BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0496.p (129 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrog... 256 3e-70 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 68 2e-13 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 67 4e-13 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 61 2e-11 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 58 3e-10 >gi|300314046|ref|YP_003778138.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 472 Score = 256 bits (655), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 126/128 (98%), Positives = 127/128 (99%) Query: 1 VVTGDAAIGQAFAALPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCR 60 VVTGDAAIGQAFAALPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCR Sbjct: 171 VVTGDAAIGQAFAALPFDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCR 230 Query: 61 LHSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAADVAQQLAQAMGRLYPRLHDNPDYTAI 120 LHSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAA VAQ+LAQAMGRLYPRLHDNPDYTAI Sbjct: 231 LHSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAAGVAQELAQAMGRLYPRLHDNPDYTAI 290 Query: 121 ISPRHRAR 128 ISPRHRAR Sbjct: 291 ISPRHRAR 298 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 68.2 bits (165), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 38/90 (42%), Positives = 53/90 (58%) Query: 18 DHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCRLHSSLQRIAFGKLLNAG 77 +H+ FTGS VG+ + + AA + TPVTLELGGKSP I+ + + L + + NAG Sbjct: 218 NHISFTGSPNVGKLVVRAAAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAG 277 Query: 78 QTCVAPDYLLVPRGQAADVAQQLAQAMGRL 107 QTC A LLV RG V ++L+ +L Sbjct: 278 QTCSAGSRLLVQRGAYEQVLERLSGLFSQL 307 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 67.4 bits (163), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 48/131 (36%), Positives = 64/131 (48%), Gaps = 3/131 (2%) Query: 1 VVTGDAAIGQAFAALP-FDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSC 59 +VTGD A G A A P + FTGST+VGR I + A + +TLELGGKSP I+ Sbjct: 218 IVTGDGATGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGKSLTLELGGKSPFIVFEDA 277 Query: 60 RLHSSLQRIAFGKLLNAGQTCVAPDYLLVPRGQAADVAQQLAQAMGRLY--PRLHDNPDY 117 + ++++ + N GQ C A LLV G +L M +L L D Sbjct: 278 DIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPLDKCSDM 337 Query: 118 TAIISPRHRAR 128 A+ISP R Sbjct: 338 GALISPVQLER 348 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 61.2 bits (147), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 30/70 (42%), Positives = 44/70 (62%) Query: 22 FTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCRLHSSLQRIAFGKLLNAGQTCV 81 FTGST VG+Q+A LA ++ T+ELGG +PA++ + + + +A K NAGQ CV Sbjct: 225 FTGSTPVGKQLAALAGQHMKRATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCV 284 Query: 82 APDYLLVPRG 91 +P LV +G Sbjct: 285 SPTRFLVQKG 294 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 57.8 bits (138), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 37/92 (40%), Positives = 49/92 (53%), Gaps = 1/92 (1%) Query: 6 AAIGQAFAALP-FDHLFFTGSTQVGRQIAQLAAANLTPVTLELGGKSPAILDRSCRLHSS 64 A +G A +A P D + TGST+ G +AQ AA + V ELGGKSP ++ L + Sbjct: 215 AEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELGGKSPNLVLPDADLERA 274 Query: 65 LQRIAFGKLLNAGQTCVAPDYLLVPRGQAADV 96 + L N GQ+C AP L+VPR A V Sbjct: 275 IPPGVAAALRNMGQSCSAPTRLIVPRAALARV 306 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 396,054 Number of extensions: 13840 Number of successful extensions: 64 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 62 Number of HSP's successfully gapped: 12 Length of query: 129 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 54 Effective length of database: 1,264,745 Effective search space: 68296230 Effective search space used: 68296230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 125 (52.8 bits)