BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0481.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 847 0.0 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 408 e-115 gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 400 e-112 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 88 1e-18 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 77 2e-15 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 847 bits (2187), Expect = 0.0, Method: Compositional matrix adjust. Identities = 420/421 (99%), Positives = 420/421 (99%) Query: 1 MTQTAAAHASLTTLDSFPPPPSGTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRER 60 MTQTAAAHASLTTLDSFPPPPS TPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRER Sbjct: 1 MTQTAAAHASLTTLDSFPPPPSSTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRER 60 Query: 61 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE 120 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE Sbjct: 61 KLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVE 120 Query: 121 LIRVNGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFY 180 LIRVNGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFY Sbjct: 121 LIRVNGSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFY 180 Query: 181 ATGALYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVP 240 ATGALYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVP Sbjct: 181 ATGALYADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVP 240 Query: 241 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAV 300 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAV Sbjct: 241 ADAVIGFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAV 300 Query: 301 DDPLTIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVS 360 DDPLTIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVS Sbjct: 301 DDPLTIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVS 360 Query: 361 LAAGSKLFELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA 420 LAAGSKLFELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA Sbjct: 361 LAAGSKLFELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHGA 420 Query: 421 I 421 I Sbjct: 421 I 421 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 408 bits (1048), Expect = e-115, Method: Compositional matrix adjust. Identities = 216/412 (52%), Positives = 278/412 (67%), Gaps = 4/412 (0%) Query: 8 HASLTTLDSFPPPPSGTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRERKLPWDEL 67 H++ + P P + P V IRDDAEAIA A LA ++ A+ RD+ R+ P +E+ Sbjct: 10 HSTSSAAAPVPGLPRASQPAHV--IRDDAEAIAVAHALAPRLAQGASERDQLRRWPVEEI 67 Query: 68 ERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGS 127 E Y +SGLW I VP+ +GGA VSY TLAEVIA ISAAD S+GQI QNH V +R Sbjct: 68 EAYSQSGLWAINVPRAFGGAEVSYATLAEVIAIISAADPSLGQITQNHLGFVAAVRTVSD 127 Query: 128 ESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGALYA 187 Q+R FF L+G R+GNA +E G+K + D +TR G + G+KFY+TGAL A Sbjct: 128 PEQQRRFFADFLRGVRWGNAFSESGSKRAADFETRFVDA--GDHVIVTGKKFYSTGALLA 185 Query: 188 DWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGF 247 +VP D++ + R+APGLT+IDDWS FGQRTT SG+ + + V VP +I Sbjct: 186 HYVPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPA 245 Query: 248 ATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIR 307 + P+ G + QI AA+D+GIARAA AD FVRT+SR W+DS + A +DP +I+ Sbjct: 246 YKGYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQ 305 Query: 308 EVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKL 367 +GDL I+LHA+EALL RAGR +DAA AAPD SVA A I AEA+VL+TE+++ A +KL Sbjct: 306 AIGDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKL 365 Query: 368 FELAGSQSTLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRHG 419 FELAG++STL ++NLDRHWRNARTHTLHDPVRWKY +GNY LNG KPP H Sbjct: 366 FELAGTRSTLGQYNLDRHWRNARTHTLHDPVRWKYAIVGNYYLNGVKPPFHA 417 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 400 bits (1028), Expect = e-112, Method: Compositional matrix adjust. Identities = 219/404 (54%), Positives = 277/404 (68%), Gaps = 2/404 (0%) Query: 17 FPPPPSGTPPRLVARIRDDAEAIAAARKLAEDFSREAALRDRERKLPWDELERYVRSGLW 76 P P R RI ++ A+ A +LA +F+ AA RDRER LP++++ER+ +SGLW Sbjct: 5 IPEPIRRAGQRPAQRISSESHALQVAAELAAEFATSAAQRDRERILPFEQIERFSQSGLW 64 Query: 77 GITVPKEYGGAGVSYGTLAEVIATISAADGSIGQIPQNHFYAVELIRVNGSESQKRFFFE 136 ITVP+ +GG G S+ T+AEVI ISAAD SIGQIPQNHF + LI S+ Q+R FF Sbjct: 65 AITVPRSHGGIGASWQTVAEVIRLISAADPSIGQIPQNHFGNLNLIARLASQEQQRLFFA 124 Query: 137 RVLQGDRFGNALAELGTKTSHDRKTR--LSRNAHGPGYRIDGRKFYATGALYADWVPTSV 194 VL G R GNA E K D +TR L R A Y ++G +FY+TGALYA W+P+ Sbjct: 125 EVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTRFYSTGALYAHWIPSRA 184 Query: 195 IDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRP 254 IDDDG + R APG+ +IDDWS FGQRTT SG+ VF+NV VPA V+ A +P Sbjct: 185 IDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVRVPATQVLALKDALQQP 244 Query: 255 TQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAVDDPLTIREVGDLTI 314 IGP++Q+ AAID GIA AA D + FV TRSR +VDSG+ERA DDP +R++G+L++ Sbjct: 245 NNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLERASDDPYILRDIGNLSL 304 Query: 315 QLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFELAGSQ 374 +LHAA+ALL A +D+ A + +A AS AVAEA+VL+TE++L A KLFEL+GS Sbjct: 305 RLHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTELALLASEKLFELSGSA 364 Query: 375 STLAEHNLDRHWRNARTHTLHDPVRWKYHAIGNYVLNGKKPPRH 418 +TLA HNLDRHWRNAR HTLHDPVRWKY A+GNY LNG PPRH Sbjct: 365 ATLASHNLDRHWRNARVHTLHDPVRWKYFAVGNYALNGTPPPRH 408 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 88.2 bits (217), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 98/384 (25%), Positives = 163/384 (42%), Gaps = 43/384 (11%) Query: 34 DDAEAIAAARKLAEDFSRE-----AALRDRERKLPWDELERYVRSGLWGITVPKEYGGAG 88 D E IAA R+ F+ AA DR + P D ++ G+ GITV +EYGGAG Sbjct: 10 DHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAG 69 Query: 89 VSYGTLAEVIAT--ISAADGSIGQIPQNHF-YAVELIRVNGSESQKRFFFERVLQGDRFG 145 + Y LA +IA IS A S+G H V I+ NG+E QKR + R++ GD G Sbjct: 70 LGY--LAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIG 127 Query: 146 NALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGALYADWVPTSVIDD--DGVQQL 203 S +L + G Y ++G K + T AD + D G + + Sbjct: 128 ALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLEAGARGM 187 Query: 204 A--IVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVIGFATAFSRPTQIGPMA 261 +V + G ++ G R + +G VF++ VP + V+G +G Sbjct: 188 TAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLG---------GVGRGV 238 Query: 262 QIQHAAID----------LGIARAAYADLLTFVRTRSRAWVDSGVERAVDD-PLTIREVG 310 + + +D LGI +A ++ +V R + +A+ + L ++ Sbjct: 239 NVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQ------FGQAIGEFQLMQGKLA 292 Query: 311 DLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGSKLFEL 370 D+ + A +A + G+ D A +A D V A A A + + E + + + Sbjct: 293 DMYSTMMACKAYVYAVGQACDRADSA---DKVRALRKDAAGAILYSAEKATWMAGEAIQS 349 Query: 371 AGSQSTLAEHNLDRHWRNARTHTL 394 G + E+ + R WR+A+ + + Sbjct: 350 LGGNGYINEYPVGRLWRDAKLYEI 373 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 77.0 bits (188), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 85/353 (24%), Positives = 151/353 (42%), Gaps = 34/353 (9%) Query: 57 DRERKLPWDELERYVRSGLWGITVPKEYGGAGVSYGTLAEVIATISAADGSIGQI--PQN 114 DR P + L+ G G+ VP+++GGAG+ Y +L + I+A DG+ I QN Sbjct: 30 DRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQN 89 Query: 115 HFYAVELIRVNGSESQKRFFFERVLQGDRFGN-ALAELGTKT-SHDRKTRLSRNAHGPGY 172 A + GS +QK + + + +G+ G L E T + + +TR R+ G + Sbjct: 90 SL-ACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRAERD--GDDF 146 Query: 173 RIDGRKFYATGALYADWVPTSVIDDDGVQQLAI----VPRHAPGLTIIDDWSGFGQRTTG 228 ++G K + T +A + D + I VP PG + GQ + Sbjct: 147 ILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTPGFVVGRSEEKMGQHASD 206 Query: 229 SGSAVFENVHVPADAVI-----GFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTF 283 + + +N VPA A++ G+ A S +I AA +G+ARAA+ +++ Sbjct: 207 TVQIMLDNCRVPATALLGKEGEGYRIALSNLEA----GRIGIAAQSVGMARAAFEAAVSY 262 Query: 284 VRTRSRAWVDSGVERAVDDPLTIREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVA 343 R R V +AV+ L D+ L AA ++ RA + D E S+ Sbjct: 263 ARQRESFGVPIIEHQAVNFRLA-----DMNTLLDAARLMVWRAAQLKDQGRPCLKEASM- 316 Query: 344 AASIAVAEARVLTTEVSLAAGSKLFELAGSQSTLAEHNLDRHWRNARTHTLHD 396 A++ +E + S ++ G ++ ++R +R+ R +++ Sbjct: 317 --------AKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYE 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,240,523 Number of extensions: 50339 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 6 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)