BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0466.p (401 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 827 0.0 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 483 e-137 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 336 2e-93 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 316 2e-87 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 293 2e-80 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 400/401 (99%), Positives = 400/401 (99%) Query: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR Sbjct: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 Query: 61 DVTREELEEDDADQDAPAAPPADAATRNPASRTDTAIERERAQRIISSSKSTVLHMFSEA 120 DVTREELEEDDADQDAPAAPPADAA RNPASRTDTAIERERAQRIISSSKSTVLHMFSEA Sbjct: 61 DVTREELEEDDADQDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMFSEA 120 Query: 121 RMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQL 180 RMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQL Sbjct: 121 RMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQL 180 Query: 181 GMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGI 240 GMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGI Sbjct: 181 GMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGI 240 Query: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS Sbjct: 241 GEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARS 300 Query: 301 IRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYS 360 IRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYS Sbjct: 301 IRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYS 360 Query: 361 TANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL 401 TANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL Sbjct: 361 TANRMPLESEIIDLAHSSDKITAYADPAKWGIGNLPGIELL 401 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 483 bits (1243), Expect = e-137, Method: Compositional matrix adjust. Identities = 235/398 (59%), Positives = 293/398 (73%), Gaps = 3/398 (0%) Query: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 MLK I QLR+GMYI S G W P RK F +E +DL L+ PI I I+ AKG Sbjct: 1 MLKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGA 60 Query: 61 DVTREELEEDDADQDA---PAAPPADAATRNPASRTDTAIERERAQRIISSSKSTVLHMF 117 DV +E E+ + + + A A RTD ERERA RIISSS+ TVL MF Sbjct: 61 DVQTDEAEQGNGVESSLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLTMF 120 Query: 118 SEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLA 177 S+ARMGK IDVK+A++LV EI ASVSRNADA+ISL RLKT D+YTY+HSVAVCAMMISLA Sbjct: 121 SQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLA 180 Query: 178 NQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQI 237 +QLG++ Q+ +QAGMAGLLHD+GKM VP ILNKP+KLTEEEF SV+ H + GH +LQ++ Sbjct: 181 DQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQEL 240 Query: 238 EGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDP 297 GIG+ LDVSLHHHEK+DG+GYP+ L IS+ A+MGA+CDVYDAITS+RPYK+GWDP Sbjct: 241 PGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDP 300 Query: 298 ARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKV 357 +RSI+HMA S GHFD +QAFVKA+GIYP GSCV++Q+G L +V++QRPD LL P V++ Sbjct: 301 SRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRI 360 Query: 358 FYSTANRMPLESEIIDLAHSSDKITAYADPAKWGIGNL 395 FYST ++ + +IDL S++KI DP KWG+ L Sbjct: 361 FYSTNKKIAVTPTLIDLRRSANKIVGAVDPEKWGVTRL 398 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 336 bits (861), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 172/374 (45%), Positives = 232/374 (62%), Gaps = 11/374 (2%) Query: 2 LKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGRD 61 LK I + QL +GMY G WI PF R SF V ++ R I+T +G D Sbjct: 4 LKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQGID 63 Query: 62 VTREELEEDDADQDAPAAPPADAATRNPASRTDTAIERERAQRIISSSKSTVLHMFSEAR 121 + E AD+ P A + + S D A+ R+ ++K T MF +AR Sbjct: 64 LAGAE-----ADRLPETEEPEAAGSSSSRSELDDAL------RLYENAKQTTQQMFKQAR 112 Query: 122 MGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLANQLG 181 +GK +DV++ LV EI SV+R+++AL+S+ RLK D+YTYLHS+AVC +M++LA LG Sbjct: 113 LGKTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLG 172 Query: 182 MTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQIEGIG 241 M K+AG+AG LHDVGK +PL+ILNKP +L+E+E+ ++ H G+ L+ G+ Sbjct: 173 MDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVP 232 Query: 242 EVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDPARSI 301 + LDV LHHHEKIDGSGYP + + IS+ ++M AVCDVYDA+TS RPYK+GWDPA S+ Sbjct: 233 DYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESL 292 Query: 302 RHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKVFYST 361 MA+ GHFD AFVKA+GIYP GS V LQSGR +V+ Q L P VK F +T Sbjct: 293 SRMASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTT 352 Query: 362 ANRMPLESEIIDLA 375 + +ESEIIDL+ Sbjct: 353 PSIEVVESEIIDLS 366 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 316 bits (809), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 162/384 (42%), Positives = 229/384 (59%), Gaps = 23/384 (5%) Query: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 MLK I ++ L LGMY W+ HPF + F ++ +L+ I I+T +G Sbjct: 1 MLKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGL 60 Query: 61 DVTREELEEDDA----------DQDAPAAPPADAATRNPASRTDTAIERERAQRIISSSK 110 DV +EE D A + + AAPP DA + + +TA + +A Sbjct: 61 DVAQEEEAADSAAPLQETEPSPEPEHAAAPPIDAVLLDAMALRETAKKATKA-------- 112 Query: 111 STVLHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVC 170 MFSEARMGK +D++ +V EI SV ++ + +S+ RLK D+YTYLHSVAVC Sbjct: 113 -----MFSEARMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVC 167 Query: 171 AMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQG 230 A+M++L LG+ ++AG+ G LHDVGK +P ++LNKP KL++EEF+ ++ H G Sbjct: 168 ALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELG 227 Query: 231 HGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRP 290 + L Q + + A DV LHHHEKIDG+GYP L+A +I++ ++M A+CDVYDA+TS+RP Sbjct: 228 YKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRP 287 Query: 291 YKAGWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQL 350 YK WDPA +I MA+ GHFD AFVK +GIYP GS V L+SGR G V+ Q L Sbjct: 288 YKRAWDPAEAISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDL 347 Query: 351 LTPRVKVFYSTANRMPLESEIIDL 374 P +KVF+ + ++EI+DL Sbjct: 348 TRPVLKVFHYDDSMTVTDTEILDL 371 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 293 bits (749), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 161/366 (43%), Positives = 211/366 (57%), Gaps = 20/366 (5%) Query: 1 MLKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGR 60 MLK I + L LGMY W++HPF + F + DLQ R I I+ ++GR Sbjct: 1 MLKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGR 60 Query: 61 DVTREELEEDDA-------DQDAPAAPPADAATRNPASRTDTAIERERAQRIISSSKSTV 113 DV + +A DQ A ADA R RE A+ + Sbjct: 61 DVAGAAPAKAEAAAAPASPDQSAATHAGADA------DRASAVALREHAREVTR------ 108 Query: 114 LHMFSEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMM 173 MF+ ARMGK +++ LV EI SV R+ + L+S+ RLK D+YTYLHSV+VCA++ Sbjct: 109 -QMFNAARMGKMVELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALV 167 Query: 174 ISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGI 233 I L LG++ ++AG++G LHDVGK + +ILNKP KLT EEFA ++ H G+ Sbjct: 168 IGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQY 227 Query: 234 LQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKA 293 L Q + A DV HHHE++DG GYP L A+SI++ A+M AVCDVYDA+TS RPYK Sbjct: 228 LIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKH 287 Query: 294 GWDPARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTP 353 GWDPA +I MA GHFD + AFVK +GIYP GS V L+SG GVV+ Q P L P Sbjct: 288 GWDPAEAISQMARWEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRP 347 Query: 354 RVKVFY 359 +KV + Sbjct: 348 VLKVLH 353 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,150,350 Number of extensions: 44409 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 11 Length of query: 401 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 314 Effective length of database: 1,207,925 Effective search space: 379288450 Effective search space used: 379288450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)