BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0409.p (671 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 1340 0.0 gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 397 e-111 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 364 e-101 gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 362 e-101 gi|300310711|ref|YP_003774803.1| carbamoyl-phosphate synthase la... 58 2e-09 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 1340 bits (3469), Expect = 0.0, Method: Compositional matrix adjust. Identities = 661/671 (98%), Positives = 665/671 (99%) Query: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY Sbjct: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 Query: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAEAGLVFIGPPASAIEAMGSKAAAK 120 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACA+AGLVFIGPPASAIEAMGSKAAAK Sbjct: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAK 120 Query: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA 180 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA Sbjct: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA 180 Query: 181 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE 240 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE Sbjct: 181 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE 240 Query: 241 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVT 300 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVT Sbjct: 241 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVT 300 Query: 301 EMITGLDLVEWQLRVAAGQALPLKQAQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMRS 360 EMITGLDLVEWQLRVAAGQALPLKQ QLRIHGHAIEARIYAENPEKGFLPAIGTLTRMRS Sbjct: 301 EMITGLDLVEWQLRVAAGQALPLKQEQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMRS 360 Query: 361 AAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRAL 420 AAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRAL Sbjct: 361 AAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRAL 420 Query: 421 AQYQVVGLATNIAFLQRLVAGQAFSTADLDTGLIERHRDTLFPPPSSPNTETLALAVAAI 480 AQYQVVGLATNIAFLQRLVAGQAFSTA LDTGLIERHRDTLFPPPSSP+TETLALA+AAI Sbjct: 421 AQYQVVGLATNIAFLQRLVAGQAFSTAALDTGLIERHRDTLFPPPSSPDTETLALAIAAI 480 Query: 481 LQHEHAQAERSDPWSQRNGWRLNTRLLRTLRFEHDAGDTTVQLDYAGDGQHWSLQTNGQT 540 LQ EHAQAERSDPWSQRNGWRLNTRLLRTLRFEHDAGDTTVQLDYAGDGQHWSL TNGQT Sbjct: 481 LQQEHAQAERSDPWSQRNGWRLNTRLLRTLRFEHDAGDTTVQLDYAGDGQHWSLLTNGQT 540 Query: 541 HALQLHAADGDTLMISLDGRTLRADVLREGEWLHVFDAHGQRSLRHVDPLAHAGHSEAEG 600 HALQLHAADGDTL+ISLDGRTLRADVLREGEWLHVFDAHGQ SLRHVDPLAHAGHSEAEG Sbjct: 541 HALQLHAADGDTLIISLDGRTLRADVLREGEWLHVFDAHGQHSLRHVDPLAHAGHSEAEG 600 Query: 601 GRLTAPMPGKIVALLVQKGASVAQGTPLLIMEAMKMEHTIAAPADGVVEDLLYAVGDQVA 660 GRLTAPMPGKIVALLVQKGASVA GTPLLIMEAMKMEHTIAAPADGVVEDLLYAVGDQVA Sbjct: 601 GRLTAPMPGKIVALLVQKGASVAHGTPLLIMEAMKMEHTIAAPADGVVEDLLYAVGDQVA 660 Query: 661 EGAQLLEFKAA 671 EGAQLLEFKAA Sbjct: 661 EGAQLLEFKAA 671 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 397 bits (1019), Expect = e-111, Method: Compositional matrix adjust. Identities = 228/472 (48%), Positives = 298/472 (63%), Gaps = 19/472 (4%) Query: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 MF KILIANRGEIA R+ R LGI TV V+S+AD AK+V DESV +GPAP+ SY Sbjct: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSY 60 Query: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAEAGLVFIGPPASAIEAMGSKAAAK 120 L II+AA T AQAIHPGYGFLSENA FA+ ++G VFIGP A I MG K +AK Sbjct: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAK 120 Query: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQ-ADRIGYPVLLKASAGGGGKGMRVVERSEDFDA 179 M +A VP VPG G ++ Q A +IGYPV++KA+ GGGG+GMRVV Sbjct: 121 QAMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 Query: 180 ALASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVL 239 A+ K EA A+FG+ V +EKYL PRH+EIQ+ AD H ++L ERDCS+QRRHQKV+ Sbjct: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAIWLGERDCSMQRRHQKVI 240 Query: 240 EEAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPV 299 EEAPAPG+ + +GE A R + Y GAGT EF+ + FYF+EMNTR+QVEHPV Sbjct: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENE-EFYFIEMNTRVQVEHPV 299 Query: 300 TEMITGLDLVEWQLRVAAGQALPLKQAQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMR 359 TEMITG+D+V+ Q+R+AAG+ L +Q + + GHAIE RI AE+P K F+P+ G +T Sbjct: 300 TEMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRIT--- 355 Query: 360 SAAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRA 419 PG P +R+D+ G + P YD M+ K+I +G R+ A+R MQ A Sbjct: 356 ---------AWHVPGGPG-IRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIA 405 Query: 420 LAQYQVVGLATNIAFLQRLVAGQAFSTADLDTGLIERHRDTLFPPPSSPNTE 471 L++ V G++TNI + L+ F + +E H+ L P+SP + Sbjct: 406 LSEMVVEGISTNIPLHRELMVDARFFEGGTNIHYLE-HK--LSERPASPEAD 454 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 364 bits (934), Expect = e-101, Method: Compositional matrix adjust. Identities = 216/459 (47%), Positives = 287/459 (62%), Gaps = 15/459 (3%) Query: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 MF +LIANRG IACR+ T R L + V VYS+AD + HV D ++ LG PA ++Y Sbjct: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 Query: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAEAGLVFIGPPASAIEAMGSKAAAK 120 L +KI+AAA +GAQAIHPGYGFLSENA FA+AC AG+ F+GP + + G K A+ Sbjct: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 Query: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA 180 AL + VP++ G E L + A R+GYPV+LK++AGGGG GMRV + A Sbjct: 121 ALAREQGVPMLEGTDLLESIDDAL-AAASRVGYPVMLKSTAGGGGIGMRVCRSDAELADA 179 Query: 181 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE 240 S +R +F D V +EKY+ R RH+E+QVF DG G + L RDCSVQRR+QKVLE Sbjct: 180 FDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLE 239 Query: 241 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQ-DGSFYFMEMNTRLQVEHPV 299 E PAP + + A+ AA+ A+AV Y AGTVEF+ + G FYF+E+NTRLQVEH V Sbjct: 240 ETPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGV 299 Query: 300 TEMITGLDLVEWQLRVAAGQALPLKQ--AQLRIHGHAIEARIYAENPEKGFLPAIGTLTR 357 TE + G+DLV W + +AAG PL Q A L+ GHAI+AR+YAE+P + F P G LT Sbjct: 300 TEQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLT- 358 Query: 358 MRSAAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQ 417 AV+F P D +RID V G I+P++DPMIAKLI W P R+AA + Sbjct: 359 -----AVDF-----PPVDGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALD 408 Query: 418 RALAQYQVVGLATNIAFLQRLVAGQAFSTADLDTGLIER 456 AL + G+ TN +L++++A F++ T +E+ Sbjct: 409 AALRATLLYGVETNQHYLRQILADVPFASGQPWTRCLEQ 447 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 362 bits (929), Expect = e-101, Method: Compositional matrix adjust. Identities = 207/453 (45%), Positives = 294/453 (64%), Gaps = 15/453 (3%) Query: 4 KILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESYLCA 63 K+LIANRGEIA R+ A+ LGI TVA SDAD + DE R+GPA A SYL Sbjct: 7 KLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYLNV 66 Query: 64 EKIIAAALATGAQAIHPGYGFLSENAGFAQACAEAGLVFIGPPASAIEAMGSKAAAKALM 123 + ++ AA +GA A+HPGYGFLSENA FA+A AGL+F+GP A I MG KA A+ Sbjct: 67 DALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARRTA 126 Query: 124 EQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAALAS 183 A VP+VPG GE ++ + AD IGYP+L+KASAGGGG+G+R+ + + Sbjct: 127 AAAGVPVVPGSAGELEDLATALACADEIGYPLLIKASAGGGGRGIRMARDATELAREFPL 186 Query: 184 CKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLEEAP 243 + EA A+FG V +E+++ R RHIE+Q+ DG V+LFER+CS+QRR QKVLEEAP Sbjct: 187 AQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEEAP 245 Query: 244 APGMSEERRAAMGEAAVAAARAVGYVGAGTVEFI-ANQDGSFYFMEMNTRLQVEHPVTEM 302 +P ++ +R A+ ++AV A + Y GAGT+E++ ++ G F+F+EMNTR+QVEHPVTEM Sbjct: 246 SPALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVTEM 305 Query: 303 ITGLDLVEWQLRVAAGQALPLKQAQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMRSAA 362 ITG+DLV+ LR+A G+ L L+Q+ +R+ G A+E RI AE+PE+ F P GT+ A Sbjct: 306 ITGIDLVQAMLRIAGGEPLGLQQSDIRMQGAALEMRINAEDPERNFFPCPGTV------A 359 Query: 363 AVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRALAQ 422 +++ G+ +R+++ + G I P+YD ++AKL+V G DR A+ Q A+ Sbjct: 360 ELQWPQGEG-------IRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLA 412 Query: 423 YQVVGLATNIAFLQRLVAGQAFSTADLDTGLIE 455 ++ G+AT ++ Q L+A +A DTG +E Sbjct: 413 TRITGMATTLSLHQWLLADARVQSARFDTGALE 445 >gi|300310711|ref|YP_003774803.1| carbamoyl-phosphate synthase large subunit [Herbaspirillum seropedicae SmR1] Length = 1077 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 50/195 (25%), Positives = 88/195 (45%), Gaps = 22/195 (11%) Query: 141 SGLLHSQADR------IGYPVLLKASAGGGGKGMRVVERSEDFDAALASCKREAIASFGD 194 SG+ H+ + IG+PV+++ S GG G + +E+F+ CKR AS Sbjct: 146 SGVSHTMEESWAVQKTIGFPVIIRPSFTMGGTGGGIAYNAEEFETI---CKRGLEAS-PT 201 Query: 195 DRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQ------KVLEEAPAPGMS 248 +L+E+ L + E++V D +C+ + CS++ + APA ++ Sbjct: 202 SELLIEESLIGWKEYEMEVVRDKADNCIIV----CSIENLDPMGVHTGDSITVAPAQTLT 257 Query: 249 EERRAAMGEAAVAAARAVGY-VGAGTVEFIANQ-DGSFYFMEMNTRLQVEHPVTEMITGL 306 ++ M A++A R +G G V+F N DG +EMN R+ + TG Sbjct: 258 DKEYQIMRNASLAVLREIGVDTGGSNVQFSVNPADGRMIVIEMNPRVSRSSALASKATGF 317 Query: 307 DLVEWQLRVAAGQAL 321 + + ++A G L Sbjct: 318 PIAKIAAKLAVGFTL 332 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,047,563 Number of extensions: 84560 Number of successful extensions: 343 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 334 Number of HSP's successfully gapped: 6 Length of query: 671 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 579 Effective length of database: 1,184,250 Effective search space: 685680750 Effective search space used: 685680750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)