BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0479.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 923 0.0 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 257 1e-69 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 76 4e-15 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 75 7e-15 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 923 bits (2386), Expect = 0.0, Method: Compositional matrix adjust. Identities = 447/447 (100%), Positives = 447/447 (100%) Query: 1 MNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGVYDVYQGNTD 60 MNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGVYDVYQGNTD Sbjct: 13 MNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGVYDVYQGNTD 72 Query: 61 LTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVG 120 LTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVG Sbjct: 73 LTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVG 132 Query: 121 WNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYA 180 WNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYA Sbjct: 133 WNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYA 192 Query: 181 DPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSK 240 DPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSK Sbjct: 193 DPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQSK 252 Query: 241 DATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAH 300 DATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAH Sbjct: 253 DATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGLAH 312 Query: 301 YSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVG 360 YSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVG Sbjct: 313 YSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYASIVG 372 Query: 361 APDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGTLRE 420 APDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGTLRE Sbjct: 373 APDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQGTLRE 432 Query: 421 KLFQRGATLPQEHVGAGFRVGAAVEHA 447 KLFQRGATLPQEHVGAGFRVGAAVEHA Sbjct: 433 KLFQRGATLPQEHVGAGFRVGAAVEHA 459 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 257 bits (656), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 160/430 (37%), Positives = 226/430 (52%), Gaps = 30/430 (6%) Query: 8 NHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGV---------YDVYQGN 58 + LW HP+ R + ++ Y+ +AR LERGKFD LF AD + V + +Y G+ Sbjct: 15 SQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYIGD 74 Query: 59 TDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGR 118 D T DPL ++ A+AA T H+G G T + TY P+ AR +TLDHLS GR Sbjct: 75 QDATRL--------DPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGR 126 Query: 119 VGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRV 178 WN+VT A GL E L HD RYDRADEF+EV +KLW SW+ A+ + R Sbjct: 127 AAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWH-SWQPDALRLQQDR-- 183 Query: 179 YADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHAECVFISSQ 238 YAD KV I H G ++ V G S Q PV+ QAGSS RGQ F AR AE VF Sbjct: 184 YADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQP 243 Query: 239 SKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYARYASPEAGL 298 + + +++ A + GR P I+I + G++ Q A+E + A P GL Sbjct: 244 DLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPLVGL 303 Query: 299 AHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLELGGRYA-- 356 + S+ +DFS + LD P+ + + I+ L K++ + + L + G YA Sbjct: 304 STLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRA----ISAERQLTLADAGRLYAQG 359 Query: 357 ----SIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKRE 412 I G VAD L+S + G DG+ ++ + P F+DFV+ VIP LQ RG+++++ Sbjct: 360 VLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQD 419 Query: 413 YAQGTLREKL 422 YA LR+ L Sbjct: 420 YAGRQLRQHL 429 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 76.3 bits (186), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 70/307 (22%), Positives = 136/307 (44%), Gaps = 51/307 (16%) Query: 101 PFLFARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKL 160 P + A++++T+DH+S GR+ N+V+G+ +A+G LEHD+RY R++EF+ + + Sbjct: 106 PAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSNEFIRALKGI 164 Query: 161 WEGSWEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLS-EPSRQRTPVLYQAGSS 219 W +D + GD+Y LS +P ++ P ++Q GSS Sbjct: 165 WT---QDDFTFK-------------------GDFYRFNNYTLSPKPVQKPHPEIFQGGSS 202 Query: 220 GRGQAFGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQ 279 + A ++ F + + + + + I+ A + G ++I + V+ TE+ Sbjct: 203 RAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHK---VRIGVNAFVIARDTEE 259 Query: 280 QAQERYREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELN 339 +AQ ++ +A EA H A +A++ K + E N Sbjct: 260 EAQAVLQDIIDHAHVEA--VH--------------------AFGDAVKQAGKASPEGEGN 297 Query: 340 WTKRKLLQQLELG-GRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 398 W K ++ G +++G P ++A+ + + A G+D E E F + Sbjct: 298 WAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVALKA-IGVDLVLTGFLHFIEEVEYFGQK 356 Query: 399 VIPVLQE 405 V+P+++E Sbjct: 357 VLPLVRE 363 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 75.5 bits (184), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 83/347 (23%), Positives = 143/347 (41%), Gaps = 46/347 (13%) Query: 73 DPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMSTLDHLSRGRVGWNIVTGYLDSAA 132 DP ++ ++ VT+ + V + P AR+ +TLD GR+ N+V G + Sbjct: 57 DPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATEL 116 Query: 133 RAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAVLRDRARRVYADPAKVHKIAHHG 192 A G+ L+HD+RY+ ADE++ + KL +G + + G Sbjct: 117 EADGVF--LKHDERYEAADEYLTIWRKLLQG----------------------ETVNYEG 152 Query: 193 DYYHVEGIHLSEPSRQRT-PVLYQAGSSGRGQAFGARHAECVFISSQSKDATRKLVQSIR 251 ++ + L P+ Q+ P LY GSS A+H + + A R+ + +R Sbjct: 153 KHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVR 212 Query: 252 DGAVREGRDPDAIKIFMGLTVVTGRTEQQA-QERYREYARYASPEAGLAHYSAGTGIDFS 310 + A R+GR ++ + L + T +A R ++ + A Sbjct: 213 ERAARQGR---TVRFGVRLHAIVRETTAEAWTAAERLISKLTDEDIARAQ---------Q 260 Query: 311 QYELDEPIRPAKTNAIES--LTKVVTQPELNWTKRKLLQQLELGGRYASIVGAPDRVADE 368 Y + + + A+ K+ P L W L++ GG S+VG VA+ Sbjct: 261 NYARMDSVGQQRMAALHGGRRDKLEIAPNL-WAGVGLVR----GGAGTSLVGDATTVAER 315 Query: 369 LESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYKREYAQ 415 L+ + AD G+D + L+ E F ELV P+L + R+ AQ Sbjct: 316 LQEY-ADLGVDTFVLSGYPHLEEAIRFAELVFPLLGKNAVTLRDRAQ 361 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,538,716 Number of extensions: 69280 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 4 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)