL--------------------- 227 Query: 242 VPTGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWD 301 GLA AGGD IN++KAANEFG+ KT KLAGLL+FIND HSL L T+G+YLTD WYWD Sbjct: 228 ---GLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWD 284 Query: 302 ANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFND 361 N++TRA+A RYF KKMPS QA D SA QYLK VK +G+ D+DKVM LRK + ND Sbjct: 285 LNDQTRAWANRYFMKMKKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRIND 344 Query: 362 MYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKCAAWK 421 + NG +R DGR++HDMYL +VK SESK PWDYYKLVQTIP EQA+ TKAESKCA WK Sbjct: 345 FFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 544 bits (1402), Expect = e-156, Method: Compositional matrix adjust. Identities = 265/419 (63%), Positives = 321/419 (76%), Gaps = 25/419 (5%) Query: 3 LAITAAAAAFASASASAQISGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAIN 62 LA A A S +A AQ+S D ++IGFITD+SG Y+D DG GG EAI+MA+AD GG + Sbjct: 10 LAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVL 69 Query: 63 GKKIELLVADHQNKADVAASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQI 122 GK IE++ ADHQNKAD+AA++AREW D++G+DMLIGG NS TAL+ KV AEKK+VY+ I Sbjct: 70 GKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINI 129 Query: 123 GAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTA 182 GAG+ARLTNEEC+PYTVHY YDTVALAKGT SAV+KQGGKSWFFL ADYAFGHSL +DT+ Sbjct: 130 GAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLANDTS 189 Query: 183 NVVKASGGTVVGAVKHPLGASDFSSFLLQAQSSKAQIIKPVAGLSLYANTTSGLTRGTIV 242 VVKASGGTVVG++KHP +SD SSFLLQAQ+SKAQI+ Sbjct: 190 AVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQIL---------------------- 227 Query: 243 PTGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHSLTLKNTEGLYLTDSWYWDA 302 GLANAG D +NS+KAA EFG+TKT KL GLL+ IND+H+L L N EGL +TDSWYWD Sbjct: 228 --GLANAGEDTVNSIKAAKEFGVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDK 285 Query: 303 NEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIGTDDSDKVMAALRKAKFNDM 362 +E +R FA+R+F KKMPS+ QAA YSA YLK V+ IGTDDS KVMA L+K + +D Sbjct: 286 DEPSRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDF 345 Query: 363 YQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTIPAEQAWTTKAESKCAAWK 421 Y K G +RADGR +HDMYLYQVK+ +ESK PWDY KL++TIP EQA+T+ A+SKC+ K Sbjct: 346 YNK-GSIRADGRNIHDMYLYQVKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 343 bits (879), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 188/417 (45%), Positives = 257/417 (61%), Gaps = 32/417 (7%) Query: 1 MALAITAAAAAFASASASAQISG-----DVIKIGFITDISGVYSDIDGQGGAEAIKMAIA 55 + ++TAAA A + A+ I + +KIG +TD+SG YS + GQG A KMA+ Sbjct: 6 LGRSLTAAAVACTVSLAATAIPAWAQDANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVE 65 Query: 56 D-MGGAINGKKIELLVADHQNKADVAASKAREWFDQQGVDMLIGGTNSGTALASAKVAAE 114 D + G KIE+L AD+QNK DV ++AREW D+ V+ + TNS ALA K+ + Sbjct: 66 DCLKNECKGMKIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKD 125 Query: 115 KKKVYMQIGAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFG 174 K + M G + LT EC+P H+ +DT + A G +A+ K G KSW+F+T DYAFG Sbjct: 126 KGGIAMYSGPATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFG 185 Query: 175 HSLESDTANVVKASGGTVVGAVKHPLGASDFSSFLLQAQSSKAQIIKPVAGLSLYANTTS 234 HSLE D ++VVKA+GGTVVG+V+HPL ASDFSSFLLQAQ+SKAQ+I Sbjct: 186 HSLEKDASDVVKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVI-------------- 231 Query: 235 GLTRGTIVPTGLANAGGDFINSVKAANEFGI-TKTAKLAGLLVFINDVHSLTLKNTEGLY 293 GLA+ G D +N++K A F + K +L LLVF++DVH+L L+ +GL Sbjct: 232 ----------GLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVHALGLEAAQGLV 281 Query: 294 LTDSWYWDANEETRAFAKRYFAVRKKM-PSSLQAADYSAALQYLKVVKEIGTDDSDKVMA 352 TD +YWD +E+TRAF+KR+ + K + P+ +QA YS+ Q+LK T D V Sbjct: 282 YTDGFYWDYDEQTRAFSKRFESQFKNIKPTMVQAGVYSSVYQFLKARAAAKTSDWKVVAQ 341 Query: 353 ALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTIPAEQAW 409 +R+ D +N +R DGRV+HDMYLYQVK +ESK PWDY KL+ IPA+QA+ Sbjct: 342 KMREIPIADAVMRNASIRPDGRVIHDMYLYQVKTPAESKGPWDYLKLLSVIPAQQAF 398 >gi|300313292|ref|YP_003777384.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 391 Score = 79.3 bits (194), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 98/410 (23%), Positives = 177/410 (43%), Gaps = 48/410 (11%) Query: 3 LAITAAAAAFASASASAQISGDVIKIGFITDISGVYSDIDGQ--GGAEAIKMAIADMGGA 60 LA+ AA+ ++ A AQ + IK+G I SG ++D Q GG IK +A G Sbjct: 9 LAVMAASGGLLASLAQAQ---ETIKVGLIAAFSGPFADYGKQMEGG---IKAYMAQHGDQ 62 Query: 61 INGKKIELLVADHQNKA-DVAASKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVY 119 + GKKI++++ D + ++A A+E ++ VD L G + ALA A +A + KK Sbjct: 63 VAGKKIQIIIKDTTGPSPEIAKRLAQELVVREKVDFLAGFGLTPEALAVAPIAEQAKKPM 122 Query: 120 MQIGAGSARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLES 179 + + A ++ +T + S Y ++ ++ + +K G K L ADY G E+ Sbjct: 123 IIMNAATSVITTK--SNYIARFSMTLPQVSGPMATWALKNGIKRVVTLVADYGPGIDAET 180 Query: 180 DTANVVKASGGTVVGAVKHPLGASDFSSFLLQAQSSKAQIIKPVAGLSLYANTTSGLTRG 239 + GG V+ +++ PL +F+ ++ + + +K + + Sbjct: 181 AFKTNLLGGGGQVLESIRVPLRNPEFAPYIQRIKDAKPEAV------------------F 222 Query: 240 TIVPTGLANAGGDFINSVKAANEFGITKTAKLAGLLVF----INDVHSLTLKNTEGLYLT 295 VP AG I +K E G+ + AG+ V + D H + L + Sbjct: 223 IFVP-----AGEQSIAFMKGYRERGLAE----AGIKVIATGDLTDDHVMPAMGESTLGVI 273 Query: 296 DSWYWDA---NEETRAFAKRYFAVRK--KMPSSLQAADYSAALQYLKVVKEI-GTDDSDK 349 ++++ A + E +AF K + A P+ + A Y +V K++ G D D+ Sbjct: 274 TTFHYSAAHDSPENKAFLKSFAAANPGAGRPNFMAVAAYDGMNAIYEVSKKLNGKIDGDR 333 Query: 350 VMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKL 399 MA L+ KF A ++ +Y+ +V+ Y ++ K Sbjct: 334 AMAILKTMKFTSPRGPIAIDPATRDIVQTVYVRKVEKIGNEAYNVEFDKF 383 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,172,319 Number of extensions: 45999 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 10 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)