BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0424.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid li... 1127 0.0 gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA lig... 224 1e-59 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 160 3e-40 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 133 4e-32 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 130 3e-31 >gi|300313986|ref|YP_003778078.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 1127 bits (2916), Expect = 0.0, Method: Compositional matrix adjust. Identities = 545/546 (99%), Positives = 546/546 (100%) Query: 1 MADFDTGLGRNAANYAALTPIDFIARAAAVYGKRTAIIHGALRQDWDQTYRRTRRLASAL 60 MADFDTGLGRNAANYAALTPIDFIARAAAVYGKRTAIIHGALRQDWDQTYRRTRRLASAL Sbjct: 1 MADFDTGLGRNAANYAALTPIDFIARAAAVYGKRTAIIHGALRQDWDQTYRRTRRLASAL 60 Query: 61 QGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL 120 QGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL Sbjct: 61 QGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAESIVFMLRHGEAKVLL 120 Query: 121 IDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPA 180 IDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPA Sbjct: 121 IDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPA 180 Query: 181 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC 240 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC Sbjct: 181 DEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC 240 Query: 241 FPWTVAARAGVNVCLRKFEPKLVFDLIAEHGVTHYCAAPIVHAALANAPEGWRAGIRGPV 300 FPWTVAARAGVNVCLRKFEPKLVFDLIAEHG+THYCAAPIVHAALANAPEGWRAGIRGPV Sbjct: 241 FPWTVAARAGVNVCLRKFEPKLVFDLIAEHGITHYCAAPIVHAALANAPEGWRAGIRGPV 300 Query: 301 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSR 360 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSR Sbjct: 301 KAMVAGAPPPAAVLAKMEAMQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSR 360 Query: 361 QGVRYHLQSGVTVLSPDTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFH 420 QGVRYHLQSGVTVLSPDTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFH Sbjct: 361 QGVRYHLQSGVTVLSPDTMAPVAADGEEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFH 420 Query: 421 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE 480 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE Sbjct: 421 TGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGE 480 Query: 481 TPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS 540 TPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS Sbjct: 481 TPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKAIYFGPLPKTSTGKIQKFELRKRMKS 540 Query: 541 DSAINV 546 DSAINV Sbjct: 541 DSAINV 546 >gi|300310928|ref|YP_003775020.1| medium-chain-fatty-acid CoA ligase [Herbaspirillum seropedicae SmR1] Length = 545 Score = 224 bits (572), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 176/521 (33%), Positives = 264/521 (50%), Gaps = 27/521 (5%) Query: 37 IIHGALRQDWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLN 96 II+G R + Y R +RLA+AL+ +G+ +TV+ M ++ +E F VPM G VL Sbjct: 34 IIYGQRRLSYAGFYERVQRLANALKEMGLQPGNTVAVMDWDSNRYLECFFAVPMMGCVLQ 93 Query: 97 ALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPAL-KIVEVFDELGPPPVAG 155 +N+RL E I + L H +A VLL++S+F L +Q+R +L L + V + D+ P P G Sbjct: 94 TVNVRLSPEQIAYTLNHAQADVLLVNSDFLPLLKQIRGELTTLTRCVWLTDDATPCP-RG 152 Query: 156 ERFGHLEYEALLAGGDEHFDWQMPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSN 215 F EYE LLA + D+ DE YT+GTTG PKGV + HR L+ ++ Sbjct: 153 PGFAG-EYEELLAQAEPACDFP-DFDENARATTFYTTGTTGLPKGVYFSHRQLVLHTLAT 210 Query: 216 ILEWDLP-------KHPVYLWTLPMFHCNGWCFPWTVAARAGV-NVCLRKFEPKLVFDLI 267 L + VY+ PMFH + W P+ VA AGV V ++ P + LI Sbjct: 211 TAGLALAPQQGRFHRDDVYMPLTPMFHVHAWGLPY-VATMAGVKQVYPGRYVPDTICRLI 269 Query: 268 AEHGVTHYCAAPIVHAALANAPEGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQ-FELTH 326 E VT P + + + + A + G M+ G LAKM + ++ Sbjct: 270 REEKVTFSHCVPTILQMVLTSEQAREADLSG--WKMIIGGSAMTGSLAKMARDRGIDVFT 327 Query: 327 VYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMAPVAADG 386 YG++E P A Q L ++ A + + G L TV M DG Sbjct: 328 GYGMSETC-PVLTLA-QVPTEELGGEREAAIRVKTGRPLPLVDLRTV--DGEMQDCPRDG 383 Query: 387 EEIGEIMFRGNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISG 446 GE++ R +GYL A+ E + GGW HT D+G ++A+GY+++ DR KD+I +G Sbjct: 384 STTGEVVVRAPWLTQGYLHQAEASEELWRGGWLHTQDIGHLDAEGYLQVTDRIKDVIKTG 443 Query: 447 GENISSVEVEDVLYRHPAVLAAAVVAQPDEKWGETPCAFVEL-KEGAEVGAAELTEFCRS 505 GE +SS+++ED++ RHPAV AV+ D KWGE P A V L K+ A V ++ E R+ Sbjct: 444 GEWVSSLQIEDIIARHPAVAETAVIGIQDGKWGERPVALVVLKKDAAAVSEDDIREHVRA 503 Query: 506 -----HLAGFKVPKAIYF-GPLPKTSTGKIQKFELRKRMKS 540 ++ + VP + F L +TS GK+ K +R++ S Sbjct: 504 AAEAGAISRYGVPDRVRFVAELARTSVGKLNKRVMREQYPS 544 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 160 bits (404), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 143/501 (28%), Positives = 214/501 (42%), Gaps = 31/501 (6%) Query: 46 WDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGVPMAGAVLNALNIRLDAE 105 W + R LA AL GV K D + N + E+ + G V N R+ Sbjct: 65 WREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPP 124 Query: 106 SIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIVEVFDELGPPPVAGERFGHLEYEA 165 ++ + A V+L D FA ++ PAL+ V +A R G L+YEA Sbjct: 125 EAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVA--------LADARAGELDYEA 176 Query: 166 LLAGGDEHFDWQMPADEWDAIALNYTSGTTGDPKGVVYHHRGAALNAVSNILEW--DLPK 223 L+ G + D D + YTSGTTG PK + H A +++ + L Sbjct: 177 LVREGSGAAFREAEVDYDDPLWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLTH 236 Query: 224 HPVYLWTLPMFHCNGWCFPWTVAARAGVNVCL--RKFEPKLVFDLIAEHGVTHYCAAPIV 281 L P+ H G AR ++ L + + + L+ ++ V + P + Sbjct: 237 QSRSLVVAPLSHGAG-VHAVINTARGAASILLSSERLVVEEAWQLVEKYRVDNLFTVPTI 295 Query: 282 HAALANAPEGWRAGIRGPVKAMVAGAPP-PAAVLAKMEAMQFELTHVYGLTEVYGPAAVC 340 L R + AGAP A + ++ + L YGL EV G Sbjct: 296 VKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFL 355 Query: 341 AEQDEWNTLSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMAPVAADGEEI-----GEIMFR 395 L + A ++R G ++G+ + A + DG+E+ GEI R Sbjct: 356 PPY-----LHFEDDAHPQARVGTCGMPRTGMQI------AILNDDGQELAPFETGEICVR 404 Query: 396 GNICMKGYLKNDRATHEAFAGGWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEV 455 G GY N A +AF WFHTGDLG ++ DG++ I RS D+ ISGG N+ E+ Sbjct: 405 GPAVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREI 464 Query: 456 EDVLYRHPAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKA 515 E+ L HPAV AV+ PD KWGE+ A + K G +V L S + +K P+ Sbjct: 465 EEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLAHLESRIGKYKWPRR 524 Query: 516 -IYFGPLPKTSTGKIQKFELR 535 IY+ +PK+ GKI K +++ Sbjct: 525 FIYWDSMPKSGYGKIVKKQIK 545 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 133 bits (334), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 119/374 (31%), Positives = 173/374 (46%), Gaps = 47/374 (12%) Query: 184 DAIALNYTSGTTGDPKGVVYHHRGAALNAVSNIL---EWDLP--------KHPVYLWTLP 232 D L YT GTTG KG V HR N V+N+L EW P V++ LP Sbjct: 206 DIAFLQYTGGTTGVAKGAVLTHR----NLVANVLQAEEWLTPALKAGKPIDQLVFVCALP 261 Query: 233 MFHCNGWCFPWTVAARAG-VNVCLRKFE--PKLVFDLIAEHGVTHYCAAPIVHAALANAP 289 ++H + R G +N+ + P V +L A++ V + A ++ AL N Sbjct: 262 LYHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKEL-AKYKVNTFPAVNTLYNALLNNA 320 Query: 290 EGWRAGIRGPVKAMVAGAPPPAAVLAKMEAMQ-FELTHVYGLTEVYGPAAVCAEQDEWNT 348 + R G + G +V K + + + YGL+E A+ N Sbjct: 321 DFARLDFSGYRCCVGGGMAVQKSVADKWKKLTGCPIIEGYGLSETSPIASA-------NP 373 Query: 349 LSVDQRAVQKSRQGVRYHLQSGVTVLSPDTMAPVAADGEEI-----GEIMFRGNICMKGY 403 ++D+ Y G+ + S + A + DG+ + GEI RG M GY Sbjct: 374 CTIDE-----------YTGTIGLPLPSTE-FAILDDDGQPVPLGQPGEIAIRGPQVMPGY 421 Query: 404 LKNDRATHEAFA-GGWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRH 462 + T + G+F TGD+GVM+ GY KI DR KD+I+ G N+ EVE V+ +H Sbjct: 422 WQRPDETAKVMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYPNEVEGVVAQH 481 Query: 463 PAVLAAAVVAQPDEKWGETPCAFVELKEGAEVGAAELTEFCRSHLAGFKVPKAIYF-GPL 521 P VL A V PDE GE FV +++ + AA+L ++C+ G+K PK I F L Sbjct: 482 PGVLECACVGVPDEHTGEAVKLFV-VRKDKTLSAAQLMDYCKEQFTGYKKPKYIEFRDEL 540 Query: 522 PKTSTGKIQKFELR 535 PKT+ GKI + ELR Sbjct: 541 PKTNVGKILRRELR 554 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 130 bits (327), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 153/544 (28%), Positives = 212/544 (38%), Gaps = 86/544 (15%) Query: 34 RTAII----HGALRQ-DWDQTYRRTRRLASALQGLGVGKNDTVSAMLPNTPAMVEAHFGV 88 RTAII G++ +DQ + R A+AL+ GVG+ D + L V H Sbjct: 48 RTAIICEDADGSVTSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAA 107 Query: 89 PMAGAVLNALNIRLDAESIVFMLRHGEAKVLLIDSEFAALAQQLRTQLPALKIV------ 142 GA+ L ++I + L + A L+ D+ A L +LPAL++V Sbjct: 108 YKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDASGMEKAT-LAGELPALRLVFCVESS 166 Query: 143 --------EVFDELGPPPVAGERFGHLEYEALLAGGDEHFDWQMPADEWDAIALNYTSGT 194 + +D L P E + +L D D + YTSGT Sbjct: 167 DLVLPQTTDFWDRLHAAPA--------ELDPVLTLAD------------DPAMIIYTSGT 206 Query: 195 TGDPKGVVYHHRGAALNAVSNILEWDLPKHPVYLWTLPMFHCNGWC-------------- 240 TG KG ++ HR +L LP V + P W Sbjct: 207 TGKAKGALHAHR---------VLLGHLPGVEVSHDSFPQEGDRFWTPADWAWIGGLLDVL 257 Query: 241 FPWTVAARAGVNVCLRKFEPKLVFDLIAEHGVTHYCAAPIVHAALANAPEGWRAGIRGPV 300 P A V L KF+ VF L+A H + + P L A RA + Sbjct: 258 LPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAAT-VRAQADFSL 316 Query: 301 KAMVAGAPPPAAVLAK--MEAMQFELTHVYGLTEVYGPAAVCAEQDEWNTLSVDQRAVQK 358 +++ +G L EA+ + YG Q E N + Sbjct: 317 RSVASGGETLGDDLIAWGREALGVTINEFYG-------------QTECNLVVSSSSHCYP 363 Query: 359 SRQGVRYHLQSGVTVLSPDTMAPVAADGEEIGEIMFRGN--ICMKGYLKNDRATHEAFAG 416 S G G V D V G +G I R + Y +N+ AT E FAG Sbjct: 364 SVSGSMGRAVPGHVVQIVDEQGQVLPHGT-VGNIAIRAPDPVMFLRYWRNEEATREKFAG 422 Query: 417 GWFHTGDLGVMNADGYIKIKDRSKDIIISGGENISSVEVEDVLYRHPAVLAAAVVAQPDE 476 + TGDLG M+ GYI+ R+ D+I S G I +E+ L RHPAV AAVV D Sbjct: 423 DFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDA 482 Query: 477 KWGETPCAFVELKEGA---EVGAAELTEFCRSHLAGFKVPKAIYF-GPLPKTSTGKIQKF 532 E AFV LK+G E AEL + R+ LA + P+ I F LP T+TGKI + Sbjct: 483 LRTEVVKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRK 542 Query: 533 ELRK 536 L++ Sbjct: 543 TLKE 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,715,720 Number of extensions: 72644 Number of successful extensions: 267 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 14 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)