BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0513.p
         (210 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba...   411   e-116
gi|300312440|ref|YP_003776532.1| threonine efflux protein [Herba...    86   2e-18
gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba...    57   1e-09

>gi|300314064|ref|YP_003778156.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 210

 Score =  411 bits (1057), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 210/210 (100%), Positives = 210/210 (100%)

Query: 1   MQALFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVL 60
           MQALFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVL
Sbjct: 1   MQALFHSLGITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVL 60

Query: 61  MFLAAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAEFRRG 120
           MFLAAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAEFRRG
Sbjct: 61  MFLAAAGVAALLHANPLLFHGLQYLGAAYLAYLGARLLFARGEGGGTVVPFSNAAEFRRG 120

Query: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180
           FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR
Sbjct: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180

Query: 181 RMARNRTIARLASRAAGVFLIGFGIKLTTQ 210
           RMARNRTIARLASRAAGVFLIGFGIKLTTQ
Sbjct: 181 RMARNRTIARLASRAAGVFLIGFGIKLTTQ 210


>gi|300312440|ref|YP_003776532.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 208

 Score = 85.9 bits (211), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 68/207 (32%), Positives = 91/207 (43%), Gaps = 12/207 (5%)

Query: 10  ITDIWQLIAATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAAGVA 69
           + D+   + A M   L PGP    VLT     G + G  A AG   G V    LA  G A
Sbjct: 1   MPDLILFLLAAMTLTLAPGPDNLYVLTRGIAQGRKAGLVAAAGFCSGLVFHTLLAVLGFA 60

Query: 70  ALLHANPLLFHGLQYLGAAYLAYLGARLL---------FARGEGGGTVVPFSNAAEFRRG 120
           AL+ A P  +H LQY GAAYLAYLG R L          A G+G    VP      + + 
Sbjct: 61  ALIKAYPPAYHALQYAGAAYLAYLGVRTLRSASAGLALAADGQGAAA-VPLRRI--YWQS 117

Query: 121 FLVTLFNPKAIVFYMAFFPLFLDPATQQGALTFITMGAVISCCTFLYGSVLVMVGNAAAR 180
            L  + NPK  +F++AF P F+       AL  + +  V     F   S + +   A   
Sbjct: 118 VLANMLNPKVTLFFIAFLPQFVQREAGHEALQMLVLALVFIVQAFAIFSAIALFSGAVGA 177

Query: 181 RMARNRTIARLASRAAGVFLIGFGIKL 207
              R  + +   +R AG   IG GI++
Sbjct: 178 YFRRRASASVHLNRLAGCAFIGLGIRM 204


>gi|300311201|ref|YP_003775293.1| threonine efflux protein
           [Herbaspirillum seropedicae SmR1]
          Length = 219

 Score = 56.6 bits (135), Expect = 1e-09,   Method: Compositional matrix adjust.
 Identities = 61/184 (33%), Positives = 84/184 (45%), Gaps = 26/184 (14%)

Query: 18  AATMVFLLLPGPGTFCVLTCAAKGGVRGGFAAQAGLMLGDVVLMFLAAAGVAALLHANPL 77
           AA  V  + PG G    ++     GVR   A   GL +  VV++ +A AGV +LL A+  
Sbjct: 11  AAACVIAISPGSGAVLSMSHGLNYGVRRTSATILGLEIALVVILIIAGAGVGSLLVASET 70

Query: 78  LFHGLQYLGAAYLAYLGARLLFAR--GEGGGTVVP--------FSNAAEFRR---GFLVT 124
            F+ ++  GA YL YLG     AR   E  G  +          +    ++R   GFL  
Sbjct: 71  AFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHARQTWWQRCMTGFLTN 130

Query: 125 LFNPKAIVFYMAFFPLFLD---PATQQGALTFITMGAVISCCTFLYGSVLVMVGNA-AAR 180
             NPK IVF +A  P F+D   P   Q  +  +TM AV          ++VM G A AA 
Sbjct: 131 ATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAV---------DLVVMHGYAFAAS 181

Query: 181 RMAR 184
           R+ R
Sbjct: 182 RLQR 185


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.332    0.144    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 500,424
Number of extensions: 16099
Number of successful extensions: 77
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 74
Number of HSP's successfully gapped: 4
Length of query: 210
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 129
Effective length of database: 1,236,335
Effective search space: 159487215
Effective search space used: 159487215
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 128 (53.9 bits)