BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0447.p (534 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogen... 1082 0.0 gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogen... 605 e-174 >gi|300314008|ref|YP_003778100.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 1082 bits (2799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 529/534 (99%), Positives = 531/534 (99%) Query: 1 MKARGTEGITYPPQYDVLVVGGGINGAGIARDAAGRGLSVALCEQHDLAQHTSSSSSKLI 60 MKARGTEGITYPPQYDVLVVGGGINGAGIARDAAGRGLSVALCEQHDLAQHTSSSSSKLI Sbjct: 1 MKARGTEGITYPPQYDVLVVGGGINGAGIARDAAGRGLSVALCEQHDLAQHTSSSSSKLI 60 Query: 61 HGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFVMPHDQGQRPAWLIRLGMFLYD 120 HGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFVMPHDQGQRPAWLIRLGMFLYD Sbjct: 61 HGGLRYLEHYEFGLVRKALIEREVLLRSAPHIMWPLRFVMPHDQGQRPAWLIRLGMFLYD 120 Query: 121 HLARRELLPGSRALDLRCHASGAPLKAQFTRGFVYSDGWVDDARLVVLNAMDAAEKGAHI 180 HLARRELLPGSRALDLR HASGAPLKAQFTRGFVYSDGWVDDARLVVLNAMDAAEKGAHI Sbjct: 121 HLARRELLPGSRALDLRRHASGAPLKAQFTRGFVYSDGWVDDARLVVLNAMDAAEKGAHI 180 Query: 181 MTRSRCESLSRQDNGWQAVLRRADGHAMQVRARCVVNAAGPWVAQFLGGAAHVHSSKSVR 240 MTR+RCESL+RQDNGWQAVLRR DGHAMQVRARCVVNAAGPWVAQFLGGAAHVHSSKSVR Sbjct: 181 MTRTRCESLTRQDNGWQAVLRREDGHAMQVRARCVVNAAGPWVAQFLGGAAHVHSSKSVR 240 Query: 241 LVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFTLIGTTDIEYQGRVEDVAISED 300 LVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFTLIGTTDIEYQGRVEDVAIS D Sbjct: 241 LVKGSHIVVPRLFEHDHAYIFQNPDGRIVFAIPYEQDFTLIGTTDIEYQGRVEDVAISAD 300 Query: 301 ETAYLCQLANRYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDLNLQGAPLLS 360 ETAYLCQLANRYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDLNLQGAPLLS Sbjct: 301 ETAYLCQLANRYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDLNLQGAPLLS 360 Query: 361 IFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVRQFDQYVQ 420 IFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVRQFDQYVQ Sbjct: 361 IFGGKITTFRKLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVRQFDQYVQ 420 Query: 421 GLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLLRHEWA 480 GLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLLRHEWA Sbjct: 421 GLQQQYPWLPAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLLRHEWA 480 Query: 481 SNAADILWRRSKLGLHFPKGSEAVLDAWLAARGGQQSGREQAHEALELAQAASR 534 SNAADILWRRSKLGLHFPKGSEAVLDAWLAARGGQQSGREQAHEALELAQAASR Sbjct: 481 SNAADILWRRSKLGLHFPKGSEAVLDAWLAARGGQQSGREQAHEALELAQAASR 534 >gi|300310299|ref|YP_003774391.1| glycerol-3-phosphate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 510 Score = 605 bits (1559), Expect = e-174, Method: Compositional matrix adjust. Identities = 311/502 (61%), Positives = 381/502 (75%), Gaps = 6/502 (1%) Query: 14 QYDVLVVGGGINGAGIARDAAGRGLSVALCEQHDLAQHTSSSSSKLIHGGLRYLEHYEFG 73 +YD+LVVGGGINGAGIARDAAGRGL V LCEQHDLAQHTSS+S+KLIHGGLRYLE+YEF Sbjct: 4 EYDLLVVGGGINGAGIARDAAGRGLRVLLCEQHDLAQHTSSASTKLIHGGLRYLEYYEFK 63 Query: 74 LVRKALIEREVLLRSAPHIMWPLRFVMPHDQGQRPAWLIRLGMFLYDHLARRELLPGSRA 133 LVRKAL EREVLLRSAPHI+WPLRFVMPHD QRPAW+IR G+F+YDHLA+RE+LP S Sbjct: 64 LVRKALQEREVLLRSAPHIIWPLRFVMPHDANQRPAWMIRAGLFMYDHLAKREILPPSEG 123 Query: 134 LDLRCHASGAPLKAQFTRGFVYSDGWVDDARLVVLNAMDAAEKGAHIMTRSRCESLSRQD 193 +L H +G PLK + +GFVYSDGWVDDARLVVLNAMDA E+GA I+TR++C S R+ Sbjct: 124 ANLARHDAGQPLKGDYRKGFVYSDGWVDDARLVVLNAMDAQERGARILTRTKCISARREG 183 Query: 194 NGWQAVL---RRADGHAMQVRARCVVNAAGPWVAQFLGGAAHVHSSKSVRLVKGSHIVVP 250 + W AVL R +VRAR +VNAAGPW A+F S +S+RL+KGSHIVV Sbjct: 184 DIWHAVLEHQREGGVQQEEVRARAIVNAAGPWAARFAQAVPQAKSGRSLRLIKGSHIVVK 243 Query: 251 RLFEHDHAYIFQNPDGRIVFAIPYEQDFTLIGTTDIEYQGRVEDVAISEDETAYLCQLAN 310 R+F+H +AYIFQNPD RI+FAIPY+++FTLIGTTD+EY G V I E YLC +AN Sbjct: 244 RMFDHPYAYIFQNPDKRIIFAIPYQEEFTLIGTTDLEYHGDAGKVEIDAAEIDYLCDMAN 303 Query: 311 RYFEKPITPDDVVWSYSGVRPLLEDAAADASAVTRDYQLDLNLQGAPLLSIFGGKITTFR 370 RYF++ I +DVVW+YSGVRPLL+D+A +ASA+TRDY L+ + GAP+L+++GGKITT+R Sbjct: 304 RYFKRQIGKEDVVWTYSGVRPLLDDSAENASAITRDYLLECDDAGAPMLNVWGGKITTYR 363 Query: 371 KLAEEAVDMIAPVLGNQRGAWTEHGCLPGGDLYGAQPSNRSVR-QFDQYVQGLQQQYPWL 429 KLAEEA+ M+AP LG +WT LPGGDL QP R FD Y++ Q ++PWL Sbjct: 364 KLAEEALAMLAPHLGASSQSWTAGAPLPGGDL--EQPGKMVARYDFDAYLRKFQARHPWL 421 Query: 430 PAALLQRYARAYGSRTHTLLEGRQNVEEMGEEILDGLYAAEVDYLLRHEWASNAADILWR 489 PA L RYAR+YGSR ++ ++ +GEE+ GLY AE YL++HEWA +A DILWR Sbjct: 422 PAELAHRYARSYGSRVEQIIGQADSLAALGEELSPGLYEAEARYLVQHEWARSADDILWR 481 Query: 490 RSKLGLHFPKGSEAVLDAWLAA 511 RSKLGLH L+AWLA+ Sbjct: 482 RSKLGLHCGPQHRGRLEAWLAS 503 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,702,490 Number of extensions: 72579 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 2 Length of query: 534 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 444 Effective length of database: 1,193,720 Effective search space: 530011680 Effective search space used: 530011680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)