BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0429.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 801 0.0 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 116 4e-27 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 89 7e-19 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 65 1e-11 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 62 8e-11 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 801 bits (2069), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/406 (99%), Positives = 405/406 (99%) Query: 1 MQNNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFL 60 MQNNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFL Sbjct: 1 MQNNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFL 60 Query: 61 IGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAG 120 IGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAG Sbjct: 61 IGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAG 120 Query: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGL 180 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGL Sbjct: 121 MVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGL 180 Query: 181 LCILGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAG 240 LCILGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAG Sbjct: 181 LCILGTRKYLTETVDVSRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAG 240 Query: 241 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVLGKVLWVPAGLSLT 300 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVLGKVLWVPAGLSLT Sbjct: 241 GPFVVIELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVLGKVLWVPAGLSLT 300 Query: 301 VALLVLAGLETLPLLLLGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGSLQFGMG 360 V+LLVL GLETLPLLLLGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGSLQFGMG Sbjct: 301 VSLLVLVGLETLPLLLLGFFGFLTAYGFTGPNATAIALKHQGRQAGTAAALMGSLQFGMG 360 Query: 361 VLSGVAMSLWHDGTALPLVSVMAVCGVTALLVYHFAAKHAPAVSPQ 406 VLSGVAMSLWHDGTALPLVSVMAVCGVTALLVYHFAAKHAPAVSPQ Sbjct: 361 VLSGVAMSLWHDGTALPLVSVMAVCGVTALLVYHFAAKHAPAVSPQ 406 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 116 bits (290), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 90/311 (28%), Positives = 151/311 (48%), Gaps = 13/311 (4%) Query: 17 LAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDR 76 L ++ + G LAID+Y+P+ +A D +Q ++ +LI A GQ+ +GPL+D Sbjct: 23 LFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLADA 82 Query: 77 FGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 +GRK L G+ L+ + +L + A N+ET + R+ QG G +A ++ +A++ D + Sbjct: 83 YGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQA 142 Query: 137 SAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVDV 196 A++ + + +GLAPI+AP IG + WR V L ++ L +Y Sbjct: 143 LVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYRETLAQP 202 Query: 197 SRAEPLHLGRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVVIELHGVKPQYF 256 R P L R R +L F S L GL + + + GPF+V P +F Sbjct: 203 VRLHPATLARNARM---VLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPPTFF 259 Query: 257 GFVFASNAIGL------IAASQVNARLVVKRSAERVLGKVLWVPAGLSLTVALLVLAGLE 310 G++ + AIG+ I+ + RL R+ +V+ A + L +AL V + Sbjct: 260 GYL--ALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALSVGLTVW 317 Query: 311 TL--PLLLLGF 319 TL P+L++ F Sbjct: 318 TLCAPVLVMAF 328 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 88.6 bits (218), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 59/202 (29%), Positives = 99/202 (49%), Gaps = 1/202 (0%) Query: 3 NNDTSLSTGKFPAWLAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIG 62 ND SL+ + ++A M L + + +AQ GVG N++ L + +++ Sbjct: 19 TNDNSLTDARVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLT 78 Query: 63 VAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMV 122 +AV G ++DRFG + IA++ + S+LC F+QN+ R+ QGLGG+ + Sbjct: 79 LAVFIPISGWVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVP 138 Query: 123 IPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLC 182 + R +V G + KA + + +AP++ P IGG + +ASW IF++ FGL Sbjct: 139 VGRMIVVRSTGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAA 198 Query: 183 ILGTRKYLTETVDVSRAEPLHL 204 + VS+ PL L Sbjct: 199 FTAVMMVVRNEHGVSK-RPLDL 219 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 46/174 (26%), Positives = 89/174 (51%), Gaps = 4/174 (2%) Query: 23 LTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDRFGRKPP 82 +T + + + LP+ +A+DLG +Q++ ++++++ A + G +DR+GR+ Sbjct: 47 MTVLDTNVVGIILPT---VARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRV 103 Query: 83 LFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGS-AKAF 141 L GIAL+ + SL C A + + L R QG+G + + A++ E +A+ Sbjct: 104 LLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDEDQRERAW 163 Query: 142 SMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVD 195 ++ + GL +LAP IGG++ WR F V LL + +++ E+ + Sbjct: 164 ALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRN 217 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 62.0 bits (149), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 42/168 (25%), Positives = 80/168 (47%), Gaps = 1/168 (0%) Query: 37 SFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDRFGRKPPLFFGIALYFVCSLL 96 S A+A D+ V ++L L ++L+ +AV G ++DRFG I ++ + S+L Sbjct: 9 SLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAICVFMLGSIL 68 Query: 97 CIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEGSAKAFSMLMLVFGLAPILAP 156 C + + + R QG+GG+ + + R ++ + KA S L + P+L P Sbjct: 69 CGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGP 128 Query: 157 FIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLTETVDVSRAEPLHL 204 +GG + + WR IF + +L + +++ + + + PL L Sbjct: 129 PLGGFITTYFHWRWIFFINIPISILGLYLATRFIPD-LKAEQVAPLDL 175 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,186,559 Number of extensions: 49439 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 307 Number of HSP's successfully gapped: 25 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)