BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0465.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 923 0.0 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 300 2e-82 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 120 2e-28 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 109 6e-25 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 107 2e-24 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 923 bits (2385), Expect = 0.0, Method: Compositional matrix adjust. Identities = 474/475 (99%), Positives = 474/475 (99%) Query: 1 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD Sbjct: 1 MSSQPTAPGTAAPSPHIVIAGEGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALD 60 Query: 61 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP 120 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP Sbjct: 61 LQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLP 120 Query: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA Sbjct: 121 TLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILA 180 Query: 181 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH 240 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH Sbjct: 181 IAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLARHAFDLRSALLNAAAFGLLILAIGEGAH 240 Query: 241 QTQASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA 300 Q QASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA Sbjct: 241 QAQASVVGLELAAAILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLA 300 Query: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAG 360 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAG Sbjct: 301 FVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAG 360 Query: 361 LLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 LLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL Sbjct: 361 LLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLL 420 Query: 421 GQSVGAALVALCFNLSQTQGSRLALGFGAAFAGAACIASFLRLLTRNPYAPPPKK 475 GQSVGAALVALCFNLSQTQGSRLALGFGAAFAGAACIASFLRLLTRNPYAPPPKK Sbjct: 421 GQSVGAALVALCFNLSQTQGSRLALGFGAAFAGAACIASFLRLLTRNPYAPPPKK 475 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 300 bits (767), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 184/418 (44%), Positives = 261/418 (62%), Gaps = 8/418 (1%) Query: 22 EGLPPEQRGRAILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMV 81 EGLP +QR RA+LA+ A +A D I N ALP ++ LQT+ A S+W+ AY LA + Sbjct: 20 EGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAM 79 Query: 82 ALLPLAALGEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNT 141 L AAL IG+R + GL +FT ASL CA++ S+ L++ R LQGLGGA ++S+ Sbjct: 80 TLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAP 139 Query: 142 ALIRFIYPTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVL 201 A+ R I+P R LGR +GLN ++VA + VGP + +LA+ W W+FA+NVPLGVLAV L Sbjct: 140 AIHRTIFPNRLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWL 199 Query: 202 AVPSLPHTSL-ARHAFDLRSALLNAAAFGLLILAIGEGAHQTQ-------ASVVGLELAA 253 A ++P T A+ AFDL AL +A A G LI+A A + A Sbjct: 200 AWNAVPDTRKPAQAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVT 259 Query: 254 AILCGLFLLKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVSLPFFFHHDLG 313 A+L GL ++ + P+LP+D+F +P FSL+A+T++ SF QG+AFV+LPF F + G Sbjct: 260 ALLAGLAFVRGQRRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYG 319 Query: 314 RSQVEIGLLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTFMPASPSV 373 + E L TPWP+ + ++AP AGR++DR+ +L +GLAL GL+ + +P Sbjct: 320 YTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALFALGLVLLALLPDQAQP 379 Query: 374 WEISWRMLLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALVAL 431 W+I WR L+CGMGFGFFQSPN + ++ + RSG ASG++A R GQ +GAAL+ + Sbjct: 380 WDIGWRALVCGMGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALLGV 437 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 109/344 (31%), Positives = 166/344 (48%), Gaps = 7/344 (2%) Query: 33 ILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMVALLPLAALGEI 92 ++AL I +A LD + N L +A L + VWIV Y L LL AL + Sbjct: 32 LVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADR 91 Query: 93 IGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNTALIRFIYP-TR 151 G RR+Y+ GL LF ASLLC A + P L++AR LQGLG A M + +L+ +Y R Sbjct: 92 FGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDR 151 Query: 152 SLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVLAVPSLPHTSL 211 R +G+ + V +A VGP V ++ W +F +NVP+G++ +V+A +P Sbjct: 152 VRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGG 211 Query: 212 ARHAFDLRSALLNAAAF-GLLILAIGEGAHQTQASVVGLELAAAILCGLFLLKRELSHPA 270 A +L S L GL + I + + + LA A+ G L++ EL A Sbjct: 212 HARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRHELRGIA 271 Query: 271 PMLPVDLFRHPMF-SLSAVTAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVA 329 P+LP LF P F + + + + +F G F L F G ++ GL + P Sbjct: 272 PLLPKALFATPQFAAANGIGFLINFGVYGNLF-YLALFLQQGRGADALQTGLQLVPMMAV 330 Query: 330 VAIMAPIAGRMSDRY--PVGMLGGVGLALLCAGLLSMTFMPASP 371 + ++GRMS + V +L G+ + + L+M F PA+P Sbjct: 331 IFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTA-LAMGFSPATP 373 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 109 bits (272), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 126/432 (29%), Positives = 204/432 (47%), Gaps = 24/432 (5%) Query: 42 LATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMVALLPLAA-LGEIIGYRRIYI 100 + TLDT I NTALPAMA L +V AY L M +L P + L + G RR+Y Sbjct: 64 MQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTM-ASLTPASGWLADRFGTRRMYF 122 Query: 101 WGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLN 160 ++LF+ SLLCA+A SLP L+ AR +QGLGG+ ++ V + P + Sbjct: 123 AAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASI 182 Query: 161 TLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVLAVPSLPHTSLA-RHAFDLR 219 ++ + VGP + ++ W W+F +N+P+GVL + + LP SLA FDL Sbjct: 183 SIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLL 242 Query: 220 SALLNAA---AFGLLILAIGEGAHQTQASVVGLELAAAILCGLFLLKRELSHPAPMLPVD 276 +L +A AF L L + +H+T S+ +AA + G + + HP P+ + Sbjct: 243 GCVLLSACMVAFS-LALDVPFDSHRTGWSIALFVVAALTVLGYW--RHASVHPRPLFRLG 299 Query: 277 LFRHPMFSLS-AVTAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAI--- 332 LF P FS+ A VC + + F+ LG S + G+++ P +A I Sbjct: 300 LFGEPNFSIGLAGNLVCRIGSSAVPFLLP-LLLQVRLGYSPLHAGMVLLPAAIAGTIAKP 358 Query: 333 -MAPIAGRMS-DRYPVGMLGGVGLALLCAGLLSMTFMPASPSVWEISWRMLLCGMGFGFF 390 +AP+ R DR+ + VG A++ L++ + P + ++ +++ M F Sbjct: 359 WIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDW-PLTAAIVQLAIFGASNSMQFAAM 417 Query: 391 QSPNLKALMTSAPPHRSGGASGIVATARLLGQSVGAALVALCFNLSQTQ-GSRLALGFGA 449 S LK L +G + + + ++L +G + NL +++ G+ F Sbjct: 418 NSVTLKGL----DKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATNASFQI 473 Query: 450 AFA--GAACIAS 459 FA GA +AS Sbjct: 474 TFAIVGAITLAS 485 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 96/393 (24%), Positives = 175/393 (44%), Gaps = 6/393 (1%) Query: 42 LATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMVALLPLAALGEIIGYRRIYIW 101 +A LDT IAN ++ +A L + + +++T+Y +A + + L G R++I Sbjct: 44 IAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFIL 103 Query: 102 GLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNTALIRFIYPTRSLGRGVGLNT 161 +V+F S LC +A SL L+ R LQGL G +M ++ L+ I+P VGL Sbjct: 104 SMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWA 163 Query: 162 LIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVLAVPSLP--HTSLARHAFDLR 219 + +A GP + + WP++F +NVP+ ++ + L T +++ D Sbjct: 164 MTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRV 223 Query: 220 SALLNAAAFGLLILAIGEGAHQT---QASVVGLELAAAILCGLFLLKRELSHPAPMLPVD 276 +L L L + EG + +V L + A + FL+ EL+ P++ + Sbjct: 224 GLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLI-WELTERNPIVDLR 282 Query: 277 LFRHPMFSLSAVTAVCSFAAQGLAFVSLPFFFHHDLGRSQVEIGLLMTPWPVAVAIMAPI 336 +FRH F+ S T +F A + V P + +G + G + + + AP+ Sbjct: 283 VFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPM 342 Query: 337 AGRMSDRYPVGMLGGVGLALLCAGLLSMTFMPASPSVWEISWRMLLCGMGFGFFQSPNLK 396 +MS L +G+ L F S ++I W + L G+G F P Sbjct: 343 VAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTG 402 Query: 397 ALMTSAPPHRSGGASGIVATARLLGQSVGAALV 429 ++S + A+G+++ R + ++ ++V Sbjct: 403 LALSSVNEEETASAAGLMSFCRTMAGAIATSIV 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,246,982 Number of extensions: 43850 Number of successful extensions: 359 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 18 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)