BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0453.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314014|ref|YP_003778106.1| two component sensor histidine ... 944 0.0 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 286 2e-78 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 175 8e-45 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 169 3e-43 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 169 6e-43 >gi|300314014|ref|YP_003778106.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 944 bits (2439), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/467 (99%), Positives = 466/467 (99%) Query: 1 VAHLFSRRRSVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDL 60 +AHLFSRRRSVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDL Sbjct: 1 MAHLFSRRRSVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDL 60 Query: 61 LRIENGELKASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYP 120 LRIENGELKASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYP Sbjct: 61 LRIENGELKASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYP 120 Query: 121 ALAHIYEDTYNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQA 180 ALAHIYEDTYNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQA Sbjct: 121 ALAHIYEDTYNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQA 180 Query: 181 ALLVVIVSVTLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMA 240 ALLVVIVSVTLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMA Sbjct: 181 ALLVVIVSVTLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMA 240 Query: 241 RLRRLLDHQQRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLAN 300 RLRRLLDHQQRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLAN Sbjct: 241 RLRRLLDHQQRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQEMSGTVERATTLAN 300 Query: 301 QLLSLAKVEQIRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMI 360 QLLSLAKVEQIRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMI Sbjct: 301 QLLSLAKVEQIRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMI 360 Query: 361 GELISNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS 420 GELISNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS Sbjct: 361 GELISNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS 420 Query: 421 SAGGLGLTICGEIVDSMQATISLRNRVGEDGAIEGLNASVHVRVVAG 467 SAGGLGLTICGEIVDSMQATISLRNRVGEDG IEGLNASVHVRVVAG Sbjct: 421 SAGGLGLTICGEIVDSMQATISLRNRVGEDGTIEGLNASVHVRVVAG 467 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 286 bits (733), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 168/456 (36%), Positives = 261/456 (57%), Gaps = 19/456 (4%) Query: 7 RRRSVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENG 66 R S+R+ L+ W++ P+ L + +D+ S+Y N LE + AYDR L+AS ++ + + + +G Sbjct: 7 RLGSLRSQLVRWLLIPLTLLVAVDAVSVYYNALEVADLAYDRSLLASTRALAERVSVVDG 66 Query: 67 ELKASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIY 126 + A +PY AL+ +E D R+ Y++ GL+G+ G DLP + +YPAL Y Sbjct: 67 HVTADVPYVALDSFETDNLGRIYYKVTGLNGELVSGYDDLPPVPPDVKRSEVYPALVRFY 126 Query: 127 EDTYNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVI 186 + Y+ PVR+AAL QPV + RG AL+QV ETME R+ + RKI ++TL RQ L+ V Sbjct: 127 QADYHDQPVRIAALLQPVYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVA 186 Query: 187 VSVTLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLL 246 + + VR L+P+ L+ +++ R DLS + +E++P + A+N M RL+ L+ Sbjct: 187 ALLVWFAVRFVLRPLMQLKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALI 246 Query: 247 DHQQRFVANASHQLRTPLAVLKTQLQSGLR-------GDAPAPVIWQEMSGTVERATTLA 299 Q+RF+A+ASHQLRTPL VLKTQ + LR A + + M+ T + A LA Sbjct: 247 SAQRRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLA 306 Query: 300 NQLLSLAKVEQIRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAE-KILVMGHPW 358 N+LL+LA+ E G L AR+ A++LS +++ E E + + + Sbjct: 307 NRLLTLARAEHGAAEGGTMPLSLSDAARQVALELSQQAVSREIGLAFEEEGDVSIEANAL 366 Query: 359 MIGELISNLLHNAIRHTPAQGRLGIRITARED-----VAELLIWDSGEGIDDQAMENVFK 413 ++ EL+ NLL NAIR+TPA G + +R+ A+ VA L + DSG GI E VF+ Sbjct: 367 LLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFE 426 Query: 414 AF-----SSNVSSAG-GLGLTICGEIVDSMQATISL 443 F + +V+ G GLGL+I EI +A+++L Sbjct: 427 PFYRAGATQHVNPGGTGLGLSIVREIAAMHRASVTL 462 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 175 bits (443), Expect = 8e-45, Method: Compositional matrix adjust. Identities = 130/431 (30%), Positives = 218/431 (50%), Gaps = 16/431 (3%) Query: 22 PIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELKASLPYAALEIYE 81 P+A+++ + + Y N + S DR L+AS I + +G S+P AALE++ Sbjct: 16 PMAVYVGLSTSDAYDNAVSSATLVQDRALLASARMIAGQVGWNDGAPVVSVPPAALELFA 75 Query: 82 ADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIYEDT-YNGVPVRVAAL 140 + + Y++ D G D P+ D A+YP +Y T +NG P+RV + Sbjct: 76 SPAQDAVYYQVLMDDDTLLAGRPDFPA---APDFAALYP----VYSTTSFNGKPLRVVSY 128 Query: 141 FQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSVTLYVVRRALQP 200 + + V V +TM+ R + + +L RQ LL++ +S+ + + L+P Sbjct: 129 IRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVLGLTMELRP 188 Query: 201 VDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQQRFVANASHQL 260 + AL+ +L R D L+P+ +EL+P ++A+NQ + RL + Q+RFVA+A+HQL Sbjct: 189 LLALKDELATRRPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRFVADAAHQL 248 Query: 261 RTPLAVLKTQLQSGLRGDAPAPV--IWQEMSGTVERATTLANQLLSLAKVEQIRGRG-AQ 317 RTPLAV+ +Q+Q + D PA V + + T L N+LL L++ E + Sbjct: 249 RTPLAVIDSQIQFARQLDDPARVAQVLASLQEGSRSMTDLTNKLLLLSQAEASDSSAFPR 308 Query: 318 EVCDLGMLARETAVDLSPLIAEKDLDFELEA--EKILVMGHPWMIGELISNLLHNAIRHT 375 DL ++ +LS L +K +D LE E +V G+ + ++ NL+ NA+R+T Sbjct: 309 APVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAMVNGNEAQLMGMLMNLIDNALRYT 368 Query: 376 PAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS---SAGGLGLTICGE 432 P G++ + + + + + D+G GI + E VF+ F N + GLGL I E Sbjct: 369 PEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFYRNAAPDQPGSGLGLAIVRE 428 Query: 433 IVDSMQATISL 443 IV + Q +I L Sbjct: 429 IVQASQGSIRL 439 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 169 bits (429), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 121/360 (33%), Positives = 187/360 (51%), Gaps = 25/360 (6%) Query: 106 LPSYKGKADKEAIYPALAHIYEDTYNGVPVRVAAL--------FQPVVTNDPRGAALVQV 157 +P D+ + D +G VR+A L Q + +PR A VQV Sbjct: 1 MPQPPPDEDRSTV--GTVQFRNDILHGSDVRIATLQVDLRRSAAQRAASREPR-LATVQV 57 Query: 158 AETMENRSALARKIFWETLIRQAALLVVIVSVTLYVVRRALQPVDALRRQLDERPADDLS 217 ET+E R+ LA +I ++ Q +L V +++ + + R L P+ L++++ R DDLS Sbjct: 58 GETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRARRPDDLS 117 Query: 218 PVAPPMAPRELQPFIDALNQLMARLRRLLDHQQRFVANASHQLRTPLAVLKTQLQSGLRG 277 P+ P E+ P + +LN ++ARL + + Q+RF+A+A+HQ++TPLA ++ Q + LR Sbjct: 118 PIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSELALRQ 177 Query: 278 DAPAPV--IWQEMSGTVERATTLANQLLSLAKVE-QIRGRGAQEVCDLGMLARETAVDLS 334 D V ++++ + E AT L NQLLSLA+ E Q G +L LAR D Sbjct: 178 DDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVVQDWV 237 Query: 335 PLIAEK--DLDFELEAEKILVMGHPWMIGELISNLLHNAIRHTPAQGRLGIRITARED-- 390 P+ + DL FE IL+ G+P M+ EL+SNLL NA+ +TP G + + AR D Sbjct: 238 PMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEG 297 Query: 391 --VAELLIWDSGEGIDDQAMENVFKAF-----SSNVSSAGGLGLTICGEIVDSMQATISL 443 +A L + D+G GI NVF+ F + + S GLGL I EI +A + + Sbjct: 298 GGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEAALEI 357 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 169 bits (427), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 138/451 (30%), Positives = 211/451 (46%), Gaps = 17/451 (3%) Query: 10 SVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELK 69 S+R LL W+I+P+ L +I + Y A+D+ L+ S + +R Sbjct: 7 SIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERTT 66 Query: 70 ASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIYEDT 129 A L A +I +++S + + +G+ GD P ++ + Y+ Sbjct: 67 AELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLPASD----VFDRPIN-YDGE 121 Query: 130 YNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSV 189 G PVR+AA+ Q V N G V VAET+ R I L+ AL + V Sbjct: 122 MRGEPVRIAAM-QVRVKN---GVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFV 177 Query: 190 TLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQ 249 L VRR L+P+ +L+R L++R L + AP ELQP I A+N+LMAR+ + Q Sbjct: 178 VLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQ 237 Query: 250 QRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPVIWQE---MSGTVERATTLANQLLSLA 306 Q F+A+ +HQLRTPL LK QL+ P + M+ +VER + QLL+LA Sbjct: 238 QNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALA 297 Query: 307 KVEQ--IRGRGAQEVCDLGMLARETAVDLSPLIAEKDLDFELEAEKILVMGHPWMIGELI 364 + E R +E+ L L E+ +K +D + + G +++ +LI Sbjct: 298 RSEPGLFESRKLEELS-LDKLVEESVQHFIEEADKKRIDLGFDLRPARLRGDRFLLSDLI 356 Query: 365 SNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAF-SSNVSSAG 423 NL+ NA+R++P G + +R +D L + DSG GI + E +F F +N AG Sbjct: 357 DNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRANDKVAG 416 Query: 424 -GLGLTICGEIVDSMQATISLRNRVGEDGAI 453 GLGL I EI IS+ + G I Sbjct: 417 SGLGLAIVREIAHDHGGVISVDCKEQGQGTI 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,398,912 Number of extensions: 58321 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 289 Number of HSP's successfully gapped: 25 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)