BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0462.p (492 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314018|ref|YP_003778110.1| two component sensor histidine ... 987 0.0 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 91 2e-19 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 90 4e-19 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 90 5e-19 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 87 2e-18 >gi|300314018|ref|YP_003778110.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 492 Score = 987 bits (2551), Expect = 0.0, Method: Compositional matrix adjust. Identities = 492/492 (100%), Positives = 492/492 (100%) Query: 1 MLNAIEHHPTPHAAPARPSGYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMSANSR 60 MLNAIEHHPTPHAAPARPSGYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMSANSR Sbjct: 1 MLNAIEHHPTPHAAPARPSGYLGLSFRQLLLAAFLLIAALLSGTSIHALFTLDRMSANSR 60 Query: 61 ETARQAVQLTEAAQRLAERTVAMERSARQYLVLDDPAFHDRFNEARDQARQALGELADSL 120 ETARQAVQLTEAAQRLAERTVAMERSARQYLVLDDPAFHDRFNEARDQARQALGELADSL Sbjct: 61 ETARQAVQLTEAAQRLAERTVAMERSARQYLVLDDPAFHDRFNEARDQARQALGELADSL 120 Query: 121 PKAPREMFGQWSVYVDEAAGVLDADSRKDKGEQARLFQDFARLPALNERIALESRREVDR 180 PKAPREMFGQWSVYVDEAAGVLDADSRKDKGEQARLFQDFARLPALNERIALESRREVDR Sbjct: 121 PKAPREMFGQWSVYVDEAAGVLDADSRKDKGEQARLFQDFARLPALNERIALESRREVDR 180 Query: 181 RNNALSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQP 240 RNNALSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQP Sbjct: 181 RNNALSAALEQQRQMLTAQVVGAIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQP 240 Query: 241 IDVRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVA 300 IDVRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVA Sbjct: 241 IDVRGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVA 300 Query: 301 GQLTDSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQR 360 GQLTDSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQR Sbjct: 301 GQLTDSQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQR 360 Query: 361 LQWLARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRV 420 LQWLARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRV Sbjct: 361 LQWLARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRV 420 Query: 421 ECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREG 480 ECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREG Sbjct: 421 ECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREG 480 Query: 481 GAHFRIELPDEK 492 GAHFRIELPDEK Sbjct: 481 GAHFRIELPDEK 492 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 91.3 bits (225), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 88/325 (27%), Positives = 151/325 (46%), Gaps = 36/325 (11%) Query: 191 QQRQMLTAQVVGAIVLA--VLLAFCFGL-W--LSRPMARLEQAIGRLGDNRFD--QPIDV 243 ++R L +++ ++L ++L L W LSR ++ L + R+ R D PID Sbjct: 62 EKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRARRPDDLSPIDS 121 Query: 244 -RGPADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQ 302 + P +I L + L+ + RL+ A + RF+ +H++KTPLA + + L Sbjct: 122 GQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSEL------AL 175 Query: 303 LTDSQREIARILRQNTQSLQT------QIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVI 356 D Q ++ R L Q +S +T Q+ L R + +Q +PV+L L V+ Sbjct: 176 RQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAE--NQQSQSGGMIPVELNELARSVV 233 Query: 357 DDQRLQWLARELKVEIEGAARTVVV--DPEKMAIVLANLLSNAVRFSPQGG----FIRFL 410 D + + + E +++ +P + +L+NLL NA+ ++P G +R Sbjct: 234 QDWVPMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRAR 293 Query: 411 LSEAPGLVRVECVDQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHS 470 E GL +E D GPG+ + A +FE FY+ L P+ G G+GL++VRE Q H Sbjct: 294 TDEGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTG-SGLGLGLAIVREIAQQHE 352 Query: 471 GKVYLV-------PREGGAHFRIEL 488 + + P+ G FR+ Sbjct: 353 AALEITSNPRSTDPKYPGCVFRVSF 377 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 90.1 bits (222), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 82/288 (28%), Positives = 131/288 (45%), Gaps = 27/288 (9%) Query: 203 AIVLAVLLAFCFGLWLSRPMARLEQAIGRLGDNRFDQPIDVRGPADIRRLGQQLDWLRQR 262 A+VL +A + +P+ + +A +LG N P+ GP ++ Q + +++R Sbjct: 213 AVVLIAWMAVRLAM---KPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRR 269 Query: 263 LADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLTDSQREIARILRQNTQSLQ 322 + D AE++RFL VSH+L++P+ + +L D + R++ + Sbjct: 270 IIDNIAERTRFLAAVSHDLRSPITRMKLRTEMLPDERGKE----------KFRKDLDEMD 319 Query: 323 TQIEDLLRYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLAREL--KVEIEGAARTVV 380 I L + D Q P +DL +LL + DQ E+ +V + G A + Sbjct: 320 ALISATLNVVQ-DIDVQE-RPQLIDLDSLLESLRTDQ------AEVGGEVTLSGHAAPLQ 371 Query: 381 VDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRVECVDQGPGVAPTDAARIFEP 440 P + L NL+ NAVR+ G R L + V + D GPG+ R+ EP Sbjct: 372 GYPRSLRRCLQNLVENAVRY---GQRARVQLQDDADQVCIRVDDDGPGIPEALLERVQEP 428 Query: 441 FYQGLNQPTAARRGNGIGLSVVREYVQAHSGKVYLVPREGGAHFRIEL 488 FY+ A G G+GLS+ VQAH G + LV R GG R+E+ Sbjct: 429 FYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG-LRVEI 475 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 89.7 bits (221), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 80/290 (27%), Positives = 126/290 (43%), Gaps = 10/290 (3%) Query: 189 LEQQRQMLTAQVVGAIVLAVLLAFC---FGLWLSRPMARLEQAIGRLGDNRFDQPIDVRG 245 L Q+ AQ++ A+V +L AF L+RP+ R+ +A RL + ++V Sbjct: 164 LRFQQSQFAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVES 223 Query: 246 PADIRRLGQQLDWLRQRLADLEAEKSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLTD 305 ++ L Q + L L E + F+ VSHEL+TPLA L L+DGV Sbjct: 224 GDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGV----LQ 279 Query: 306 SQREIARILRQNTQSLQTQIEDLLRYNEVSFDAQRIHPVPVDLRALLHK--VIDDQRLQW 363 + R L+ + L + DL + A +DL LL + R Sbjct: 280 PDAQTLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQ 339 Query: 364 LARELKVEIEGAARTVVVDPEKMAIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRVECV 423 L E+ A+ V D ++ +L+NL N++R++ GG ++ + Sbjct: 340 RGMRLSCELP-ASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFD 398 Query: 424 DQGPGVAPTDAARIFEPFYQGLNQPTAARRGNGIGLSVVREYVQAHSGKV 473 D PGV P AR+FE F++ A G G+GL++ R V AH G + Sbjct: 399 DSEPGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTI 448 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 67/224 (29%), Positives = 104/224 (46%), Gaps = 4/224 (1%) Query: 270 KSRFLRHVSHELKTPLAALCEGAALLDDGVAGQLTDSQREIARILRQNTQSLQTQIEDLL 329 KS FL ++SHEL+TPL + A LL D G L+D Q A+ + L I D+L Sbjct: 475 KSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGNDLLALINDIL 534 Query: 330 RYNEVSFDAQRIHPVPVDLRALLHKVIDDQRLQWLAREL--KVEIE-GAARTVVVDPEKM 386 +++ I P L L ++ + + L KVE+E G + + D +++ Sbjct: 535 DLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQRMETDIQRL 594 Query: 387 AIVLANLLSNAVRFSPQGGFIRFLLSEAPGLVRVECVDQGPGVAPTDAARIFEPFYQGLN 446 +L NLLSNA++F+ +G + +A G + D G G+ IFE F Q Sbjct: 595 GQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGEDQHQFIFEAFRQADG 654 Query: 447 QPTAARRGNGIGLSVVREYVQAHSGKVYLVPREG-GAHFRIELP 489 G G+GLS+ R+ G + + R G G+ F + LP Sbjct: 655 STHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,437,449 Number of extensions: 59789 Number of successful extensions: 393 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 338 Number of HSP's successfully gapped: 38 Length of query: 492 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 403 Effective length of database: 1,198,455 Effective search space: 482977365 Effective search space used: 482977365 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)