BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0434.p (417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313995|ref|YP_003778087.1| urea ABC transporter periplasmi... 854 0.0 gi|300311947|ref|YP_003776039.1| branched-chain amino acid ABC t... 277 1e-75 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 65 9e-12 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 59 1e-09 gi|300312955|ref|YP_003777047.1| amino acid ABC transporter peri... 56 4e-09 >gi|300313995|ref|YP_003778087.1| urea ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 854 bits (2206), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/417 (99%), Positives = 415/417 (99%) Query: 1 MSRRTVLKATALGAFALAASSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTID 60 MSRRTVLKATALGAFALAASSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTID Sbjct: 1 MSRRTVLKATALGAFALAASSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTID 60 Query: 61 EINAAGGVMGKKLEPVVVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK 120 EINAAGGVMGKKLEPVVVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK Sbjct: 61 EINAAGGVMGKKLEPVVVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVFK 120 Query: 121 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFVLLGTDYVYP 180 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFVLLGTDYVYP Sbjct: 121 ELNSLLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFVLLGTDYVYP 180 Query: 181 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFSAGGKTAVISTINGDSN 240 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFSAGGKTAVISTINGDSN Sbjct: 181 RTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFSAGGKTAVISTINGDSN 240 Query: 241 VPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFESVKNQVNAAFIK 300 VPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFESVKN VN AFIK Sbjct: 241 VPFYKELGNAGLKATDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFESVKNPVNTAFIK 300 Query: 301 KWKEYAKAKNLPNADTVVTNDPMEATYVGIHMWAQAVEKAKSTDTDKVIAAMGGQTYKAP 360 KWKEYAKAKNLPNADTVVTNDPMEATYVGIHMWAQAVEKAKSTDTDKVIAAMGGQTYKAP Sbjct: 301 KWKEYAKAKNLPNADTVVTNDPMEATYVGIHMWAQAVEKAKSTDTDKVIAAMGGQTYKAP 360 Query: 361 SGYTLEMDKTNHHLHKPVMIGEIKADGQFNVVYKTKTTIRAQPWSPYIPGNESKQKN 417 SGYTLEMDKTNHHLHKPVMIGEIKADGQFNVVYKTKTTIRAQPWSPYIPGNESKQKN Sbjct: 361 SGYTLEMDKTNHHLHKPVMIGEIKADGQFNVVYKTKTTIRAQPWSPYIPGNESKQKN 417 >gi|300311947|ref|YP_003776039.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 277 bits (709), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 156/366 (42%), Positives = 225/366 (61%), Gaps = 16/366 (4%) Query: 32 IKVGILHSLSGTMAISETSLKDVALMTIDEINAAGGVMGKKLEPVVVDPASNWPLFAEKA 91 + VG LHS +GTMAISET + ID+INA GG++G+K++ + D AS+WP FAEKA Sbjct: 56 VTVGQLHSATGTMAISETGSIQAERLAIDQINAMGGILGRKIKIIQEDGASDWPTFAEKA 115 Query: 92 RQLISQDKVSVVFGCWTSVSRKSVLPVFKELNSLLFYPVQYEGEELEKNVFYTGAAPNQQ 151 ++L+ D+V+ VFGCWTS SRK+VLPVF++ N LL+YP YEG E KNVFYTG QQ Sbjct: 116 KKLLENDRVAAVFGCWTSASRKAVLPVFEKDNGLLYYPTFYEGLEQSKNVFYTGQEATQQ 175 Query: 152 AIPAVEYLMSKEGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVKDSDIDEVYTPFGHS 211 + + ++ ++ AK F L+G+DY++PRT+NKI R + + +K S + E Y P G++ Sbjct: 176 VLAGLNWVAKEK--KAKTFFLVGSDYIWPRTSNKIARKHIENV-LKGSVVGEEYYPLGNT 232 Query: 212 DYQTIVANIKKFSAGGKTAVISTINGDSNVPFYKELGNAGLKATDVPVVAFSVGEEELRG 271 + +++ IK + + + G SNV FYK+L AG+ A ++ SV E+E+ G Sbjct: 233 QFGSLINKIK---LKKPDVIFADVVGGSNVAFYKQLKAAGVTAKTQNLLTISVTEDEMLG 289 Query: 272 VDTKPLVGHLAAWNYFESVKNQVNAAFIKKWKEYAKAKNLPNADTVVTNDPMEATYVGIH 331 + + + G A+ YF+S+ N N F+ ++ KAK N+ V D +A Y+G Sbjct: 290 IGGENVEGFFASMKYFQSLDNANNKKFVAEF----KAKFGANS---VIGDVTQAAYLGPW 342 Query: 332 MWAQAVEKAKSTDTDKVIAAMGGQTYK-APSGYTLEMDKTNHHLHKPVMIGEIKADGQFN 390 +W AVEKA S D DKV+AA G K AP GY + NHHL IGE + DGQF Sbjct: 343 LWKAAVEKAGSFDVDKVVAASPGIELKTAPEGYVKVHE--NHHLWSKTRIGEAQKDGQFK 400 Query: 391 VVYKTK 396 VVY+++ Sbjct: 401 VVYESE 406 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 65.1 bits (157), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 101/399 (25%), Positives = 171/399 (42%), Gaps = 58/399 (14%) Query: 14 AFALAA-SSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTIDEINAAGGVMGKK 72 AF AA S ++ AAD IK+G+ +G A S++D + + EINAAGG++G++ Sbjct: 7 AFVAAALMSLALTSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGRQ 66 Query: 73 LEPVVVDPASNWPLFAEKARQLISQDKVSVVFG-CWTSVS-------RKSVLPVFKELNS 124 ++ + D + + A++LI+++KV G T V+ +++ +PV + + Sbjct: 67 IQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVAT 126 Query: 125 LLFYPVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFV---LLGTDYVYPR 181 Q+E E+ + VF T AA QA +++ E ++F +L Y + Sbjct: 127 GTVITKQFEKEK-DNYVFRTSAADKIQA-----KMIADEAVDKQKFTKVAILADSTNYGQ 180 Query: 182 TTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQT----IVANIKKFSAGGKTAVISTING 237 + L L K V TP Y + A + K GG AV++ G Sbjct: 181 LGREDLEKVLADKKV---------TPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIG 231 Query: 238 DSNVPFYKELGNAGLKAT-DVPVVA---FSVGEEELRGVDTKPLVGHLAAW--NYFESVK 291 P ++ N K VP++ S+G +D G+ A + + Sbjct: 232 ----PELAQIANGMEKLNWHVPIIGSWPLSMGNF----IDNSGKNGNGARMPQTFIQDGN 283 Query: 292 NQVNAAFIKKWKEYAKAKNLPNADTVVTNDPMEATYVGIHMWAQAVEKAKSTDTDKVIAA 351 AFI+ +++ K +P+A + Y I + A A+++A +TD DKV AA Sbjct: 284 TPKRKAFIEAYQKAYKVDRMPSAVSAAQG------YDSILLLAAAIKQAGTTDGDKVRAA 337 Query: 352 MGGQTYKAPSGYTLEMDK----TNHHLHKPVMI--GEIK 384 + K G DK +H KP M+ GE+K Sbjct: 338 LENLNTKV-EGVVTTYDKPFTHDDHEAIKPAMVVMGEVK 375 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 90/353 (25%), Positives = 146/353 (41%), Gaps = 41/353 (11%) Query: 29 ADTIKVGILHSLSGTMAISETSLKDVALMTIDEINAAGGVMGKKLEPVVVDPASNWPLFA 88 A+T+K+ I ++G +A +K AL I++INAAGG G K E V++D A Sbjct: 26 AETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAV 85 Query: 89 EKARQLISQDKVSVVFGCWTSVSRKSVLPVFKELNSLLFYPV----QYEGEELEKNVFYT 144 A +++SQ + V G S S +++ ++ P Q + K +F T Sbjct: 86 AVANKIVSQG-IKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRT 144 Query: 145 GAAPNQQAIPAVEYLMSKEGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVKDSDIDEV 204 +QQ A Y++ K K+ +L Y + ++A L + V + + Sbjct: 145 IGRDDQQGPAAARYVIEKL--KPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFEGI 202 Query: 205 YTPFGHSDYQTIVANIKKFSA-----GGKTAVISTINGDSNVPFYKELGNAGLKATDVPV 259 G SDY IV +K GG ++ E+G +A + + Sbjct: 203 NA--GDSDYSAIVTKLKSQGVDFVYFGG---------------YHPEMGLIMRQAREQGI 245 Query: 260 VAFSVGEEELRGVDTKPLVGHLAAWNYFESVKNQVNA--AFIKKWKEYAKAKNLPNADTV 317 A +G E + D + G A+ ++ A A K +A AK PN Sbjct: 246 KAVFMGPEGVGNKDITAIAGP-ASEGMLVTLPADFAADPANAALVKAFAAAKRDPNG--- 301 Query: 318 VTNDPME-ATYVGIHMWAQAVEKAKSTDTDKVIAAMGGQTYKAPSGYTLEMDK 369 P + Y G+ + A A+ AKSTD +KV A + ++K P G +E DK Sbjct: 302 ----PFQMPAYSGVKIIADAIAGAKSTDPEKVAAYIHANSFKTPIG-NVEYDK 349 >gi|300312955|ref|YP_003777047.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 392 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 79/314 (25%), Positives = 139/314 (44%), Gaps = 26/314 (8%) Query: 1 MSRRTVLKATALGAFALAASSWLPSAFAADTIKVGILHSLSGTMAISETSLKDVALMTID 60 M+R + + + A A+ AS LP A AAD +KVG L +LSG + ++ ++ + Sbjct: 1 MNRLSKVAGAVMMAMAVGAS--LP-AQAADVVKVGFLTTLSGPASAPGVDEREGFMLALK 57 Query: 61 EI-NAAGGVMGKKLEPVVVDPASNWPLFAEKARQLISQDKVSVVFGCWTSVSRKSVLPVF 119 + N GGV E +VVD + + A +LI ++KV ++ G S +V P Sbjct: 58 TLGNKLGGV---PAEVIVVDDQFSPDAAKQGADRLIKREKVDILSGFVYSNILLAVAPAA 114 Query: 120 KELNSLLFY----PVQYEGEELEKNVFYTGAAPNQQAIPAVEYLMSKEGGGAKRFVLLGT 175 +++ P Q G F++ + N A A LM+ + G +R ++ Sbjct: 115 FASKTVVVSANAGPAQLAGAGCNP-YFFSASYQNDAAHEAAGKLMADK--GYQRVAIIAP 171 Query: 176 DYVYPRTTNKILRAFLHSKGVKDSDIDEVYTPFGHSDYQTIVANIKKFSAGGKTAVISTI 235 +Y + + + FL KD + E++ G D+ T +A ++ A A+ + Sbjct: 172 NYPAGKDASAGFKRFLK----KDPAV-ELFPKLGQLDFATEIAQLR---AANIDALYFFL 223 Query: 236 NGDSNVPFYKELGNAGLKATDVPVV-AFSVGEEELRGVDTKPLVGHLAAWNYFESVKNQV 294 G V F K+ AG+ V V+ A+ ++ L D V + W+ + N+ Sbjct: 224 PGGMGVNFVKQYAAAGMDPKIVRVMPAYDADQQMLSNGDLIAGVANTGQWS--SDLPNEQ 281 Query: 295 NAAFIKKW-KEYAK 307 N AF+ + KEY + Sbjct: 282 NRAFVAAYLKEYGR 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,258,746 Number of extensions: 51718 Number of successful extensions: 161 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 7 Length of query: 417 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 329 Effective length of database: 1,203,190 Effective search space: 395849510 Effective search space used: 395849510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)