BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0469.p (179 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 377 e-106 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 233 7e-63 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 166 7e-43 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 157 6e-40 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 143 8e-36 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 377 bits (969), Expect = e-106, Method: Compositional matrix adjust. Identities = 179/179 (100%), Positives = 179/179 (100%) Query: 1 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 60 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY Sbjct: 223 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 282 Query: 61 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 120 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV Sbjct: 283 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 342 Query: 121 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL 179 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL Sbjct: 343 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL 401 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 233 bits (594), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 101/173 (58%), Positives = 134/173 (77%) Query: 1 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 60 V+ H + GH +LQ++ GIG+ LDVSLHHHEK+DG+GYP+ L IS+ A+MGA+CDVY Sbjct: 226 VQSHPLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVY 285 Query: 61 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 120 DAITS+RPYK+GWDP+RSI+HMA S GHFD +QAFVKA+GIYP GSCV++Q+G L +V Sbjct: 286 DAITSDRPYKSGWDPSRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMV 345 Query: 121 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLAHSSDKITAYADPAKWGIGNL 173 ++QRPD LL P V++FYST ++ + +IDL S++KI DP KWG+ L Sbjct: 346 LEQRPDSLLAPLVRIFYSTNKKIAVTPTLIDLRRSANKIVGAVDPEKWGVTRL 398 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 166 bits (421), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 81/153 (52%), Positives = 103/153 (67%) Query: 1 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 60 ++ H G+ L+ G+ + LDV LHHHEKIDGSGYP + + IS+ ++M AVCDVY Sbjct: 214 IQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVY 273 Query: 61 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 120 DA+TS RPYK+GWDPA S+ MA+ GHFD AFVKA+GIYP GS V LQSGR +V Sbjct: 274 DAVTSERPYKSGWDPAESLSRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALV 333 Query: 121 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLA 153 + Q L P VK F +T + +ESEIIDL+ Sbjct: 334 IRQNHVDLTKPVVKAFVTTPSIEVVESEIIDLS 366 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 157 bits (396), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 80/181 (44%), Positives = 112/181 (61%), Gaps = 2/181 (1%) Query: 1 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 60 ++ H G+ L Q + + A DV LHHHEKIDG+GYP L+A +I++ ++M A+CDVY Sbjct: 220 IKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVY 279 Query: 61 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 120 DA+TS+RPYK WDPA +I MA+ GHFD AFVK +GIYP GS V L+SGR G V Sbjct: 280 DALTSDRPYKRAWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFV 339 Query: 121 VDQRPDQLLTPRVKVFYSTANRMPLESEIIDLAHS--SDKITAYADPAKWGIGNLPGIEL 178 + Q L P +KVF+ + ++EI+DL + +D I A + +L ++L Sbjct: 340 IRQNAQDLTRPVLKVFHYDDSMTVTDTEILDLLANPEADTIVARGGEEWARLSHLDAMQL 399 Query: 179 L 179 L Sbjct: 400 L 400 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 143 bits (361), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 71/137 (51%), Positives = 89/137 (64%) Query: 1 VRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVY 60 ++ H G+ L Q + A DV HHHE++DG GYP L A+SI++ A+M AVCDVY Sbjct: 217 IKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVY 276 Query: 61 DAITSNRPYKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVV 120 DA+TS RPYK GWDPA +I MA GHFD + AFVK +GIYP GS V L+SG GVV Sbjct: 277 DALTSERPYKHGWDPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVV 336 Query: 121 VDQRPDQLLTPRVKVFY 137 + Q P L P +KV + Sbjct: 337 IRQNPADLTRPVLKVLH 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 591,103 Number of extensions: 23052 Number of successful extensions: 74 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 75 Number of HSP's successfully gapped: 8 Length of query: 179 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 100 Effective length of database: 1,245,805 Effective search space: 124580500 Effective search space used: 124580500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)