BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0510.p (531 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis... 1061 0.0 gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesi... 466 e-132 gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesi... 299 3e-82 >gi|300314061|ref|YP_003778153.1| periplasmic glucan biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 517 Score = 1061 bits (2745), Expect = 0.0, Method: Compositional matrix adjust. Identities = 515/517 (99%), Positives = 515/517 (99%) Query: 15 MASATLSACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYM 74 M SATLSACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYM Sbjct: 1 MVSATLSACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYM 60 Query: 75 KIQPKSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDLNL 134 KIQPKSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDLNL Sbjct: 61 KIQPKSEKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNFGDLNL 120 Query: 135 AKDSTAHLGYAGFRVVYPINRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMM 194 AKDSTAHLGYAGFRVVYPINRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMM Sbjct: 121 AKDSTAHLGYAGFRVVYPINRDDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMM 180 Query: 195 SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRG 254 SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRG Sbjct: 181 SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRG 240 Query: 255 EVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVS 314 EVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVS Sbjct: 241 EVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVS 300 Query: 315 SFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETN 374 SFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETN Sbjct: 301 SFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETN 360 Query: 375 DNIVAYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRA 434 DNIVAYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRA Sbjct: 361 DNIVAYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRA 420 Query: 435 FDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNGELIDAQLQPNPAINGWRLTLRVKIR 494 FDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNGELID QLQPNPAINGWRLTLRVKIR Sbjct: 421 FDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNGELIDVQLQPNPAINGWRLTLRVKIR 480 Query: 495 NVAQAVELRAALSANGKPLTETWSYQLPPRSDVQRTF 531 NVAQAVELRAALSANGKPLTETWSYQLPPRSDVQRTF Sbjct: 481 NVAQAVELRAALSANGKPLTETWSYQLPPRSDVQRTF 517 >gi|300312745|ref|YP_003776837.1| periplasmic glucans biosynthesis G protein [Herbaspirillum seropedicae SmR1] Length = 541 Score = 466 bits (1198), Expect = e-132, Method: Compositional matrix adjust. Identities = 236/490 (48%), Positives = 313/490 (63%), Gaps = 7/490 (1%) Query: 37 FDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYMKIQPKSEKFEWRDQKTPFKLAF 96 FD N ARAK LA K Y+KP L + + + +I + +K WR QK PF+L+F Sbjct: 55 FDFNTVAARAKALAGKSYKKPADELPKSLKDLSYEQASQIYFRDDKSYWRAQKLPFELSF 114 Query: 97 YHQGMQFNAPVAIHEIIDGRVQDVGYSTDRFNF-GDLNLAKDSTAHLGYAGFRVVYPINR 155 H G + P+ ++E++ V+++ ++ F++ ++ S +LG+AGFRV +P+N Sbjct: 115 IHLGASYTEPIKVNEVVGDAVREIHFNPALFDYSATRSVDPRSLRNLGFAGFRVRFPVNS 174 Query: 156 DDKEDEVMSVLGASYFRVIGKGQVYGLSARGLAIDTAMMSGEEFPHFTEFWIQRPAPGEK 215 +D+V++ GASYFR +GKGQ YGLSARGLAIDTA+ SGEEFP FTEFW++RP+ K Sbjct: 175 AKVKDDVLTFHGASYFRALGKGQSYGLSARGLAIDTALYSGEEFPRFTEFWLERPSAESK 234 Query: 216 QLTFYALLDSPRATGAYKFTLTPGKDALLDVQARVFLRGEVGRLGIAPLTSMFLFGPNQQ 275 +L YALLDSPRATGAY+F L PG D ++DV+A+++LR V +LGIAPLTSMFLFG NQ Sbjct: 235 ELVIYALLDSPRATGAYRFVLKPGADTVMDVKAQLYLRSNVTKLGIAPLTSMFLFGENQP 294 Query: 276 SSRRNFRPAIHDSNGLAIHTGNGEWLWRPLNDPQNVEVSSFEVSNPRGFGLLQRGREFSA 335 +FRP +HDS+GLA+ +G GEWLWRPL +P+ + V+SF NP GFGL+QR R FS Sbjct: 295 GPADDFRPEVHDSDGLAMQSGTGEWLWRPLVNPKRLLVTSFSFDNPLGFGLMQRDRSFSH 354 Query: 336 YEDLKDRYDLRPSAWIEPKGDWGKGAVQLVEIPTADETNDNIVAYWLPAQLPARGTPLAY 395 YEDL Y RPSAW+EPKG WG G V+LV+IPT DETNDNIVAYW+P LP G P Sbjct: 355 YEDLDYNYQARPSAWVEPKGKWGSGRVELVQIPTPDETNDNIVAYWIPNTLPKPGQPFNV 414 Query: 396 DYRIHWTMDEPAILNNEVGWVKQTFHTDGELTQANLIRAFDGTTALLVDFTGGPLGALPA 455 +YR+ W D + +V QT G + D L +DF GG L LPA Sbjct: 415 EYRLSWQKDNEK--RPPLAYVTQTLFGHGYRPKQE--GKTDDVQQLSIDFDGGNLKKLPA 470 Query: 456 GTPVQPQVSVSNNGELIDAQLQPNPAINGWRLTLRVKIRNVAQAVELRAAL-SAN-GKPL 513 V+ V NG+L + N A GWR+ ++++ + VELR L +AN G L Sbjct: 471 NARVEGVVEADANGKLASVTTRKNEATGGWRVEVKLRRTEENKPVELRGFLRNANGGTTL 530 Query: 514 TETWSYQLPP 523 +ETWSY LPP Sbjct: 531 SETWSYILPP 540 >gi|300313221|ref|YP_003777313.1| periplasmic glucans biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 299 bits (766), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 190/525 (36%), Positives = 273/525 (52%), Gaps = 37/525 (7%) Query: 21 SACLLAASAQAQTSQGFDLNQAVARAKDLAAKPYQKPVSNLSPTFAAMQFADYMKIQPKS 80 S L A + Q F Q + RA+ +A+ PYQ + + + + + KI+ K Sbjct: 28 SGALAAQGIETSAPQPFSFAQLIERARQMASTPYQPAAAAPAAILDRIDYDAHGKIRFKP 87 Query: 81 EKFEWRDQKTPFKLAFYHQGMQFNAPVAIHEIIDG--------RVQDVGYSTDRFNFGDL 132 ++ + PF L F+H G F PV + +++G + +++ Y D F+ Sbjct: 88 DRAAFATGPGPFPLTFFHLGKFFRVPVRMF-LLEGAGDATAPTQARELRYRDDYFD---- 142 Query: 133 NLAKDSTAHL-----GYAGFRVVYPI-----NRDDKEDEVMSVLGASYFRVIGKGQVYGL 182 + DS AH G+AGFR +D ++++ ++ LGASYFR IG YGL Sbjct: 143 -MPADSPAHALPPGSGFAGFRFQESRLGDQQVKDWRKNDWVAFLGASYFRAIGDLYQYGL 201 Query: 183 SARGLAIDTAMM-SGEEFPHFTEFWIQRPAPGEKQLTFYALLDSPRATGAYKFTLTPGKD 241 SARG+AID A+ EEFP FT+F+ + APG + YALLD P TGAY+F + G+ Sbjct: 202 SARGVAIDVAVAGKSEEFPDFTQFFFEPGAPGSDTVVVYALLDGPSITGAYRFAMQRGQG 261 Query: 242 ALLDVQARVFLRGEVGRLGIAPLTSMFLFGPNQQSSRRNFRPAIHDSNGLAIHTGNGEWL 301 +++ ++FLR +V RLG+AP TSM+ + + + ++RP +HDS+GLAI G GE L Sbjct: 262 VTMEIDKQLFLRRDVERLGLAPATSMYWYSETVKGTGVDWRPEVHDSDGLAIWNGAGEHL 321 Query: 302 WRPLNDPQNVEVSSFEVSNPRGFGLLQRGREFSAYEDLKDRYDLRPSAWIEPKGDWGKGA 361 WRPLN+P SSF PRGFGL+QR R+F Y D Y+ RPS W+EP GDWG+G Sbjct: 322 WRPLNNPPRTIASSFADRQPRGFGLMQRDRDFDHYLD-GVHYERRPSLWVEPLGDWGEGQ 380 Query: 362 VQLVEIPTADETNDNIVAYWLPAQLPARGTPLAYDYRIHWTMDEPAILNNEVGWVKQTFH 421 VQL+E+ T DE +DNIVA W+P G YR+HW + P + V Sbjct: 381 VQLIELRTDDEIHDNIVAMWVPKAAARAGDRYQLRYRLHWQSETP-FPSALARCVATRLG 439 Query: 422 TDGELTQAN--LIRAFDGTTALLVDFTGGPLGALPAGTPVQPQVSVSNNG-ELIDAQLQP 478 G+ Q +R F +V+F G PL ALP G + +S S + + P Sbjct: 440 RGGQPGQPRPPQVRKF------MVEFKGPPLQALPFGVKPEAVISASRGSISYVYTEALP 493 Query: 479 NPAINGWRLTLRVKIRNVAQAVELRAALSANGKPLTETWSYQLPP 523 N WR + + VELR L G+ L+ETW +Q P Sbjct: 494 NGVPGHWRAQFDLTAEG-EEPVELRLFLRHQGQALSETWLFQYHP 537 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,693,415 Number of extensions: 72083 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 3 Length of query: 531 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 441 Effective length of database: 1,193,720 Effective search space: 526430520 Effective search space used: 526430520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)