BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0489.p (234 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314040|ref|YP_003778132.1| transcription regulator protein... 468 e-133 gi|300311999|ref|YP_003776091.1| GntR family transcription regul... 75 5e-15 gi|300311599|ref|YP_003775691.1| transcription regulator protein... 75 6e-15 gi|300310738|ref|YP_003774830.1| transcription regulator protein... 70 2e-13 gi|300313520|ref|YP_003777612.1| transcription regulator protein... 61 7e-11 >gi|300314040|ref|YP_003778132.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 468 bits (1204), Expect = e-133, Method: Compositional matrix adjust. Identities = 233/234 (99%), Positives = 233/234 (99%) Query: 1 MYKIDAPAQLIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREG 60 MYKIDAPAQLIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREG Sbjct: 1 MYKIDAPAQLIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREG 60 Query: 61 FIEDAGRKGVMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKSDAALRAAGEREGRALI 120 FIEDAGRKGVMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKSDAALRAAGER GRALI Sbjct: 61 FIEDAGRKGVMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKSDAALRAAGERAGRALI 120 Query: 121 EAGRALADMRDLSARILADMDFHQFIYRSCGNPMIGQTTALHWQHIRRAMGAILSSEVRS 180 EAGRALADMRDLSARILADMDFHQFIYRSCGNPMIGQTTALHWQHIRRAMGAILSSEVRS Sbjct: 121 EAGRALADMRDLSARILADMDFHQFIYRSCGNPMIGQTTALHWQHIRRAMGAILSSEVRS 180 Query: 181 HLAVWEEHEAILQAMLEGRPALADKLARQHAESAAAHLARVLTSAHYADKRRQA 234 HLAVWEEHEAILQAMLEGRPALADKLARQHAESAAAHLARVLTSAHYADKRRQA Sbjct: 181 HLAVWEEHEAILQAMLEGRPALADKLARQHAESAAAHLARVLTSAHYADKRRQA 234 >gi|300311999|ref|YP_003776091.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 222 Score = 75.1 bits (183), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 64/227 (28%), Positives = 104/227 (45%), Gaps = 15/227 (6%) Query: 4 IDAPAQLIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREGFIE 63 +D P E + +I + I L+P + + + LAE+ GVSR PI +A L G +E Sbjct: 1 MDTPKTRAEALRESIEELIAVSKLSPGQHLDETSLAEEFGVSRTPIREALIQLASMGIVE 60 Query: 64 DAGRKGVMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKSDAALRAAGEREGRALIEAG 123 R+G +V + PQ+L+++++V A + + R A + + A E A +A Sbjct: 61 MRPRRGAIVAEIGPQQLIEMFEVMAEFEAMCGRLAARR------MTPAEHAELLAAHQAC 114 Query: 124 RALADMRDLSARILADMDFHQFIYRSCGNPMIG-QTTALH--WQHIRRAMGAILSSEVRS 180 +A D D A + FH IY N + Q ALH + RR L VR Sbjct: 115 QAARDANDPDAYFYLNEAFHDHIYAGSHNGFLADQARALHRRLRPYRR-----LQLRVRD 169 Query: 181 HL-AVWEEHEAILQAMLEGRPALADKLARQHAESAAAHLARVLTSAH 226 L + ++EH+A++ A++ G L R+H A ++ S H Sbjct: 170 RLKSSFDEHQAVVDAIISGDAEKTVDLLREHIMIQGQRFADLVASLH 216 >gi|300311599|ref|YP_003775691.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 243 Score = 74.7 bits (182), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 58/204 (28%), Positives = 90/204 (44%), Gaps = 10/204 (4%) Query: 10 LIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREGFIEDAGRKG 69 L E V+ I AI G +AP KI++ LA G+SR P+ +A L+ + + G Sbjct: 17 LSEHVFRKIQSAIVQGEIAPGSKISEPELARTYGISRGPLREAIHRLEGQNLLVRVPHVG 76 Query: 70 VMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKS---DAALRAAGEREGRALIEAGRAL 126 V +L + L++L+Q+R L+G+A R A++ D R E +AGR Sbjct: 77 ARVVSLTLEGLVELFQIRESLEGMACRLAAQRMSRAELDELRRVLDTHEKDEAFQAGRGY 136 Query: 127 ADMRDLSARILADMDFHQFIYRSCGNPMIGQTTALHWQHIRRAMGAILSSEVRSHLAVWE 186 D DFH I ++ GN ++ + + R S+ + Sbjct: 137 YQQE-------GDYDFHYKIIQASGNQILTRILCGELYQLARMYRIQYSATPHRPRQAFA 189 Query: 187 EHEAILQAMLEGRPALADKLARQH 210 EH IL A+ +G LAD L R+H Sbjct: 190 EHHRILDALADGDGELADLLMRRH 213 >gi|300310738|ref|YP_003774830.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 234 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 63/216 (29%), Positives = 98/216 (45%), Gaps = 15/216 (6%) Query: 10 LIEQVYHAILDAICTGALAPNEKITQEGLAEQLGVSRQPILQAFQILKREGFIEDAGRKG 69 L EQVYH + D I + L P + T+ +A++ GVSR P+ A L+REG++E R+G Sbjct: 9 LAEQVYHRLKDDIFSFRLLPGDNFTETEMAQRQGVSRTPLRDALFRLQREGYLEVGFRRG 68 Query: 70 VMVTALDPQRLMQLYQVRAVLDGLAARSTAEQWKSDAALRAAGEREGRALIEAGRALADM 129 V +D RL LY +R VL+ A Q ++ A L L A D Sbjct: 69 WKVCPIDFDRLDNLYDLRIVLEMAALDKLCSQPEASAEL--------TQLNAIWLAEPDA 120 Query: 130 RDLSARILADMD--FHQFIYRSCGNPMIGQTTALHWQHIR--RAMGAILSSEVRSHLAVW 185 R A +A +D FH + + GN + + + IR R + + + A + Sbjct: 121 RQTDAVKVAQLDEAFHTNLVAAAGNEEMTRVHTEVTEKIRIVRRLDFLKKKRID---ATY 177 Query: 186 EEHEAILQAMLEGRPALADKLARQHAESAAAHLARV 221 EEH ILQ + + + A L R H + + ++ Sbjct: 178 EEHGKILQLIAKRKATEAQILLRAHITQSKLEVRKI 213 >gi|300313520|ref|YP_003777612.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 233 Score = 61.2 bits (147), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 71/228 (31%), Positives = 107/228 (46%), Gaps = 17/228 (7%) Query: 7 PAQ-LIEQVYHAILDAICTGALAPNEKITQEG-LAEQLGVSRQPILQAFQILKREGFIED 64 PAQ L + V +L+ I GA K+ E LA+Q GVSR + +A LK EG +E Sbjct: 6 PAQALSDTVARQLLENIDAGAFPRGSKLPTEAVLAQQFGVSRTVVREAIARLKHEGVVEP 65 Query: 65 AGRKGVMVT---ALDPQRL--MQLYQVRAVLDGLAARSTAEQWKSDAALRAAGEREGRAL 119 GV VT + P R+ ++ AVL + R E ++ A +AA R L Sbjct: 66 RQGSGVFVTEQAGIKPLRIDYTEVSSPEAVLQIVELRRAIE---AEVAAQAAKRRSAEDL 122 Query: 120 IEAGRALADMR-DLSA---RILADMDFHQFIYRSCGNPMIGQTTALHWQHIRRAMGAILS 175 ALA + D++A + AD+ FH+ + ++ NP +T Q++ A Sbjct: 123 QAIEAALAQIGVDVAAGGDGVAADVAFHRALAQATRNPYFIKTLEFLSQYLEAATAVTRG 182 Query: 176 SEVRSH---LAVWEEHEAILQAMLEGRPALADKLARQHAESAAAHLAR 220 +E R V EEHEAI++A+ G A A+ H +AA L++ Sbjct: 183 NEARYEDFSRQVREEHEAIVEAIRAGDEMAARNAAQTHMFNAARRLSQ 230 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 656,153 Number of extensions: 24357 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 11 Length of query: 234 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 152 Effective length of database: 1,231,600 Effective search space: 187203200 Effective search space used: 187203200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)