BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0492.p (384 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 709 0.0 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 144 2e-35 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 129 5e-31 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 79 5e-16 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 709 bits (1831), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/388 (95%), Positives = 372/388 (95%), Gaps = 4/388 (1%) Query: 1 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAH 60 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAH Sbjct: 1 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAH 60 Query: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT Sbjct: 61 SQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 Query: 121 LMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA 180 LMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA Sbjct: 121 LMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA 180 Query: 181 LQARLRTLNPGARQLQPQVGRIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQAR---- 236 LQARLRTLNPGARQLQPQVG+IAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQAR Sbjct: 181 LQARLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQARHGHG 240 Query: 237 HEHAHHHAHDHHGHGHHHGHDHHDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV 296 H HH HHGH H H H HDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV Sbjct: 241 HHGHHHGHDHHHGHDHGHDHHGHDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLV 300 Query: 297 GFKGPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVERA 356 GFKGPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVERA Sbjct: 301 GFKGPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVERA 360 Query: 357 TIERSFDAFMQAQAIEREAREKKAAPSY 384 TIERSFDAFMQAQAIEREAREKKAAPSY Sbjct: 361 TIERSFDAFMQAQAIEREAREKKAAPSY 388 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 117/418 (27%), Positives = 186/418 (44%), Gaps = 95/418 (22%) Query: 7 AGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHS----- 61 A D +PV++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV Sbjct: 8 AADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADLA 67 Query: 62 -QEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT 120 EE +VEMS+GC+CCT+R DL ++ + R+F+++VIE+TG++ P P+ T Sbjct: 68 RTEEKLVEMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAET 121 Query: 121 L-MTD---AFIAERYRLDGVIVTVDAVNGMS------TLDHHEEA-------------VQ 157 TD A +++ RLD ++ VDA N + +LD ++ ++ Sbjct: 122 FTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLIE 181 Query: 158 QAAVADRLLLTKTDLVDAATLAALQARLRTLNPGARQLQPQVGRIAAAELLDAGLFKPGE 217 Q D ++L K DLV A L L A L LNP AR ++ + G++A ++LD GLF E Sbjct: 182 QIEFCDVIVLNKLDLVAADDLERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLFD-FE 240 Query: 218 KMPDVARWLNEEAFAEQARHEHAHHHAHDHHGHGHHHGHDHHDHEHDVNRHDDHIRAFCF 277 + WL E R EH + +G I +F + Sbjct: 241 QASQAPSWLQE------LRGEHVPE--TEQYG----------------------ISSFAW 270 Query: 278 TFDEPIDPVLFDEWLSLLVGFKGPNILRIKGILNLK---------GEALPTVIHGVQHIF 328 P+ P F + L+ + P ++R KG L +A HG+ + Sbjct: 271 QARRPLHPQRFFD----LINTEWPGVVRSKGFFWLASRPTQAGSWSQAGAVCRHGLAGKW 326 Query: 329 HPTATLPQWPSE----------------DRRSRIVFITRDVERATIERSFDAFMQAQA 370 QWP + D R +V I +++ + DA + + A Sbjct: 327 WAAVPREQWPQDQESVDFILAHWDENVGDARQELVLIGMAMDQEALRARLDACLLSDA 384 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 129 bits (323), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 83/237 (35%), Positives = 133/237 (56%), Gaps = 34/237 (14%) Query: 12 IPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHSQE-----ETI 66 IPV++L+GFLGSGKTTLLNH++ VI+N+ E+ +D V + E + + Sbjct: 17 IPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRNTELYRGNDEL 76 Query: 67 VEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHT---LMT 123 VEMS+GC+CCT+R DL + + + A GR F+ ++IE+TG++ P P+ T L + Sbjct: 77 VEMSNGCICCTLRADLLEQVSAL----ARSGR--FDYLLIESTGISEPIPVAETFAFLDS 130 Query: 124 DAF-IAERYRLDGVIVTVDA---------------VNGMSTLDHHEEAVQQAAVADRLLL 167 D F ++E RLD ++ V+ +G ST + ++Q A+ +L+ Sbjct: 131 DGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIEQVEYANVILV 190 Query: 168 TKTDLVDAATLAALQARLRTLNPGARQLQPQVGRIAAAELLDAGLFKPGEKMPDVAR 224 ++ DL+DA+ A+L+A L+ LNP AR L G + ++LD LF +P +AR Sbjct: 191 SRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLF----DLPSLAR 243 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 79.3 bits (194), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 80/276 (28%), Positives = 127/276 (46%), Gaps = 19/276 (6%) Query: 1 MNPSVPAGDAPIPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLD-HLLVA 59 + P++P PIPV+++TGFLG+GKTTLL L+ + + + ++INEFGE+ +D LL A Sbjct: 10 LAPALP----PIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLLRA 65 Query: 60 HSQEETIVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILH 119 + V++ GD + + A R + + V+IET+GLA P+ ++ Sbjct: 66 EGDAQGQVQIQDFAHGLIAYGDDAHFVPAMLALAAR--RAQIDHVLIETSGLALPSAVME 123 Query: 120 TLMTDAFIAERYRLDGVIVTVDAVNGMSTLDHHEEAV---------QQAAVADRLLLTKT 170 L A +A + LD + VD + +AV QQ AD ++L K Sbjct: 124 VLQ-GAEMARHFVLDATLAVVDTPLLLEDAFASADAVGAATAQLFRQQLECADVVVLNKI 182 Query: 171 DLVDAATLAALQARLRTLNPGARQLQPQVG-RIAAAELLDAGLFKPGEKMPDVARWLNEE 229 D ++ A L + +R L P R L+ G R+ L L + G + ++ Sbjct: 183 DGMEEAPLLQAEQAVRALAPNVRFLELAYGARLDIRLALGLRLHQAGATVHHHYTPVSMP 242 Query: 230 AFAEQARHEHAHHHAHDHHGHG-HHHGHDHHDHEHD 264 Q + + H H G G H HG H H H+ Sbjct: 243 GERAQVAPDQRRFNGHVHSGLGAHSHGLSTHKHFHE 278 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,004,740 Number of extensions: 36857 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 4 Length of query: 384 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 297 Effective length of database: 1,207,925 Effective search space: 358753725 Effective search space used: 358753725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)