BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0418.p (199 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313980|ref|YP_003778072.1| two component response regulato... 410 e-116 gi|300310565|ref|YP_003774657.1| response regulator containing a... 112 2e-26 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 100 9e-23 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 99 2e-22 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 96 3e-21 >gi|300313980|ref|YP_003778072.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 199 Score = 410 bits (1053), Expect = e-116, Method: Compositional matrix adjust. Identities = 199/199 (100%), Positives = 199/199 (100%) Query: 1 MPVSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMAS 60 MPVSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMAS Sbjct: 1 MPVSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMAS 60 Query: 61 QVHDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYL 120 QVHDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYL Sbjct: 61 QVHDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPFNLAEIVHCHHEYL 120 Query: 121 DGSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAV 180 DGSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAV Sbjct: 121 DGSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAV 180 Query: 181 VVDACLDVVKRSNWRPISN 199 VVDACLDVVKRSNWRPISN Sbjct: 181 VVDACLDVVKRSNWRPISN 199 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 112 bits (279), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 62/184 (33%), Positives = 99/184 (53%), Gaps = 2/184 (1%) Query: 3 VSESNKIYQEVIDAMIAVHRLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQV 62 V S+ + + +D + D T H + A A +I + L +++ + L A+ + Sbjct: 146 VRHSDTLALDSVDLLARAAGYRDPETGEHLTRMANYARIIAARMGLDSQQQELLLNAAPM 205 Query: 63 HDIGKARIPLALLTKPGRISVEEYDVLKTHVQFGYEILNKISFPF--NLAEIVHCHHEYL 120 HDIGK IP +L KPGR+ EE V++ H + G +IL P ++I HHE Sbjct: 206 HDIGKMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPSPLLQTASQIALTHHEKW 265 Query: 121 DGSGYPRGISGDEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSGVRLDAV 180 DGSGYP G+ G++IPL RI+ VAD+ +++++ RPY+ + A ++ QSG D Sbjct: 266 DGSGYPNGLKGEQIPLYGRIVAVADVFDALTSSRPYKPGWSLERASQYLREQSGAHFDPR 325 Query: 181 VVDA 184 V+A Sbjct: 326 CVEA 329 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 100 bits (248), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 51/154 (33%), Positives = 86/154 (55%), Gaps = 1/154 (0%) Query: 22 RLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQVHDIGKARIPLALLTKPGRI 81 +L D YT H L V +GR L L + +HD+GKA IP LL KPG++ Sbjct: 153 KLADEYTYLHSVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKL 212 Query: 82 SVEEYDVLKTHVQFGYEILNK-ISFPFNLAEIVHCHHEYLDGSGYPRGISGDEIPLEARI 140 S EE+ +K H + GY+ L++ + P ++ HHE +DG+GYP G+ I L +R+ Sbjct: 213 SDEEFSQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRM 272 Query: 141 LTVADIVESMSADRPYRKSLGMSAALDEIQRQSG 174 + D+ +++++DRPY+++ + A+ + +G Sbjct: 273 AAICDVYDALTSDRPYKRAWDPAEAISAMASWTG 306 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 99.4 bits (246), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 49/154 (31%), Positives = 85/154 (55%), Gaps = 1/154 (0%) Query: 22 RLTDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQVHDIGKARIPLALLTKPGRI 81 +L D YT H L + +GR L L+ ++ +HD+GKA I +L KPG++ Sbjct: 150 KLNDEYTYLHSVSVCALVIGLGRTLGLSEAACREAGLSGYLHDVGKAFIDDEILNKPGKL 209 Query: 82 SVEEYDVLKTHVQFGYEILNKI-SFPFNLAEIVHCHHEYLDGSGYPRGISGDEIPLEARI 140 + EE+ ++K H + GY+ L + P +++ HHE LDG GYP + + I L AR+ Sbjct: 210 TAEEFALIKRHPELGYQYLIQTPDMPAYASDVCRHHHERLDGRGYPDALPAESITLYARM 269 Query: 141 LTVADIVESMSADRPYRKSLGMSAALDEIQRQSG 174 V D+ ++++++RPY+ + A+ ++ R G Sbjct: 270 TAVCDVYDALTSERPYKHGWDPAEAISQMARWEG 303 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 95.5 bits (236), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 52/163 (31%), Positives = 89/163 (54%), Gaps = 3/163 (1%) Query: 15 DAMIAVHRL--TDGYTPNHEDQTAELAVMIGRHLDLATERIDALHMASQVHDIGKARIPL 72 +A++++ RL D YT H L V + R L + +A +HD+GKA IPL Sbjct: 138 NALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPL 197 Query: 73 ALLTKPGRISVEEYDVLKTHVQFGYEILNKIS-FPFNLAEIVHCHHEYLDGSGYPRGISG 131 +L KP R+S +EY +++ H GY+ L S P + ++ HHE +DGSGYP G Sbjct: 198 EILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQG 257 Query: 132 DEIPLEARILTVADIVESMSADRPYRKSLGMSAALDEIQRQSG 174 ++I + +R+ V D+ ++++++RPY+ + +L + G Sbjct: 258 NQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQG 300 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 560,891 Number of extensions: 21221 Number of successful extensions: 83 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 75 Number of HSP's successfully gapped: 11 Length of query: 199 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 118 Effective length of database: 1,236,335 Effective search space: 145887530 Effective search space used: 145887530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)