BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0421.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotrans... 729 0.0 gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotrans... 138 7e-34 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 70 2e-13 >gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 360 Score = 729 bits (1881), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/360 (99%), Positives = 359/360 (99%) Query: 1 MSKFWSPIVSRLTPYTPGEQPKIARLVKLNTNESPYGPSPLALQAIAREVSDSLRLYPNP 60 MSKFWSPIVSRLTPYTPGEQPKIARLVKLNTNESPYGPSPLALQAIAREVSDSLRLYPNP Sbjct: 1 MSKFWSPIVSRLTPYTPGEQPKIARLVKLNTNESPYGPSPLALQAIAREVSDSLRLYPNP 60 Query: 61 DAEPLKQAIARRHAADGVSVREVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTY 120 DAEPLKQAIARRHAADGVSVREVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTY Sbjct: 61 DAEPLKQAIARRHAADGVSVREVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTY 120 Query: 121 AGLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGIIFPNPNAPTGCLLGLADVERILQGNP 180 AGLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGIIFPNPNAPTGCLLGLADVERILQGNP Sbjct: 121 AGLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGIIFPNPNAPTGCLLGLADVERILQGNP 180 Query: 181 ERVVVVDEAYIDFGGHSAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLIDALER 240 ERVVVVDEAYIDFGG SAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLIDALER Sbjct: 181 ERVVVVDEAYIDFGGQSAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLIDALER 240 Query: 241 VKNSFNSYPLDRPAIAGATAAIEDEDYFRKTCDMVIASRTALAAQLAGLGFQVLPSAANF 300 VKNSFNSYPLDRPAIAGATAAIEDEDYFRKTC+MVIASRTALAAQLAGLGFQVLPSAANF Sbjct: 241 VKNSFNSYPLDRPAIAGATAAIEDEDYFRKTCNMVIASRTALAAQLAGLGFQVLPSAANF 300 Query: 301 LFVRHPQHDAAQLAAALRADGIIVRHFKSERISQFLRITIGTDEDCGVLVEALRRHLGQQ 360 LFVRHPQHDAAQLAAALRADGIIVRHFKSERISQFLRITIGTDEDCGVLVEALRRHLGQQ Sbjct: 301 LFVRHPQHDAAQLAAALRADGIIVRHFKSERISQFLRITIGTDEDCGVLVEALRRHLGQQ 360 >gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 138 bits (347), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 124/364 (34%), Positives = 189/364 (51%), Gaps = 25/364 (6%) Query: 8 IVSRLTPYTPGEQ----PKIARLVKLNTNESPYG-PSPLALQAIAREVSDSLRLYPNPDA 62 I + + P G Q P +VKL+ E+PY P+ L Q R + L YP P Sbjct: 17 IANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLAQRLAALELNRYPVPSY 76 Query: 63 EPLKQAIARRHAADGVSV---REVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPT 119 LK AI + G+ V +V +GNGSDE++ A Q G+ IL P+ + Y Sbjct: 77 TALKAAICK-----GLGVPAGYDVLLGNGSDELITLLSVACAQPGRTILAPEPGFVMYGV 131 Query: 120 YAGLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGII--FPNPNAPTGCLLGLADVERILQ 177 A + +EY VPL D +L L +A +I PN PTG L +ADV RI++ Sbjct: 132 SAKMAGLEYVGVPLCADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTLYEMADVLRIVE 191 Query: 178 -GNPERVVVVDEAYIDFGGHSAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLID 236 P +VVVDEAY F + +P + ++ NL++++T+SK LAG+R+G+ L+ Sbjct: 192 AAAPYGLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSK-LGLAGIRLGYLSAAPALLR 250 Query: 237 ALERVKNSFNSYPLDRPAIAGATAAIEDEDYFRKTCDMVIASRTALAAQLAGL-GFQVLP 295 L++V+ +N L A A +E D + + +R+ALAA+LA L G QV P Sbjct: 251 ELDKVRPPYNVNVLTE---AAALFMLEHLDVLQAQAARLRQARSALAAELAALDGVQVFP 307 Query: 296 SAANFLFVRHPQHDAAQLAAALRADGIIVRHFKSERI--SQFLRITIGTDEDCGVLVEAL 353 SAANFL +R P DA ++ A+L A ++V++ + LRIT+ +D++ + + AL Sbjct: 308 SAANFLLIRVP--DADKVCASLLAHRVLVKNAGRMHVLLQNCLRITVSSDKENAMFLAAL 365 Query: 354 RRHL 357 + L Sbjct: 366 KTAL 369 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 83/264 (31%), Positives = 119/264 (45%), Gaps = 27/264 (10%) Query: 65 LKQAIAR----RHAADGVSVREVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTY 120 L++AIA R+ D E V GS E LAH A L G +L P+ +Y + Sbjct: 110 LRRAIAHWYRSRYEVDFDPDSEAIVTIGSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYG 169 Query: 121 AGLYQVEYRAVPLAE--DF-----TLRSEDYLGHGQAIGGIIFPNPNAPTGCLLGLADVE 173 A + R+V ++ DF E Y I G P+ PT + L E Sbjct: 170 AVIAGANIRSVRMSPGVDFFDELERAVRESYPKPKMMILGF----PSNPTAQCVELEFFE 225 Query: 174 RILQGNPER--VVVVDEAYID--FGGHSAIPLVQRYPNLLVVQ----TLSKSRALAGMRV 225 R+++ E +VV D AY D F G A P + + P V TLSKS +AG R+ Sbjct: 226 RVVKLAREHQILVVHDLAYADITFDGWKA-PSIMQVPGAREVAVEFFTLSKSYNMAGWRI 284 Query: 226 GFAVGHADLIDALERVKNSFNSYPLDRPAIAGATAAIE-DEDYFRKTCDMVIASRTALAA 284 GF VG+A L+ AL R+K S++ Y P A AA+E D+ + R L Sbjct: 285 GFMVGNARLVAALARIK-SYHDYGSFTPVQVAAIAALEGDQSCVEEIRANYERRRNVLVK 343 Query: 285 QLAGLGFQV-LPSAANFLFVRHPQ 307 L G+ V +P A+ +++ R P+ Sbjct: 344 GLHEAGWMVDVPKASMYIWARIPE 367 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,128,105 Number of extensions: 48841 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 161 Number of HSP's successfully gapped: 3 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)