BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0482.p (411 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 820 0.0 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 413 e-116 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 394 e-111 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 64 2e-11 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 63 4e-11 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 820 bits (2118), Expect = 0.0, Method: Compositional matrix adjust. Identities = 408/411 (99%), Positives = 409/411 (99%) Query: 1 MSAHIPESIRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQ 60 MSAHIPE IRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQ Sbjct: 1 MSAHIPEPIRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQ 60 Query: 61 SGLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASEEQQR 120 SGLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLAS+EQQR Sbjct: 61 SGLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQR 120 Query: 121 LFFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWI 180 LFFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWI Sbjct: 121 LFFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWI 180 Query: 181 PSRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDA 240 PSRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDA Sbjct: 181 PSRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDA 240 Query: 241 LQQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIG 300 LQQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIG Sbjct: 241 LQQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIG 300 Query: 301 NLSLRLHAADALLEDAAALLDSLPAQLSFAQMAQASAAVAEAKVLSTELALLASEKLFEL 360 NLSLRLHAADALLEDAAALLDSLPAQLSF QMAQASAAVAEAKVLSTELALLASEKLFEL Sbjct: 301 NLSLRLHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALLASEKLFEL 360 Query: 361 SGSAATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 SGSAATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS Sbjct: 361 SGSAATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 413 bits (1062), Expect = e-116, Method: Compositional matrix adjust. Identities = 218/394 (55%), Positives = 274/394 (69%), Gaps = 2/394 (0%) Query: 15 RPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWAITVPRSHGG 74 R RI ++ A+ A +LA +F+ AA RDRER LP++++ER+ +SGLW ITVP+ +GG Sbjct: 27 RLVARIRDDAEAIAAARKLAEDFSREAALRDRERKLPWDELERYVRSGLWGITVPKEYGG 86 Query: 75 IGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASEEQQRLFFAEVLDGARLGN 134 G S+ T+AEVI ISAAD SIGQIPQNHF + LI SE Q+R FF VL G R GN Sbjct: 87 AGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGSESQKRFFFERVLQGDRFGN 146 Query: 135 AGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDDDGQPLVV 194 A E K D +TR L R A Y ++G +FY+TGALYA W+P+ IDDDG + Sbjct: 147 ALAELGTKTSHDRKTR--LSRNAHGPGYRIDGRKFYATGALYADWVPTSVIDDDGVQQLA 204 Query: 195 YTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQPNNIGPVSQLI 254 R APG+ +IDDWS FGQRTT SG+ VF+NV VPA V+ A +P IGP++Q+ Sbjct: 205 IVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRPTQIGPMAQIQ 264 Query: 255 QAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLHAADALLE 314 AAID GIA AA D + FV TRSR +VDSG+ERA DDP +R++G+L+++LHAA+ALL Sbjct: 265 HAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIREVGDLTIQLHAAEALLR 324 Query: 315 DAAALLDSLPAQLSFAQMAQASAAVAEAKVLSTELALLASEKLFELSGSAATLASHNLDR 374 A +D+ A +A AS AVAEA+VL+TE++L A KLFEL+GS +TLA HNLDR Sbjct: 325 RAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLAEHNLDR 384 Query: 375 HWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRH 408 HWRNAR HTLHDPVRWKY A+GNY LNG PPRH Sbjct: 385 HWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRH 418 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 394 bits (1012), Expect = e-111, Method: Compositional matrix adjust. Identities = 208/401 (51%), Positives = 269/401 (67%), Gaps = 5/401 (1%) Query: 11 RAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWAITVPR 70 RA Q PA I ++ A+ VA LA A A++RD+ R P E+IE +SQSGLWAI VPR Sbjct: 24 RASQ-PAHVIRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEIEAYSQSGLWAINVPR 82 Query: 71 SHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASEEQQRLFFAEVLDGA 130 + GG S+ T+AEVI +ISAADPS+GQI QNH G + + ++ EQQR FFA+ L G Sbjct: 83 AFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRTVSDPEQQRRFFADFLRGV 142 Query: 131 RLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDDDGQ 190 R GNA E K D TR AGD ++ G +FYSTGAL AH++P A D++ + Sbjct: 143 RWGNAFSESGSKRAADFETRFV---DAGD-HVIVTGKKFYSTGALLAHYVPIVANDEENR 198 Query: 191 PLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQPNNIGPV 250 V R+APG+ VIDDWS+FGQRTT SGTV+ D V+VP T ++ +P+ G + Sbjct: 199 TWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAYKGYLEPSADGAI 258 Query: 251 SQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLHAAD 310 Q+IQAA+D GIA AA+ DT FV T+SRP++DS + A +DPY ++ IG+L +RLHA++ Sbjct: 259 FQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQAIGDLQIRLHASE 318 Query: 311 ALLEDAAALLDSLPAQLSFAQMAQASAAVAEAKVLSTELALLASEKLFELSGSAATLASH 370 ALLE A +D+ A +A A AEAKVLSTE+A+ A+ KLFEL+G+ +TL + Sbjct: 319 ALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKLFELAGTRSTLGQY 378 Query: 371 NLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRHSWS 411 NLDRHWRNAR HTLHDPVRWKY VGNY LNG PP H+WS Sbjct: 379 NLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHAWS 419 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 88/361 (24%), Positives = 149/361 (41%), Gaps = 40/361 (11%) Query: 41 AAQRDRERILPFEQIERFSQSGLWAITVPRSHGGIGASWQTVAEVIRLISAADPSIGQI- 99 AA DR P E ++ ++ G + VP GG G + ++ + I+A D + I Sbjct: 26 AADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIV 85 Query: 100 -PQNHFGNLNLIARLASEEQQRLFFAEVLDGARLGNAG--PERNGKNVLDVRTRATLDRA 156 QN + + S Q+ + + G LG G + +RTRA D Sbjct: 86 SVQNSLA-CGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERD-- 142 Query: 157 AGDGSYLLNGTRFYSTGALYAHWIPSRAIDDD--GQPLV--VYTRRDAPGVRVIDDWSSF 212 GD ++LNGT+ + T +A A+ D G+ + D PG V Sbjct: 143 -GD-DFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTPGFVVGRSEEKM 200 Query: 213 GQRTTASGTVVFDNVRVPATQVL-----ALKDALQ--QPNNIGPVSQLIQAAIDAGIAHA 265 GQ + + ++ DN RVPAT +L + AL + IG +Q + G+A A Sbjct: 201 GQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSV------GMARA 254 Query: 266 AVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSLRLHAADALLEDAAALLDSLPA 325 A + +++ R V +A + + L D+ L L AA ++ AA L D Sbjct: 255 AFEAAVSYARQRESFGVPIIEHQAVN--FRLADMNTL---LDAARLMVWRAAQLKDQGRP 309 Query: 326 QLSFAQMAQASAAVAEAKVLSTELALLASEKLFELSGSAATLASHNLDRHWRNARVHTLH 385 L A MA+ A+ A K+ S + ++ G + ++R +R+ R+ ++ Sbjct: 310 CLKEASMAKMFASEAAEKIASDAI---------QIHGGVGYTSDFPVERIYRDVRICQIY 360 Query: 386 D 386 + Sbjct: 361 E 361 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 66/228 (28%), Positives = 95/228 (41%), Gaps = 37/228 (16%) Query: 29 VAAELAAEFATSAAQRDRERILPFEQIERFSQSGLWAITVPRSHGGIGASWQTVAEVIRL 88 VAA +E A AA+ DR P + ++ G+ ITV +GG G + + Sbjct: 22 VAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEE 81 Query: 89 ISAADPSIGQIPQNHFGNL--NLIARLASEEQQRLFFAEVLDGARLGN-AGPERN-GKNV 144 IS A S+G H NL N I R +EEQ+R + ++ G +G A E N G +V Sbjct: 82 ISRASASVGLSYGAH-SNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDV 140 Query: 145 LDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRAIDDDGQPLVVYTRRDA---- 200 + ++ RA GD Y+LNG++ WI + D LVVY + D Sbjct: 141 VSMKLRAD---KKGD-RYVLNGSKM---------WITN---GPDADVLVVYAKTDLEAGA 184 Query: 201 ------------PGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLA 236 G V G R + +G +VF + VP VL Sbjct: 185 RGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLG 232 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,138,485 Number of extensions: 43319 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 6 Length of query: 411 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 323 Effective length of database: 1,203,190 Effective search space: 388630370 Effective search space used: 388630370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)