BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0519.p (306 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 567 e-163 gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC t... 177 1e-45 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 155 5e-39 gi|300312237|ref|YP_003776329.1| amino acid ABC transporter perm... 136 2e-33 gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC t... 130 2e-31 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 567 bits (1461), Expect = e-163, Method: Compositional matrix adjust. Identities = 293/294 (99%), Positives = 294/294 (100%) Query: 13 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG 72 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG Sbjct: 1 MELFGIPLQALLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMG 60 Query: 73 LSYLGLNYWVMLIVAPILVGAFGIIIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI 132 LSYLGLNYWVMLIVAPILVGAFGI+IEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI Sbjct: 61 LSYLGLNYWVMLIVAPILVGAFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSI 120 Query: 133 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGT 192 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGT Sbjct: 121 YGVSGQPYSVPDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGT 180 Query: 193 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG 252 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG Sbjct: 181 ENPKLVEAFGINVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIG 240 Query: 253 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGKEK 306 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGKEK Sbjct: 241 GMGSILGSIITGLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGKEK 294 >gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 177 bits (448), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 106/281 (37%), Positives = 178/281 (63%), Gaps = 1/281 (0%) Query: 24 LSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWVM 83 L Q+ G+ G Y +L++GL+++FGLL +NF+HG ++LGA+L + ++ LGL +W Sbjct: 5 LLQIANGVGIGMLYFLLAVGLSIVFGLLRFVNFSHGAFFLLGAYLCFQ-VTDLGLGFWWS 63 Query: 84 LIVAPILVGAFGIIIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVP 143 L++AP++VG F I+E+ +LR +Y L H + +L T G+ L+I+ S +G G + P Sbjct: 64 LLIAPLVVGLFAWIVERFVLRHVYALPHHFHILFTVGMALVIQEAVISTWGPLGNNVAPP 123 Query: 144 DLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGI 203 DLLQG G V P YR +V+ + ++ W+V+E TRLG+ +RAG+E+ ++V G+ Sbjct: 124 DLLQGVVMWGGFVYPKYRLFVIGLTAVMALLLWWVLEGTRLGSIVRAGSESTEMVSLLGL 183 Query: 204 NVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIIT 263 N+ + +L + G A A AG LAAP+ P MG + F VVVIGG+GS G++I Sbjct: 184 NINRIFSLVFALGAATAGLAGALAAPIRGADPFMGVEALGIAFVVVVIGGLGSFWGALIG 243 Query: 264 GLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGK 304 G+++G+++ + + E + ++++ MA V+++RP GL G+ Sbjct: 244 GILVGIVQSVMSTVWSEGAHLMIYVAMAAVMLLRPNGLLGR 284 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 155 bits (391), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 101/297 (34%), Positives = 170/297 (57%), Gaps = 18/297 (6%) Query: 23 LLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWV 82 + Q+L L G +++ GL +IFG++N +N AHG+ +M+GA++A ++ ++W+ Sbjct: 5 IFEQVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFMIGAYVA-AVVAGASGSFWL 63 Query: 83 MLIVAPILVGAFGIIIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSV 142 L+VA A G ++E + R LY+ DH+ +L TF L+ R I+G P ++ Sbjct: 64 GLLVALPATAALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRMIWGPQPLPLNM 123 Query: 143 PDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFG 202 P G NL + +YR V+ ++V A +F+I +TR+G ++RAG N ++V A G Sbjct: 124 PAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAGANNLEMVTAMG 183 Query: 203 INVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSII 262 IN+ L+ L + FG+ LAA AG++ P++ V MG +++I F VVVIGG+GS+ G+ I Sbjct: 184 INIRLLFRLVFSFGLLLAALAGIMLGPLVAVQAGMGDDVLILAFVVVVIGGIGSVPGAAI 243 Query: 263 TGLVLGLIEGLTRVFYPELSA-----------------TVVFIIMAIVLMVRPAGLF 302 L++G+++ L R P+L A +++++M VL RP+GLF Sbjct: 244 GALIVGVVDTLGRSLLPQLFALFLSADAAASVGPALGSMMIYLVMIAVLFWRPSGLF 300 >gi|300312237|ref|YP_003776329.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 92/286 (32%), Positives = 160/286 (55%), Gaps = 4/286 (1%) Query: 23 LLSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWV 82 L QL+ L GS YA+ +LG ++FG+L VIN +HG ++MLG++ A + + + L WV Sbjct: 2 LEQQLINALSLGSVYALFALGFTLVFGVLGVINLSHGAIFMLGSYAALLLVEQMALPLWV 61 Query: 83 MLIVAPILVGAFGIIIEKTMLRWLYKLD--HLYGLLLTFGITLMIEGIFRSIYGVSGQPY 140 L++A + G G+II+ +L+ L K + HL ++ T G+ +MI I + ++G + + Sbjct: 62 ALLLAMVATGLLGLIIDVLVLKPLRKRNAPHLIPMIATIGVAIMITNISQGLFGAENKRF 121 Query: 141 SVPDLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEA 200 + + + +LG + + + ++V + ++ V+ KT+LG LRA E+PK Sbjct: 122 PQGTIPEESVSLGNLHITAVQVAIIVIAFVLMVVLLGVMRKTQLGRALRAIAESPKAAYL 181 Query: 201 FGINVPLMVTLT-YGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILG 259 GINV + LT + A ++ +SP MG ++ AV+++GGMG I G Sbjct: 182 LGINVEGLFLLTSFAAAALGGAAGVLVGVSFNAISPFMGQPMLHKGIAVIILGGMGDIRG 241 Query: 260 SIITGLVLGLIEGLTRVF-YPELSATVVFIIMAIVLMVRPAGLFGK 304 ++I GL LG E LT + + V F ++ ++L+V+P+G+FGK Sbjct: 242 AMIGGLFLGFAEVLTVAYISSDFRDAVAFGLLFLILLVKPSGMFGK 287 >gi|300313286|ref|YP_003777378.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 91/282 (32%), Positives = 154/282 (54%) Query: 24 LSQLLLGLVNGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLGLNYWVM 83 L L G+ GS ++S+GL+V G++N IN AHG MLG ++ L+ +G+ + Sbjct: 6 LGVLFDGIAYGSLLFLISVGLSVTMGMMNFINLAHGAFAMLGGYVCVTLLNGMGVPFLAT 65 Query: 84 LIVAPILVGAFGIIIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVP 143 L +A I G+++E+++ R LYK HL +L + G+T M +G + QP +P Sbjct: 66 LPLAFIAAAIVGLVLERSLYRRLYKASHLDQVLFSIGLTFMAVAGATWQWGPTQQPVVLP 125 Query: 144 DLLQGATNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGI 203 D L+G +L + + YR +++ +IV A +IE+TR GA +RA +N GI Sbjct: 126 DWLRGQISLLGLEVGAYRVFLIGVVVIVTVALGLLIERTRFGAQIRASVDNQVAAAGLGI 185 Query: 204 NVPLMVTLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIIT 263 NV + +LT+ G LA G L V+ + P ++ VV +GG G+I G ++ Sbjct: 186 NVSRVFSLTFALGSGLAGLGGGLGIDVLGLDPTFPVKYMVYFLLVVAVGGAGTIKGPLLA 245 Query: 264 GLVLGLIEGLTRVFYPELSATVVFIIMAIVLMVRPAGLFGKE 305 L+LG+ + + + PE+ A V++ +M ++L++ PAGL + Sbjct: 246 ALILGVFDVAGKYYVPEIGAFVIYGLMVVLLILFPAGLMRRR 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.331 0.147 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 734,710 Number of extensions: 25899 Number of successful extensions: 226 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 24 Length of query: 306 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 221 Effective length of database: 1,217,395 Effective search space: 269044295 Effective search space used: 269044295 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)