BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0417.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313979|ref|YP_003778071.1| major facilitator superfamily p... 261 9e-71 >gi|300313979|ref|YP_003778071.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 400 Score = 261 bits (666), Expect = 9e-71, Method: Compositional matrix adjust. Identities = 129/131 (98%), Positives = 129/131 (98%) Query: 1 MTTKHCIPALPQNHQAWRKIAIPFLFLLLGVVFSTWAARIPAIRDQLQLDAATLALVLLC 60 MTTKHCIPALPQNHQAWRKIAIPFLFLLLGVVFSTWAARIPAIRDQLQLDAATLALVLLC Sbjct: 16 MTTKHCIPALPQNHQAWRKIAIPFLFLLLGVVFSTWAARIPAIRDQLQLDAATLALVLLC 75 Query: 61 GGIGAVGSFPLAAWITGHYGARHSSLYAGLALLFSLPLLALAPGLWTLMAVCTLYGAASS 120 GGIGAVGSFPLAAWITGHYGARHSSLYAGL LL SLPLLALAPGLWTLMAVCTLYGAASS Sbjct: 76 GGIGAVGSFPLAAWITGHYGARHSSLYAGLVLLLSLPLLALAPGLWTLMAVCTLYGAASS 135 Query: 121 CFDVAINALGA 131 CFDVAINALGA Sbjct: 136 CFDVAINALGA 146 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.137 0.550 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 663,182 Number of extensions: 18151 Number of successful extensions: 175 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 2 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 131 (55.1 bits)